A circularly permuted β-lactamase as a novel reporter for evaluation of protein cyclization efficiency

Journal of Microbiology

Volumn 46, Issue 4, 2008, Pages 456-461

  Kwon, Jeong Seon ^a   Bal, Jyotiranjan ^a   Hwang, Hai Min ^a   Kim, Jeong Yoon ^a  

^a Chungnam National University   (South Korea)

Author keywords

Circularly permuted lactamase; Protein cyclization; Split intein

Indexed keywords

ESCHERICHIA COLI; SYNECHOCYSTIS; SYNECHOCYSTIS SP.;

BETA LACTAMASE; BETA LACTAMASE TEM 1; BETA-LACTAMASE TEM-1; INTEIN;

ARTICLE; BIOCHEMISTRY; CYCLIZATION; ESCHERICHIA COLI; GENETIC PROCEDURES; GENETICS; METABOLISM; METHODOLOGY; PROTEIN PROCESSING; SYNECHOCYSTIS;

BETA-LACTAMASES; BIOCHEMISTRY; CYCLIZATION; ESCHERICHIA COLI; GENETIC TECHNIQUES; INTEINS; PROTEIN SPLICING; SYNECHOCYSTIS;

EID: 56149088769     PISSN: 12258873     EISSN: 12258873     Source Type: Journal    
DOI: 10.1007/s12275-008-0106-2     Document Type: Article

References (21)

1. J.A. Camarero D. Fushman D. Cowburn T.W. Muir 2001 Peptide chemical ligation inside living cells: In vivo generation of a circular protein domain Bioorg. Med. Chem. 9 2479 2484
2. J.A. Camarero T.W. Muir 1999 Biosynthesis of a head-to-tail cyclized protein with improved biological activity J. Am. Chem. Soc. 121 5597 5598
3. T.C. Evans Jr. D. Martin R. Kolly D. Panne L. Sun I. Ghosh L. Chen J. Benner X.Q. Liu M.Q. Xu 2000 Protein trans-splicing and cyclization by a naturally split intein from the dnaE gene of Synechocystis species PCC6803 J. Biol. Chem. 275 9091 9094
4. A.R. Horswill S.J. Benkovic 2005 Cyclic peptides, a chemical genetics tool for biologists Cell Cycle 4 552 555
5. H. Iwai A. Pluckthun 1999 Circular beta-lactamase: Stability enhancement by cyclizing the backbone FEBS Lett. 459 166 172
6. U.K. Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage t4 Nature 227 680 685
7. O. Massidda G.M. Rossolini G. Satta 1991 The Aeromonas hydrophila cphA gene: Molecular heterogeneity among class B metallo-beta-lactamases J. Bacteriol. 173 4611 4617
8. K.V. Mills B.M. Lew S. Jiang H. Paulus 1998 Protein splicing in trans by purified n-and c-terminal fragments of the mycobacterium tuberculosis reca intein Proc. Natl. Acad. Sci. USA 95 3543 3548
9. F.B. Perler 2002 Inbase: The intein database Nucleic Acids Res. 30 383 384
10. F.B. Perler E.O. Davis G.E. Dean F.S. Gimble W.E. Jack N. Neff C.J. Noren J. Thorner M. Belfort 1994 Protein splicing elements: Inteins and exteins-a definition of terms and recommended nomenclature Nucleic Acids Res. 22 1125 1127
11. F.A. Rubin D.H. Smith 1973 Characterization of R factor betalactamases by the acidimetric method Antimicrob. Agents Chemother. 3 68 73
12. C.P. Scott E. Abel-Santos M. Wall D.C. Wahnon S.J. Benkovic 1999 Production of cyclic peptides and proteins in vivo Proc. Natl. Acad. Sci. USA 96 13638 13643
13. C. Siebold B. Erni 2002 Intein-mediated cyclization of a soluble and a membrane protein in vivo: Function and stability Biophys. Chem. 96 163 171
14. J.M. Spotts R.E. Dolmetsch M.E. Greenberg 2002 Time-lapse imaging of a dynamic phosphorylation-dependent protein-protein interaction in mammalian cells Proc. Natl. Acad. Sci. USA 99 15142 15147
15. P.L. Starokadomskyy 2007 Protein splicing Mol. Biol. 41 278 293
16. M. Vanhove X. Raquet J.M. Frere 1995 Investigation of the folding pathway of the tem-1 beta-lactamase Proteins 22 110 118
17. E. Welker L. Hathaway G. Xu M. Narayan L. Pradeep H.C. Shin H.A. Scheraga 2007 Oxidative folding and n-terminal cyclization of onconase Biochemistry 46 5485 5493
18. N.K. Williams P. Prosselkov E. Liepinsh I. Line A. Sharipo D.R. Littler P.M. Curmi G. Otting N.E. Dixon 2002 In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. Pcc6803 Dnab mini-intein J. Biol. Chem. 277 7790 7798
19. H. Wu M.Q. Xu X.Q. Liu 1998 Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein Biochim. Biophys. Acta. 1387 422 432
20. M.Q. Xu T.C. Evans 2001 Intein-mediated ligation and cyclization of expressed proteins Methods 24 257 277
21. R. Zahn A. Pluckthun 1994 Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. II. GroEL recognizes thermally unfolded mature beta-lactamase J. Mol. Biol. 242 165 174