Construction of a new polycistronic vector for over-expression and rapid purification of human hemoglobin

Plasmid

Volumn 61, Issue 1, 2009, Pages 71-77

  Domingues, Elisa ^a   Brillet, Thomas ^a   Vasseur, Corinne ^a   Agier, Virginie ^a   Marden, Michael C ^a   Baudin Creuza, Véronique ^a  

^a INSERM   (France)

Author keywords

Co expression pGEX vector; GST PreScission protease; Polycistronic system; Protein engineering; Rapid affinity purification; Recombinant Hb A

Indexed keywords

CARBON MONOXIDE; GLUTATHIONE; GLUTATHIONE TRANSFERASE; HEMOGLOBIN A; METHIONINE; PLASMID VECTOR;

AFFINITY CHROMATOGRAPHY; ALLOSTERISM; ARTICLE; BINDING AFFINITY; BINDING KINETICS; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PURIFICATION; SOLUBILITY; STRUCTURE ACTIVITY RELATION;

ALPHA-GLOBINS; BETA-GLOBINS; CARBON MONOXIDE; CLONING, MOLECULAR; GENE EXPRESSION; GENETIC VECTORS; GLUTATHIONE TRANSFERASE; HEMOGLOBIN A; HUMANS; KINETICS; RECOMBINANT FUSION PROTEINS;

EID: 56349083255     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2008.09.006     Document Type: Article

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