Evaluation of a commercial immunoblot, helicoblot 2.1, for diagnosis of Helicobacter pylori infection

Clinical and Vaccine Immunology

Volumn 15, Issue 11, 2008, Pages 1705-1710

  Veijola, Lea ^a,b   Oksanen, Aino ^a   Sipponen, Pentti ^c   Rautelin, Hilpi ^b,d  

^a Herttoniemi Hospital   (Finland)

^b UNIVERSITY OF HELSINKI   (Finland)

^c Biohit Oyj   (Finland)

^d HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

CAGA PROTEIN;

ADULT; AGED; ARTICLE; DIAGNOSTIC VALUE; DIFFERENTIAL DIAGNOSIS; ENZYME IMMUNOASSAY; ERADICATION THERAPY; EVALUATION; FEMALE; FOLLOW UP; GASTROSCOPY; HELICOBACTER INFECTION; HELICOBACTER PYLORI; HUMAN; IMMUNOBLOTTING; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY;

ADULT; AGE FACTORS; AGED; AGED, 80 AND OVER; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BLOTTING, WESTERN; FEMALE; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MALE; MIDDLE AGED; SENSITIVITY AND SPECIFICITY;

EID: 55849147343     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00165-08     Document Type: Article

References (50)

1. Annibale, B., E. Lahner, A. Santucci, D. Vaira, A. Pasquali, C. Severi, R. Mini, N. Figura, and G. Delle Fave. 2007. CagA and VacA are immunoblot markers of past Helicobacter pylori infection in atrophic body gastritis. Helicobacter 12:23-30.
2. Barton, S. G., V. R. Winrow, D. S. Rampton, J. E. Crabtree, I. L. Beales, and J. Calam. 1998. Circulating antibodies to the 60-kDa heat shock protein (hsp) family in patients with Helicobacter pylori infection. Clin. Exp. Immunol. 112:490-494.
3. Dixon, M. F., R. M. Genta, J. H. Yardley, and P. Correa. 1996. Classification and grading of gastritis. The updated Sydney System. International Workshop on the Histopathology of Gastritis, Houston 1994. Am. J. Surg. Pathol. 20:1161-1181.
4. Ekesbo, R., E. Toth, F. T. Fork, M. Held, I. Nilsson, T. Wadstrom, and K. Sjolund. 2006. Chronic Helicobacter pylori infection in a population in southern Sweden analysed by histopathology, immunoblot and ELISA serology. Eur. J. Gastroenterol. Hepatol. 18:589-593.
5. Ekstrom, A. M., M. Held, L. E. Hansson, L. Engstrand, and O. Nyren. 2001. Helicobacter pylori in gastric cancer established by CagA immunoblot as a marker of past infection. Gastroenterology 121:784-791.
6. Enroth, H., W. Kraaz, T. Rohan, O. Nyren, and L. Engstrand. 2002. Does the method of Helicobacter pylori detection influence the association with gastric cancer risk? Scand. J. Gastroenterol. 37:884-890.
7. Eurohepygast Study Group. 2002. Risk factors for atrophic chronic gastritis in a European population: results of the Eurohepygast study. Gut 50:779-785.
8. Figueiredo, C., W. Quint, N. Nouhan, H. van den Munckhof, P. Herbrink, J. Scherpenisse, W. de Boer, P. Schneeberger, G. Perez-Perez, M. J. Blaser, and L. J. van Doorn. 2001. Assessment of Helicobacter pylori vacA and cagA genotypes and host serological response. J. Clin. Microbiol. 39:1339-1344.
9. Fusconi, M., D. Vaira, M. Menegatti, S. Farinelli, N. Figura, J. Holton, C. Ricci, R. Corinaldesi, and M. Miglioli. 1999. Anti-CagA reactivity in Helicobacter pylori-negative subjects: a comparison of three different methods. Dig. Dis. Sci. 44:1691-1695.
10. Held, M., L. Engstrand, L. E. Hansson, R. Bergstrom, T. Wadstrom, and O. Nyren. 2004. Is the association between Helicobacter pylori and gastric cancer confined to CagA-positive strains? Helicobacter 9:271-277.
11. Ho, B., and B. J. Marshall. 2000. Accurate diagnosis of Helicobacter pylori. Serologic testing. Gastroenterol. Clin. N. Am. 29:853-862.
12. Hoang, T. T., A. S. Rehnberg, T. U. Wheeldon, C. Bengtsson, D. C. Phung, R. Befrits, M. Sorberg, and M. Granstrom. 2006. Comparison of the performance of serological kits for Helicobacter pylori infection with European and Asian study populations. Clin. Microbiol. Infect. 12:1112-1117.
13. Kamiya, S., H. Yamaguchi, T. Osaki, and H. Taguchi. 1998. A virulence factor of Helicobacter pylori: role of heat shock protein in mucosal inflammation after H. pylori infection. J. Clin. Gastroenterol. 27:S35-S39.
14. Kist, M., S. Strobel, T. Kirchner, and H. G. Dammann. 1999. Impact of ELISA and immunoblot as diagnostic tools one year after eradication of Helicobacter pylori in a multicentre treatment study. FEMS Immunol. Med. Microbiol. 24:239-242.
15. Kivi, M., Y. Tindberg, C. Bengtsson, L. Engstrand, and M. Granstrom. 2005. Assessment of the cag pathogenicity island status of Helicobacter pylori infections with serology and PCR. Clin. Microbiol. Infect. 11:66-68.
16. Kosunen, T. U., A. Aromaa, P. Knekt, A. Salomaa, H. Rautelin, P. Lohi, and O. P. Heinonen. 1997. Helicobacter antibodies in 1973 and 1994 in the adult population of Vammala, Finland. Epidemiol. Infect. 119:29-34.
17. Kusters, J. G., A. H. van Vliet, and E. J. Kuipers. 2006. Pathogenesis of Helicobacter pylori infection. Clin. Microbiol. Rev. 19:449-490.
18. Lu, C. Y., C. H. Kuo, Y. C. Lo, H. Y. Chuang, Y. C. Yang, I. C. Wu, F. J. Yu, Y. C. Lee, C. M. Jan, W. M. Wang, and D. C. Wu. 2005. The best method of detecting prior Helicobacter pylori infection. World J. Gastroenterol. 11:5672-5676.
19. Luthra, G. K., A. R. DiNuzzo, W. K. Gourley, and S. E. Crowe. 1998. Comparison of biopsy and serological methods of diagnosis of Helicobacter pylori infection and the potential role of antibiotics. Am. J. Gastroenterol. 93:1291-1296.
20. Macchia, G., A. Massone, D. Burroni, A. Covacci, S. Censini, and R. Rappuoli. 1993. The Hsp60 protein of Helicobacter pylori: structure and immune response in patients with gastroduodenal diseases. Mol. Microbiol. 9:645-652.
21. Malfertheiner, P., L. C. Fry, and K. Monkemuller. 2006. Can gastric cancer be prevented by Helicobacter pylori eradication? Baillieres Best Pract. Res. Clin. Gastroenterol. 20:709-719.
22. Malfertheiner, P., F. Megraud, C. O'Morain, F. Bazzoli, E. ElOmar, D. Graham, R. Hunt, T. Rokkas, N. Vakil, E. J. Kuipers, et al. 2007. Current concepts in the management of Helicobacter pylori infection: the Maastricht III Consensus Report. Gut 56:772-781.
23. Megraud, F., and P. Lehours. 2007. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin. Microbiol. Rev. 20:280-322.
24. Mini, R., B. Annibale, E. Lahner, G. Bernardini, N. Figura, and A. Santucci. 2006. Western blotting of total lysate of Helicobacter pylori in cases of atrophic body gastritis. Clin. Chem. 52:220-226.
25. Monteiro, L., A. de Mascarel, A. M. Sarrasqueta, B. Bergey, C. Barberis, P. Talby, D. Roux, L. Shouler, D. Goldfain, H. Lamouliatte, and F. Megraud. 2001. Diagnosis of Helicobacter pylori infection: noninvasive methods compared to invasive methods and evaluation of two new tests. Am. J. Gastroenterol. 96:353-358.
26. Ogunc, D., R. Artan, G. Ongut, T. Gelen, D. Colak, L. Donmez, and M. Gultekin. 2003. Evaluation of a Western blot technique (Helicoblot 2.1) for the diagnosis of Helicobacter pylori infection in children. Pathology 35:157-160.
27. Oksanen, A., L. Veijola, P. Sipponen, K. O. Schauman, and H. Rautelin. 1998. Evaluation of Pyloriset Screen, a rapid whole-blood diagnostic test for Helicobacter pylori infection. J. Clin. Microbiol. 36:955-957.
28. Oleastro, M., R. Matos, J. Cabral, R. Barros, A. I. Lopes, P. Ramalho, and L. Monteiro. 2002. Evaluation of a Western blot test, Helico blot 2.1, in the diagnosis of Helicobacter pylori infection in a pediatric population. Helicobacter 7:210-215.
29. Palli, D., G. Masala, G. Del Giudice, M. Plebani, D. Basso, D. Berti, M. E Numans, M. Ceroti, P. H. Peeters, H. B. de Mesquita, F. L. Buchner, F. Clavel-Chapelon, M. C. Boutron-Ruault, V. Krogh, C. Saieva, P. Vineis, S. Panico, R. Tumino, O. Nyren, H. Siman, G. Berglund, G. Hallmans, M. J. Sanchez, N. Larranaga, A. Barricarte, C. Navarro, J. R. Quiros, T. Key, N. Allen, S. Bingham, K. T. Khaw, H. Boeing, C. Weikert, J. Linseisen, G. Nagel, K. Overvad, R. W. Thomsen, A. Tjonneland, A. Olsen, A. Trichoupoulou, D. Trichopoulos, A. Arvaniti, G. Pera, R. Kaaks, M. Jenab, P. Ferrari, G. Nesi, F. Carneiro, E. Riboli, and C. A. Gonzalez. 2007. CagA+ Helicobacter pylori infection and gastric cancer risk in the EPIC-EURGAST study. Int. J. Cancer 120:859-867.
30. Park, C. Y., Y. K. Cho, T. Kodama, H. M. El-Zimaity, M. S. Osato, D. Y. Graham, and Y. Yamaoka. 2002. New serological assay for detection of putative Helicobacter pylori virulence factors. J. Clin. Microbiol. 40:4753-4756.
31. Parkin, D. M. 2006. The global health burden of infection-associated cancers in the year 2002. Int. J. Cancer 118:3030-3044.
32. Raymond, J., C. Sauvestre, N. Kalach, M. Bergeret, and C. Dupont. 2000. Immunoblotting and serology for diagnosis of Helicobacter pylori infection in children. Pediatr. Infect. Dis. J. 19:118-121.
33. Rehnberg-Laiho, L., H. Rautelin, P. Koskela, S. Sarna, E. Pukkala, A. Aromaa, P. Knekt, and T. U. Kosunen. 2001. Decreasing prevalence of helicobacter antibodies in Finland, with reference to the decreasing incidence of gastric cancer. Epidemiol. Infect. 126:37-42.
34. Rocha, G. A., A. M. Oliveira, D. M. Queiroz, A. S. Carvalho, and A. M. Nogueira. 2000. Immunoblot analysis of humoral immune response to Helicobacter pylori in children with and without duodenal ulcer. J. Clin. Microbiol. 38:1777-1781.
35. Rudi, J., C. Kolb, M. Maiwald, I. Zuna, A. von Herbay, P. R. Galle, and W. Stremmel. 1997. Serum antibodies against Helicobacter pylori proteins VacA and CagA are associated with increased risk for gastric adenocarcinoma. Dig. Dis. Sci. 42:1652-1659.
36. Sande, N., M. Nikulin, I. Nilsson, T. Wadstrom, F. Laxen, M. Harkonen, O. Suovaniemi, and P. Sipponen. 2001. Increased risk of developing atrophic gastritis in patients infected with CagA+ Helicobacter pylori. Scand. J. Gastroenterol. 36:928-933.
37. Shimoyama, T., S. Fukuda, F. Nakasato, T. Yoshimura, T. Mikami, and A. Munakata. 2005. Relation of CagA seropositivity to cagPAI phenotype and histological grade of gastritis in patients with Helicobacter pylori infection. World J. Gastroenterol. 11:3751-3755.
38. Siman, J. H., L. Engstrand, G. Berglund, C. H. Floren, and A. Forsgren. 2005. Evaluation of western blot CagA seropositivity in Helicobacter pylori-seropositive and -seronegative subjects. Clin. Diagn. Lab. Immunol. 12:304-309.
39. Sipponen, P., M. Kekki, J. Haapakoski, T. Ihamaki, and M. Siurala. 1985. Gastric cancer risk in chronic atrophic gastritis: statistical calculations of cross-sectional data. Int. J. Cancer 35:173-177.
40. Sorberg, M., L. Engstrand, M. Strom, K. A. Jonsson, H. Jorbeck, and M. Granstrom. 1997. The diagnostic value of enzyme immunoassay and immunoblot in monitoring eradication of Helicobacter pylori. Scand. J. Infect. Dis. 29:147-151.
41. Suriani, R., I. Venturini, M. Colozza, F. Bona, E. Cardesi, and D. Mazzucco. 2002. Helicobacter pylori antibodies (CagA and VacA) detection. The link between cancer and infection. Minerva Gastroenterol. Dietol. 48:159-164.
42. Veijola, L., E. Myllyluoma, R. Korpela, and H. Rautelin. 2005. Stool antigen tests in the diagnosis of Helicobacter pylori infection before and after eradication therapy. World J. Gastroenterol. 11:7340-7344.
43. Veijola, L., A. Oksanen, A. Linnala, P. Sipponen, and H. Rautelin. 2007. Persisting chronic gastritis and elevated Helicobacter pylori antibodies after successful eradication therapy. Helicobacter 12:605-608.
44. Vilaichone, R. K., V. Mahachai, C. Kositchaiwat, D. Y. Graham, and Y. Yamaoka. 2003. Relation between seroreactivity to low-molecular-weight Helicobacter pylori-specific antigens and disease presentation. Clin. Diagn. Lab. Immunol. 10:1025-1028.
45. Vorobjova, T., O. Ananieva, H. Maaroos, P. Sipponen, K. Villako, M. Utt, I. Nilsson, T. Wadstrom, and R. Uibo. 2001. Seropositivity to Helicobacter pylori heat shock protein 60 is strongly associated with intensity of chronic inflammation, particularly in antrum mucosa: an extension of an 18-year follow-up study of chronic gastritis in Saaremaa, Estonia. FEMS Immunol. Med. Microbiol. 30:143-149.
46. Yamaguchi, H., T. Osaki, N. Kurihara, H. Taguchi, and S. Kamiya. 1999. Reactivity of monoclonal antibody to HSP60 homologue of Helicobacter pylori with human gastric epithelial cells and induction of IL-8 from these cells by purified H. pylori HSP60. J. Gastroenterol. 34:1-5.
47. Yamaoka, Y., T. Kodama, D. Y. Graham, and K. Kashima. 1998. Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains. J. Clin. Microbiol. 36:3433-3434.
48. Yamaoka, Y., T. Kodama, K. Kashima, and D. Y. Graham. 1999. Antibody against Helicobacter pylori CagA and VacA and the risk for gastric cancer. J. Clin. Pathol. 52:215-218.
49. Ye, W., M. Held, H. Enroth, W. Kraaz, L. Engstrand, and O. Nyren. 2005. Histology and culture results among subjects with antibodies to CagA but no evidence of Helicobacter pylori infection with IgG ELISA. Scand. J. Gastroenterol. 40:312-318.
50. Ye, W., M. Held, J. Lagergren, L. Engstrand, W. J. Blot, J. K. McLaughlin, and O. Nyren. 2004. Helicobacter pylori infection and gastric atrophy: risk of adenocarcinoma and squamous-cell carcinoma of the esophagus and adenocarcinoma of the gastric cardia. J. Natl. Cancer Inst. 96:388-396.