Proteomic analysis of CTGF-activated lung fibroblasts: Identification of IQGAP1 as a key player in lung fibroblast migration

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 295, Issue 4, 2008, Pages

  Bogatkevich, Galina S ^a   Ludwicka Bradley, Anna ^a   Singleton, C Beth ^a   Bethard, Jennifer R ^a   Silver, Richard M ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

Cell migration; Connective tissue growth factor; Lung fibrosis; Scleroderma

Indexed keywords

1,4 DIAMINO 1,4 BIS(2 AMINOPHENYLTHIO) 2,3 DICYANOBUTADIENE; CALDESMON; COLLAGEN; CONNECTIVE TISSUE GROWTH FACTOR; CYTOSKELETON PROTEIN; EZRIN; GLUCOSE REGULATED PROTEIN 78; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN U; IQ MOTIF CONTAINING GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN 1; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MOESIN; PHOSPHOPROTEIN; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; PROTEINASE; PROTEOME; SCAFFOLD PROTEIN; SMALL INTERFERING RNA; VALOSIN CONTAINING PROTEIN; VINCULIN; IMMEDIATE EARLY PROTEIN; SIGNAL PEPTIDE; VIMENTIN;

ARTICLE; CELL ACTIVATION; CELL ISOLATION; CELL MIGRATION; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENDOTHELIUM CELL; ENZYME ASSAY; EPITHELIUM CELL; GEL ELECTROPHORESIS; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERSTITIAL LUNG DISEASE; LIQUID CHROMATOGRAPHY; LUNG FIBROBLAST; LUNG FIBROSIS; LUNG PARENCHYMA; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEOMICS; SCLERODERMA; TISSUE INJURY; TISSUE REPAIR; WOUND HEALING; AUTOPSY; CELL MOTION; CULTURE TECHNIQUE; DOWN REGULATION; DRUG EFFECT; FIBROBLAST; GENETIC TRANSFECTION; GENETICS; LUNG; PHYSIOLOGY; SYSTEMIC SCLEROSIS; UPREGULATION;

AUTOPSY; CELL CULTURE TECHNIQUES; CELL MOVEMENT; DOWN-REGULATION; FIBROBLASTS; HUMANS; IMMEDIATE-EARLY PROTEINS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; LUNG; PROTEOMICS; SCLERODERMA, SYSTEMIC; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSFECTION; UP-REGULATION; VIMENTIN; WOUND HEALING;

EID: 56149092270     PISSN: 10400605     EISSN: 15221504     Source Type: Journal    
DOI: 10.1152/ajplung.00530.2007     Document Type: Article

References (38)

1. Bhogal RK, Stoica CM, McGaha TL, Bona CA. Molecular aspects of regulation of collagen gene expression in fibrosis. J Clin Immunol 25: 592-603, 2005.
2. Bogatkevich GS, Ludwicka-Bradley A, Nietert PJ, Silver RM. Scleroderma lung fibroblasts: contractility and connective tissue growth factor. In: Tissue Repair, Contraction and the Myofibroblast, edited by Gabbiani G, Chaponnier C, Desmouliere A. Georgetown, TX: Landes Bioscience, 2006, p. 25-31.
3. Bogatkevich GS, Gustilo E, Oates JC, Feghali-Bostwick C, Harley RA, Silver RM, Ludwicka-Bradley A. Distinct PKC isoforms mediate cell survival and DNA synthesis in thrombin-induced myofibroblasts. Am J Physiol Lung Cell Mol Physiol 288: L190-L201, 2005.
4. Bonniaud P, Margetts PJ, Kolb M, Haberberger T, Kelly M, Robertson J, Gauldie J. Adenoviral gene transfer of connective tissue growth factor in the lung induces transient fibrosis. Am J Respir Crit Care Med 168: 770-778, 2003.
5. Braiman-Wiksman L, Solomonik I, Spira R, Tennenbaum T. Novel insights into wound healing sequence of events. Toxicol Pathol 35: 767-779, 2007.
6. Brown MD, Sacks DB. IQGAP1 in cellular signaling: bridging the GAP. Trends Cell Biol 16: 242-249, 2006.
7. Buckingham RB, Prince RK, Rodnan GP. Progressive systemic sclerosis dermal fibroblasts synthesise increased amounts of glycosaminoglycans. J Lab Clin Med 101: 659-669, 1983.
8. Candiano G, Bruschi M, Pedemonte N, Musante L, Ravazzolo R, Liberatori S, Bini L, Galietta LJ, Zegarra-Moran O. Proteomic analysis of the airway surface liquid: modulation by proinflammatory cytokines. Am J Physiol Lung Cell Mol Physiol 292: L185-L198, 2007.
9. Chujo S, Shirasaki F, Kawara S, Inagaki Y, Kinbara T, Inaoki M, Takigawa M, Takehara K. Connective tissue growth factor causes persistent proα2(I) collagen gene expression induced by transforming growth factor-α in a mouse fibrosis model. J Cell Physiol 203: 447-456, 2005.
10. Crean JK, Finlay D, Murphy M, Moss C, Godson C, Martin F, Brady HR. The role of p42/44 MAPK and protein kinase B in connective tissue growth factor induced extracellular matrix protein production, cell migration, and actin cytoskeletal rearrangement in human mesangial cells. J Biol Chem 277: 44187-44194, 2002.
11. Falk R, Ramström M, Ståhl S, Hober S. Approaches for systematic proteome exploration. Biomol Eng 24: 155-168, 2007.
12. Fonseca C, Lindahl GE, Ponticos M, Sestini P, Renzoni EA, Holmes AM, Spagnolo P, Pantelidis P, Leoni P, McHugh N, Stock CJ, Shi-Wen X, Denton CP, Black CM, Welsh KI, du Bois RM, Abraham DJ. A polymorphism in the CTGF promoter region associated with systemic sclerosis. N Engl J Med 357: 1210-1220, 2007.
13. Frazier K, Williams S, Kothapalli D, Klapper H, Grotendorst GR. Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor. J Invest Dermatol 107: 404-411, 1996.
14. Fukata M, Watanabe T, Noritake J, Nakagawa M, Yamaga M, Kuroda S, Matsuura Y, Iwamatsu A, Perez F, Kaibuchi K. Rac1 and Cdc42 capture microtubules through IQGAP1 and CLIP-170. Cell 109: 873-885, 2002.
15. Fukuda Y, Basset F, Ferrans VJ, Yamanaka N. Significance of early intra-alveolar fibrotic lesions and integrin expression in lung biopsy specimens from patients with idiopathic pulmonary fibrosis. Hum Pathol 26: 53-61, 1995.
16. Gore-Hyer E, Shegogue D, Markiewicz M, Lo S, Hasen-Martin D, Greene E, Grotendorst G, Trojanowska M. TGFβ and CTGF have overlapping and distinct fibrogenic effects on human renal cells. Am J Physiol Renal Physiol 283: F707-F716, 2002.
17. Grotendorst GR, Rahmanie H, Duncan MR. Combinatorial signaling pathways determine fibroblast proliferation and myofibroblast differentiation. FASEB J 18: 469-479, 2004.
18. Hai CM, Gu Z. Caldesmon phosphorylation in actin cytoskeletal remodeling. Eur J Cell Biol 85: 305-309, 2006.
19. Heng EC, Huang Y, Black SA Jr, Trackman PC. CCN2, connective tissue growth factor, stimulates collagen deposition by gingival fibroblasts via module 3 and alpha6- and beta1 integrins. J Cell Biochem 98: 409-420, 2006.
20. Highland KB, Silver RM. Clinical aspects of lung involvement: lessons from idiopathic pulmonary fibrosis and the scleroderma lung study. Curr Rheumatol Rep 7: 135-141, 2005.
21. Ho YD, Joyal JL, Li Z, Sacks DB. IQGAP1 integrates Ca2-/calmodulin and Cdc42 signaling. J Biol Chem 274: 464-470, 1999.
22. Jimenez SA, Derk CT. Following the molecular pathways toward an understanding of the pathogenesis of systemic sclerosis. Ann Intern Med 140: 37-50, 2004.
23. Leask A, Holmes A, Abraham DJ. Connective tissue growth factor: a new and important player in the pathogenesis of fibrosis. Curr Rheumatol Rep 4: 136-142, 2002.
24. LeRoy EC. Increased collagen synthesis by scleroderma skin fibroblasts in vitro. A possible defect in regulation or activation of scleroderma fibroblasts. J Clin Invest 54: 880-889, 1974.
25. Lopez JL. Two-dimensional electrophoresis in proteome expression analysis. J Chromatogr B Analyt Technol Biomed Life Sci 849: 190-202, 2007.
26. Macias MJ, Wiesner S, Sudol M. WW and SH3 domains, two different scaffolds to recognize proline-rich ligands. FEBS Lett 513: 30-37, 2002.
27. Mataraza JM, Li Z, Jeong HW, Brown MD, Sacks DB. Multiple proteins mediate IQGAP1-stimulated cell migration. Cell Signal 19: 1857-1865, 2007.
28. Mateer SC, Morris LE, Cromer DA, Benseñor LB, Bloom GS. Actin filament binding by a monomeric IQGAP1 fragment with a single calponin homology domain. Cell Motil Cytoskeleton 58: 231-241, 2004.
29. Myllyharju J. Prolyl 4-hydroxylases, the key enzymes of collagen biosynthesis. Matrix Biol 22: 15-24, 2003.
30. Rhoads DS, Guan JL. Analysis of directional cell migration on defined FN gradients: role of intracellular signaling molecules. Exp Cell Res 313: 3859-3867, 2007.
31. Sato S, Nagaoka T, Hasegawa M, Tamatani T, Nakanishi T, Tagikawa M, Takehara K. Serum levels of connective tissue growth factor are elevated in patients with systemic sclerosis: association with extent of skin sclerosis and severity of pulmonary fibrosis. J Rheumatol 27: 149-154, 2000.
32. Shi-Wen X, Chen Y, Denton CP, Eastwood M, Renzoni EA, Bou-Gharios G, Pearson JD, Dashwood M, du Bois RM, Black CM, Leask A, Abraham DJ. Endothelin-1 promotes myofibroblast induction through the ETA receptor via a rac/phosphoinositide 3-kinase/Akt-dependent pathway and is essential for the enhanced contractile phenotype of fibrotic fibroblasts. Mol Biol Cell 15: 2707-2719, 2004.
33. Shi-Wen X, Pennington D, Holmes A, Leask A, Bradham D, Beauchamp JR, Fonseca C, du Bois RM, Martin GR, Black CM, Abraham DJ. Autocrine overexpression of CTGF maintains fibrosis: RDA analysis of fibrosis genes in systemic sclerosis. Exp Cell Res 259: 213-224, 2000.
34. Sinha A, Singh C, Parmar D, Singh MP. Proteomics in clinical interventions: achievements and limitations in biomarker development. Life Sci 80: 1345-1354, 2007.
35. Vedrenne C, Hauri HP. Morphogenesis of the endoplasmic reticulum: Beyond active membrane expansion. Traffic 7: 639-646, 2006.
36. Xiao R, Liu FY, Luo JY, Yang XJ, Wen HQ, Su YW, Yan KL, Li YP, Liang YS. Effect of small interfering RNA on the expression of connective tissue growth factor and type I and III collagen in skin fibroblasts of patients with systemic sclerosis. Br J Dermatol 155: 1145-1153, 2006.
37. Yamaoka-Tojo M, Ushio-Fukai M, Hilenski L, Dikalov SI, Chen YE, Tojo T, Fukai T, Fujimoto M, Patrushev NA, Wang N, Kontos CD, Bloom GS, Alexander RW. IQGAP1, a novel vascular endothelial growth factor receptor binding protein, is involved in reactive oxygen species-dependent endothelial migration and proliferation. Circ Res 95: 276-283, 2004.
38. Zhang C, Meng X, Zhu Z, Liu J, Deng A. Connective tissue growth factor regulates the key events in tubular epithelial to myofibroblast transition in vitro. Cell Biol Int 28: 863-873, 2004.