The expression of SARS-CoV M gene in P. Pastoris and the diagnostic utility of the expression product

Journal of Virological Methods

Volumn 122, Issue 1, 2004, Pages 105-111

  Han, Xueqing ^a,b   Bartlam, Mark ^a   Jin, Ying Hua ^a   Liu, Xiangtao ^b   He, Xiaojing ^a   Cai, Xuepeng ^b   Xie, Qingqe ^b   Rao, Zihe ^a  

^a INSTITUTE OF BIOPHYSICS   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Antigenicity; Recombinant M protein; SARS coronavirus

Indexed keywords

MEMBRANE PROTEIN; RECOMBINANT M PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CASE REPORT; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; IMMUNOFLUORESCENCE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SARS CORONAVIRUS; VIRUS GENE; WESTERN BLOTTING; YEAST;

ADULT; AMINO ACID SEQUENCE; ANTIBODIES, VIRAL; BLOTTING, WESTERN; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GENES, VIRAL; GENETIC VECTORS; HUMANS; IMMUNOLOGIC TECHNIQUES; MALE; MOLECULAR SEQUENCE DATA; PICHIA; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEVERE ACUTE RESPIRATORY SYNDROME; VIRAL MATRIX PROTEINS;

CORONAVIRUS; PICHIA; PICHIA PASTORIS; SARS CORONAVIRUS; VECTORS;

EID: 5644254409     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2004.08.015     Document Type: Article

References (24)

1. E. Corse, and C.E. Machamaer Infectious bronchitis virus E protein is targeted to the Golgi complex and directs release of virus-like particles J. Virol. 74 2000 4319 4326
2. R.H. David, and M.C. James Pichia Protocols 1998 Humana Press Inc. New Jersey
3. C.A. de Haan, L. Kuo, P.S. Masters, H. Vennema, and P.J. Rottier Coronavirus particle assembly: primary structure requirements of the membrane protein J. Virol. 72 1998 6838 6850
4. C.A. de Haan, H. Vennema, and P.J. Rottier Assembly of the coronavirus envelope: homotypic interactions between the M proteins J. Virol. 74 2000 4967 4978
5. C.A. de Haan, M. de Wit, L. Kuo, C. Montalto, P.S. Masters, S.R. Weiss, and P.J. Rottier O-glycosylation of the mouse hepatitis coronavirus membrane protein Virus Res. 82 2002 77 81
6. G. Elia, G. Fiermonte, A. Pratelli, V. Martella, M. Camero, F. Cirone, and C. Buonavoglia Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus J. Virol. Methods 109 2003 139 142
7. L.S. Grinner, and J.F. Tschopp Size distribution and general structural features of N-linked oligosaccharides from the methyltrophic yeast Picha pastoris Yeast 5 1989 107 115
8. M. Guan, H.Y. Chen, S.Y. Foo, Y.J. Tan, P.Y. Goh, and S.H. Wee Recombinant protein-based enzyme-linked imunosorbent assay and immunochromatographic tests for detection of immunoglobulin G antibodies to severe acute respiratory syndrome (SARS) coronavirus in SARS patients Clin. Diag. Lab. Immunol. 11 2004 287 291
9. Q. He, K.H. Chong, H.H. Chng, B. Leung, A.E. Ling, T. Wei, S.W. Chan, E. Ooi, and J. Kwang Development of a Western blot assay for detection of antibodies against coronavirus causing severe acute respiratory syndrome Clin. Diag. Lab. Immunol. 11 2004 417 422
10. T.G. Ksiazek, D. Erdman, C.S. Goldsmith, S.R. Zaki, T. Peret, S. Emery, S. Tong, C. Urbani, J.A. Comer, W. Lim, P.E. Rollin, S.F. Dowell, A.E. Ling, C.D. Humphrey, W.J. Shieh, J. Guarner, C.D. Paddock, P. Rota, B. Fields, J. DeRisi, J.Y. Yang, N. Cox, J.M. Hughes, J.W. LeDuc, W.J. Bellini, L.J. Anderson, and S.W. Group A novel coronavirus associated with severe acute respiratory syndrome N. Engl. J. Med. 348 2003 1953 1966
11. M.A. Kukuruzinska, M.L. Bergh, and B.J. Jackson Protein glycosylation in yeast Annu. Rev. Biochem. 56 1987 915 944
12. R. Molekamp, and W.J. Spaan Identification of a specific interaction between the mouse hepatitis virus A59 nucleocapsid protein and packaging signal Virology 239 1997 78 86
13. K. Narayanan, and S. Makino Cooperation of an RNA packaging signal and a viral envelope protein in coronavirus RNA packaging J. Virol. 75 2001 9059 9067
14. L.L. Poon, K.H. Chan, O.K. Wong, W.C. Yam, K.Y. Yuen, Y. Guan, Y.M. Lo, and J.S. Peiris Early diagnosis of SARS coronavirus infection by real time RT-PCR J. Clin. Virol. 28 2003 233 238
15. L.L. Poon, K.H. Chan, O.K. Wong, T.K. Cheung, I. Ng, B. Zheng, W.H. Seto, K.U. Yuen, Y. Guan, and J.S. Peiris Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays Clin. Chem. 50 2004 67 72
16. S.M. Poutanen, D.E. Low, B. Henry, S. Finkelstein, D. Rose, K. Green, R. Tellier, R. Draker, D. Adachi, M. Ayers, A.K. Chan, D.M. Skowronski, I. Salit, A.E. Simor, A.S. Slutsky, P.W. Doyle, M. Krajden, M. Petric, R.C. Brunham, and A.J. McGeer Identification of severe acute respiratory syndrome in Canada N. Engl. J. Med. 348 2003 1995 2005
17. S. Riley, C. Fraser, C.A. Donnelly, A.C. Ghani, L.J. Abu-Raddad, A.J. Hedley, G.M. Leung, L.M. Ho, T.H. Lam, T.Q. Thach, P. Chau, K.P. Chan, S.V. Lo, P.Y. Leung, T. Tsang, W. Ho, K.H. Lee, E.M. Lau, N.M. Ferguson, and R.M. Anderson Transmission dynamics of the etiological agent of SARS in Hong Kong: impact of public health interventions Science 300 2003 1961 1966
18. M.A. Romanos, C.A. Scorer, and J.J. Clare Foreign gene expression in yeast: a review Yeast 8 1992 423 488
19. Rota, P.A., Oberste, M.S., Monroe, S.S., Nix, W.A., Campagnoli, R., Icenogle, J.P., Peneranda, S., Bankamp, B., Maher, K., Chen, M.H., Tong, S., Tamin, A., Lowe, L., Frace, M., DeRisi, J.L., Chen, Q., Wang, D., Erdman, D.D., Peret, T.C., Burns, C., Kziazek, T.G., Rollin, P.E., Sanchez, A., Liffick, S., Holloway, B., Limor, J., McCaustland, K., Olsen-Rasmussen, M., Fouchier, R., Gunther, S., Osterhaus, A.D., Drosten, C., Pallansch, M.A., Anderson, L.J. and Bellini, W.J., 2003. Characterization of a novel coronavirus associated with severe acute respiratory science. Science 300, 1394-1399.
20. P.J.M. Rottier The coronavirus membrane protein. S.G. Siddell The Coronaviridae 1995 Plenum Press New York 115 139
21. Y. Shi, Y. Yi, P. Li, T. Kuang, L. Li, M. Dong, Q. Ma, and C. Cao Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay J. Clin. Microbiol. 41 2003 5781 5782
22. H. Vennema, G.J. Godecke, J.W. Rossen, W.F. Voorhout, M.C. Horzinek, D.J. Opstelten, and P.J. Rottier Nucleocapsid-independent assembly of coronavirus-like particles by co-expression of viral envelope protein genes EMBO J. 15 1996 2020 2028
23. R.D. Woods, R. Wesley, and P.A. Kapke Complement-dependent neutralization of transmissible gastroenteritis virus by monoclonal antibodies Adv. Exp. Med. Biol. 218 1987 493 500
24. Y. Yi, C. Li, Y. Shi, L. Li, P. Li, W. Huang, S. Wang, Q. Ma, and C. Cao Over-expression in Escherichia coli and purification of nucleocapsid and membrane protein of SARS coronavirus Chin. J. Biotechnol. 19 2003 392 396