Substrate specificity of thymidine phosphorylase of E. coli: Role of hydroxyl groups

Nucleosides, Nucleotides and Nucleic Acids

Volumn 27, Issue 12, 2008, Pages 1211-1214

  Panova, Natalya G ^a   Alexeev, Cyril S ^a   Polyakov, Konstantin M ^a   Gavryushov, Sergei A ^a   Kritzyn, Anatoliy M ^a   Mikhailov, Sergey N ^a  

^a ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

Author keywords

Substrate specificity; Thymidine derivatives; Thymidine phosphorlase

Indexed keywords

HYDROXYL GROUP; PYRIMIDINE NUCLEOSIDE; THYMIDINE PHOSPHORYLASE;

ARTICLE; CHEMICAL REACTION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; HYDROGEN BOND;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HYDROGEN BONDING; KINETICS; SUBSTRATE SPECIFICITY; THYMIDINE PHOSPHORYLASE;

EID: 55949122473     PISSN: 15257770     EISSN: 15322335     Source Type: Journal    
DOI: 10.1080/15257770802257895     Document Type: Article

References (9)

1. Pugmire, M.J.; Ealick, S.E. Structural analyses reveal two distinct families of nucleoside phosphorylases. Biochem. J. 2002, 361, 1-25.
2. Edwards, P.N. A kinetic, modeling and mechanistic re-analysis of thymidine phosphorylase and some related enzymes. J. Enz. Inhib. Med. Chem. 2006, 21, 483-518.
3. Walter, M.R.; Cook, W.J.; Cole, L.B.; Short, S.A.; Koszalka, G.W.; Krenitsky, T.A.; Ealick, S.E. Three-dimensional structure of thymidine phosphorylase from Escherichia coli at 2.8 A resolution. J. Biol. Chem. 1990, 265, 14016-14022.
4. Pugmire, M.J.; Cook, W.J.; Jasanoff, A.; Walter, M.R.; Ealick, S.E. Structural and theoretical studies suggest domain movement produces an active conformation of thymidine phosphorylase. J. Mol. Biol. 1998, 281, 285-299.
5. Takebayashi, Y.; Yamada, K.; Miyadera, K.; Sumizawa, T.; Furukawa, T.; Kinoshita, F.; Aoki, D.; Okumura, H.; Yamada, Y.; Akiyama, S.; Aikou, T. The activity and expression of thymidine phosphorylase in human solid tumours. Eur. J. Cancer 1996, 32A, 1227-1232.
6. Utagawa, T. Enzymatic preparation of nucleoside antibiotics. J. Mol. Catalysis B 1999, 6, 215-222.
7. Barai, V.N.; Zinchenko, A.I.; Eroshevskaia, L.A.; Kalinichenko, E.N.; Kulak, T.I.; Mikhailopulo, I.A. A Universal Biocatalyst for the Preparation of Base- and Sugar-Modified Nucleosides via an Enzymatic Transglycosylation. Helvetica Chimica Acta 2002, 85, 1901-1908.
8. Panova, N.G.; Alexeev, C.S.; Kuzmichov, A.S.; Shcheveleva, E.V.; Gavryushov, S.A.; Polyakov, K.M.; Kritzyn, A.M.; Mikhailov, S.N.; Esipov, R.S.; Miroshnikov, A.I. Substrate specificity of E. coli thymidine phosphorylase. Biochemistry (Moscow) 2007, 72, 21-28.
9. Rick, S.W.; Abashkin, Y.G.; Hilderbrandt, R.L.; Burt, S.K. Computational studies of the domain movement and the catalytic mechanism of thymidine phosphorylase. Proteins 1999, 37, 242-252.