Molecular detection of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' infection in cats by direct PCR using whole blood without DNA extraction

Journal of Veterinary Medical Science

Volumn 70, Issue 10, 2008, Pages 1095-1099

  Watanabe, Masashi ^a   Hisasue, Masaharu ^a   Souma, Takehisa ^b   Ohshiro, Shuichi ^c   Yamada, Takatsugu ^a   Tsuchiya, Ryo ^a  

^a AZABU UNIVERSITY   (Japan)

^b Maru P LifeTech Co Ltd   (Japan)

^c Yanbaru Animal Hospital   (Japan)

Author keywords

Blood; Feline; Hemoplasma

Indexed keywords

BACTERIAL DNA;

ANEMIA; ANIMAL; ANIMAL DISEASE; ARTICLE; BLOOD; CAT; CAT DISEASE; CHEMISTRY; GENETICS; HEMATOCRIT; ISOLATION AND PURIFICATION; MICROBIOLOGY; MYCOPLASMA; MYCOPLASMOSIS; POLYMERASE CHAIN REACTION;

ANEMIA; ANIMALS; CAT DISEASES; CATS; DNA, BACTERIAL; HEMATOCRIT; MYCOPLASMA; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION;

CANDIDATUS MYCOPLASMA HAEMOMINUTUM; FELIDAE; MYCOPLASMA; MYCOPLASMA HAEMOFELIS;

EID: 55749111038     PISSN: 09167250     EISSN: 13477439     Source Type: Journal    
DOI: 10.1292/jvms.70.1095     Document Type: Article

References (16)

1. Al-Soud, W.A. and Radstrom, P. 2001. Purification and characterization of PCR-inhibitory components in blood cells. J. Clin. Microbiol. 39: 485-493.
2. Barnes, W. 1994. PCR amplification of up to 35-kb DNA with high fidelity and high yield from ? bacteriophage templates. Proc. Natl. Acad. Sci. 91: 2216-2220.
3. Burckhardt, J. 1994. Amplification of DNA from whole blood. PCR Methods Appli. 3: 239-243.
4. Cheng, S., Chang, S-Y. and Respess, R. 1994. Long PCR. Nature 369: 684-685.
5. Foley, J.E., Harrus, S., Poland, A., Chomel, B. and Pedersen, N. C. 1998. Molecular, clinical, and pathologic comparison of two distinct strains of Haemobartonella felis in domestic cats. Am. J. Vet. Res. 59: 1581-1588.
6. Fujihara, M., Watanabe, M., Yamada, T. and Harasawa, R. 2007. Occurrence of 'Candidatus Mycoplasma turicensis' infection in domestic cats in Japan. J. Vet. Med. Sci. 69: 1061-1063.
7. Inokuma, H., Taroura, S., Okuda, M., Hisasue, M., Itamoto, K., Une, S., Nakaichi, M. and Taura, Y. 2004. Molecular survey of Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' infection in cats in Yamaguchi and surrounding areas. J. Vet. Med. Sci. 66: 1017-1020.
8. Jensen, W.A., Lappin, M.R, Kamkar, S. and Reagan, W.J. 2001. Use of a polymerase chain reaction assay to detect and differentiate two strains of Haemobartonella felis infection in naturally infected cats. Am. J. Vet. Res. 62: 604-608.
9. Kunkel, T. A. and Soni, A. 1988. Exonucleolytic proofreading enhances the fidelity of DNA synthesis by chick embryo DNA polymerase-?. J. Biol. Chem. 263: 4450-4459.
10. McCusker, J., Dawson, M. T., Noone, D., Gannon, F. and Smith, T. 1992. Improved method for direct PCR amplification from whole blood. Nucleic Acids Res. 20: 6747.
11. Mercier, B., Gaucher, C., Feugeas, O. and Mazurier, C. 1990. Direct PCR from whole blood, without DNA extraction. Nuclic Acids Res. 18: 5908.
12. Messick, J. B. 2004. Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet. Clin. Pathol. 33: 2-13.
13. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press; E.10-E.15.
14. Tasker, S., Helps, C.R., Dav, M.J. Gruffydd-Jones, T.J. and Harbour, D.A. 2003. Use of real-time PCR to detect and quantify Mycoplasma haemofelis and 'Candidatus Mycoplasma haemominutum' DNA. J. Clin. Microbiol. 41: 439-441.
15. Watanabe, M., Hisasue, M., Hashizaki, K., Furuichi, M., Ogata, M., Hisamatsu, S., Ogi, E., Hasegawa, M., Tsuchiya, R. and Yamada, T. 2003. Molecular detection and characterization of Haemobartonella felis in domestic cats in Japan employing sequence-specific polymerase chain reaction (SS-PCR). J. Vet. Med. Sci. 65: 1111-1114.
16. Willi, B., Boretti, F. S., Cattori, V., Tasker, S., Meli, M. L., Reusch, C., Lutz, H. and Hofmann-Lehmann, R. 2005. Identification, molecular characterization, and experimental transmission of a new hemoplasma isolate from a cat with hemolytic anemia in Switzerland. J. Clin. Microbiol. 43: 2581-2585.