Study of interaction between Smad7 and DNA by single-molecule force spectroscopy

Biochemical and Biophysical Research Communications

Volumn 377, Issue 4, 2008, Pages 1284-1287

  Shi, Xiaoli ^a   Chen, Feng ^b   Yu, Junping ^a   Xu, Yongchun ^a   Zhang, Suping ^b   Chen, Ye Guang ^b   Fang, Xiaohong ^a  

^a PEKING UNIVERSITY   (China)

^b TSINGHUA UNIVERSITY   (China)

Author keywords

Atomic force microscopy; Protein DNA interaction; Single molecule force spectroscopy; Smad4; Smad7; TGF

Indexed keywords

ACTIVIN; CAGA PROTEIN; DNA; OLIGONUCLEOTIDE; SMAD4 PROTEIN; SMAD7 PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DNA BINDING; DNA RESPONSIVE ELEMENT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN DNA INTERACTION; PROTEIN DOMAIN; SPECTROSCOPY;

BASE SEQUENCE; DNA; MICROSCOPY, ATOMIC FORCE; PLASMINOGEN ACTIVATOR INHIBITOR 1; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RESPONSE ELEMENTS; SMAD7 PROTEIN;

EID: 56349113273     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.10.145     Document Type: Article

References (16)

1. Massague J., and Chen Y.G. Controlling TGF-signaling. Genes Dev. 14 (2000) 627-644
2. Massagué J., Seoane J., and Wotton D. Smad transcription factors. Genes Dev. 19 (2005) 2783-2810
3. Dennler S., Itoh S., Vivien D., ten Dijke P., Huet S., and Gauthier J.M. Direct binding of Smad3 and Smad4 to critical TGF-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 gene. EMBO J. 17 (1998) 3091-3100
4. Derynck R., and Zhang Y.E. Smad-dependent and Smad-independent pathways in TGF-bold beta family signaling. Nature 425 (2003) 577-584
5. Zhang S., Fei T., Zhang L., Zhang R., Chen F., Ning Y., Han Y., Feng X.H., Meng A., and Chen Y.G. Smad7 antagonizes transforming growth factor beta signaling in the nucleus by interfering with functional Smad-DNA complex formation. Mol. Cell. Biol. 27 (2007) 4488-4499
6. Heldin C.H., Miyazono K., and ten Dijke P. TGF-[β] signalling from cell membrane to nucleus through SMAD proteins. Nature 390 (1997) 465-471
7. Shi Y. Structural insights on Smad function in TGF-b signaling. Bioessays 23 (2001) 223-232
8. Feng X.H., and Derynck R. Specificity and versatility in tgf-β signaling through Smads. Annu. Rev. 21 (2005) 659-693
9. Muller D.J., and Dufrene Y.F. Atomic force microscopy as a multifunctional molecular toolbox in nanobiotechnology. Nat. Nano. 3 (2008) 261-269
10. Florin E.L., Moy V.T., and Gaub H.E. Adhesion forces between individual ligand-receptor pairs. Science 264 (1994) 415
11. Willemsen O.H., Snel M.M.E., Cambi A., Greve J., De Grooth B.G., and Figdor C.G. Biomolecular interactions measured by atomic force microscopy. Biophys. J. 79 (2000) 3267-3281
12. Jiang Y., Zhu C., Ling L., Wan L., Fang X., and Bai C. Specific aptamer-protein interaction studied by atomic force microscopy. Anal. Chem. 75 (2003) 2112-2116
13. Yu J., Wang Q., Shi X., Ma X., Yang H., Chen Y.G., and Fang X. Single-molecule force spectroscopy study of interaction between transforming growth factor β and its receptor in living cells. J. Phys. Chem. B 111 (2007) 13619-13625
14. Sun S., Yu J., Chen F., Zhao T., Fang X., Li Y., and Sui S. TINY a dehydration-responsive element (DRE)-binding protein-like transcription factor connecting the DRE-and ethylene-responsive element-mediated signaling patheways in Arabidopsis. J. Biol. Chem. 283 (2008) 6261-6271
15. Huang H.C., Murtaugh L.C., Vize P.D., and Whitman M. Identification of a potential regulator of early transcriptional responses to mesoderm inducers in the frog embryo. EMBO J. 14 (1995) 5965-5973
16. Hata A., Lo R.S., Wotton D., Lagna G., and Massague J. Mutations increasing autoinhibition inactivate tumour suppressors Smad2 and Smad4. Nature 388 (1997) 82-87