A comparative study of cell death using electrical capacitance measurements and dielectrophoresis

Enzyme and Microbial Technology

Volumn 43, Issue 7, 2008, Pages 523-530

  Patel, Pareshkumar M ^a   Bhat, Aditya ^a   Markx, Gerard H ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Capacitance; Dielectric spectroscopy; Dielectrophoresis; Permittivity; Stress; Toxicity; Viability

Indexed keywords

CAPACITANCE; CAPACITANCE MEASUREMENT; CELL DEATH; CELL MEMBRANES; CERAMIC CAPACITORS; CYTOLOGY; DIELECTRIC DEVICES; DIELECTRIC PROPERTIES; DIELECTRIC SPECTROSCOPY; ELECTRIC CONDUCTIVITY; ELECTROPHORESIS;

COMPARATIVE STUDIES; DIELECTROPHORESIS; ENVIRONMENTAL STRESSES; MEMBRANE CONDUCTIVITIES; MEMBRANE PERMEABILITIES; VIABILITY; VIABLE CELLS; YEAST CELLS;

CELLS;

ARTICLE; CELL DEATH; CELL MEMBRANE CONDUCTANCE; CELL MEMBRANE PERMEABILITY; CELL VIABILITY; COMPARATIVE STUDY; ELECTRIC CAPACITANCE; ELECTROPHORESIS; NONHUMAN; SPECTROSCOPY; YEAST CELL;

EID: 55949121863     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2008.09.006     Document Type: Article

References (32)

1. Patel P.M., and Markx G.H. Dielectric measurement of cell death. Enz Microb Technol 43 (2008) 463-470
2. Kell D.B., Kaprelyants A.S., Weichart D.H., Harwood C.R., and Barer M.R. Viability and activity in readily culturable bacteria: a review and discussion of the practical issues. Antonie van Leeuwenhoek 73 (1998) 169-187
3. Schwartz S.M., and Bennett M.R. Death by any other name. Am J Pathol 147 (1995) 229-234
4. Ratanachoo K., Gascoyne P.R.C., and Ruchirawat M. Detection of cellular responses to toxicants by dielectrophoresis. Biochim Biophys Acta - Biomembr 1564 (2002) 449-458
5. Markx G.H., Talary M.S., and Pethig R. Separation of viable and non-viable yeast using dielectrophoresis. J Biotechnol 32 (1994) 29-37
6. Davey C.L., Markx G.H., and Kell D.B. On the dielectric method of monitoring cellular viability. Pure Appl Chem 65 (1993) 1921-1926
7. Dalton C., Goater A.D., Drysdale J., and Pethig R. Parasite viability by electrorotation. Coll. Surf A - Phys Eng Asp 195 (2002) 263-268
8. Morita S., Umakoshi H., and Kuboi R.J. Characterization and on-line monitoring of cell disruption and lysis using dielectric measurement. Biosci Bioeng 88 (1999) 78-84
9. Gascoyne P.R.C., Becker F.F., and Wang X.B. Numerical analysis of the influence of experimental conditions on the accuracy of dielectric parameters derived from electrorotation measurements. Bioelectrochem Bioenerg 36 (1995) 115-125
10. Carvell J.P., and Dowd J.E. On-line measurements and control of viable cell density in cell culture manufacturing processes using radio-frequency impedance. Cytotechnology 50 (2006) 35-48
11. Asami K. Characterization of biological cells by dielectric spectroscopy. J Non Cryst Solids 305 (2002) 268-277
12. Kell D.B., Markx G.H., Davey C.L., and Todd R.W. Real-time monitoring of cellular biomass: methods and applications. Trends Anal Chem 9 (1990) 190-194
13. Pohl H.A. Dielectrophoresis (1978), Cambridge University Press, Cambridge
14. Markx G.H., and Pethig R. Dielectrophoretic separation of cells: continuous separation. Biotechnol Bioeng 45 (1995) 337-343
15. Oblak J., Krizaj D., Amon S., Macek-Lebar A., and Miklavcic D. Feasibility study for cell electroporation detection and separation by means of dielectrophoresis. Bioelectrochemistry 71 (2007) 164-171
16. Stoicheva N.G., Davey C.L., Markx G.H., and Kell D.B. Dielectric spectroscopy: a rapid method for the determination of solvent biocompatibility during biotransformations. Biocatalysis 2 (1989) 245-255
17. Tileva F.P., Yotova L.K., and Markx G.H. Dielectric measurement of the resistance of Trichosporon cutaneum against toxic chemicals. African Journal of Microbiology Research 1 (2007) 117-120
18. Markx G.H., and Kell D.B. The use of dielectric permittivity for the control of the biomass level during biotransformations of toxic substrates in continuous culture. Biotechnol Prog 11 (1995) 64-70
19. Soares E.V., Vroman A., Mortier J., Rijsbrack K., and Mota M. Carbohydrate carbon sources induce loss of flocculation of an ale-brewing yeast strain. J Appl Microb 96 (2004) 1117-1123
20. Kell D.B., Davey C.L., and Todd R.W. Real-time measurement of cellular biomass using dielectric spectroscopy. In: Greenshields R.N. (Ed). Biotechnology International. 2nd Edition (1992), Grosvenor Press, London 294-297
21. Harris C.M., Todd R.W., Bungard S.J., Lovitt R.W., Morrs J.G., and Kell D.B. Dielectric permittivity of microbial suspensions at radio frequencies: a novel method for the real-time estimation of microbial biomass. Enz Microb Technol 9 (1987) 181-186
22. Ferreira A.P., Vieira L.M., Cardoso J.P., and Menezes J.C. Evaluation of a new annular capacitance probe for biomass monitoring in industrial pilot-scale fermentations. J Biotechnol 116 (2005) 403-409
23. Flores-Rodriguez N., and Markx G.H. Flow-through devices for the AC electrokinetic construction of microstructured materials. J Micromech Microeng 16 (2006) 349-355
24. In: Sinclair C.G., Kristiansen B., and Bu'Lock J.D. (Eds). Fermentation Kinetics and Modelling (1987), Open University Press, Milton Keynes
25. Seeman P. Membrane actions of anesthetics and tranquillizers. Pharmacol Rev 24 (1972) 583-655
26. Asami K. Effects of membrane disruption on dielectric properties of biological cells. J Phys D: Appl Phys 39 (2006) 4656-4663
27. Ansorge S., Esteban G., and Schmid G. On-line monitoring of infected Sf-9 insect cell cultures by scanning permittivity measurements and comparison with off-line biovolume measurements. Cytotechnology 55 (2007) 115-124
28. Cannizzaro C., Gugerli R., Marison I., and Stockar U. On-line biomass monitoring of CHO perfusion culture with scanning dielectric spectroscopy. Biotechnol Bioeng 84 (2003) 597-610
29. Carvell J., Bhat A., Patel P.M., and Markx G.H. Recent developments in using scanning radio-frequency impedance measurements on cell culture processes. Bioprocess J 4 (2007) 24-27
30. Gimsa J., Marszalek P., Loewe U., and Tsong T.Y. Dielectrophoresis and electrorotation of neurospora slime and murine myeloma cells. Biophys J 60 (1991) 749-760
31. Labeed F.H., Coley H.M., and Hughes M.P. Differences in the biophysical properties of membrane and cytoplasm of apoptotic cells revealed using dielectrophoresis. Biochim Biophys Acta -Gen Subj 1760 (2006) 922-929
32. Huang Y., Hölzel R., Pethig R., and Wang X.B. Differences in the AC electrodynamics of viable and non-viable yeast-cells determined through combined dielectrophoresis and electrorotation studies. Phys Med Biol 37 (1992) 1499-1517