Single cell analysis of B lymphocytes from Wegener's granulomatosis: B cell receptors display affinity maturation within the granulomatous lesions

Clinical and Experimental Immunology

Volumn 154, Issue 3, 2008, Pages 339-345

  Voswinkel, J ^a   Assmann, G ^a   Held, G ^a   Pitann, S ^b,c   Gross, W L ^b,c   Holl Ulrich, K ^b   Herlyn, K ^b,c   Mueller, A ^b,c  

^a SAARLAND UNIVERSITY   (Germany)

^b UNIVERSITY OF LÜBECK   (Germany)

^c Rheumaklinik Bad Bramstedt   (Germany)

Author keywords

B lymphocyte; Immunoglobulins; Laser microdissection; PCR; Wegener's granulomatosis

Indexed keywords

AUTOANTIBODY; B LYMPHOCYTE RECEPTOR; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; MYELOBLASTIN; NEUTROPHIL CYTOPLASMIC ANTIBODY;

ARTICLE; B LYMPHOCYTE; BINDING AFFINITY; CELL FUNCTION; CELL MATURATION; GENE MUTATION; GENETIC ANALYSIS; HUMAN; HUMAN TISSUE; IMMUNOGLOBULIN HEAVY CHAIN GENE; IMMUNOGLOBULIN LIGHT CHAIN GENE; PRIORITY JOURNAL; WEGENER GRANULOMATOSIS;

ADULT; AGED; B-LYMPHOCYTES; CELL DIFFERENTIATION; COMPLEMENTARITY DETERMINING REGIONS; GENES, IMMUNOGLOBULIN HEAVY CHAIN; GENES, IMMUNOGLOBULIN LIGHT CHAIN; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MICRODISSECTION; MIDDLE AGED; MUTATION; NOSE; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, B-CELL; WEGENER GRANULOMATOSIS;

EID: 55449110759     PISSN: 00099104     EISSN: 13652249     Source Type: Journal    
DOI: 10.1111/j.1365-2249.2008.03775.x     Document Type: Article

References (23)

1. Kendall PL, Yu G, Woodward EJ, Thomas JW. Tertiary lymphoid structures in the pancreas promote selection of B lymphocytes in autoimmune diabetes. J Immunol 2007 178 : 5643 51.
2. Müller A, Holl-Ulrich K, Lamprecht P, Gross WL. Germinal centre-like structures in Wegener's granuloma: the morphological basis for autoimmunity? Rheumatology 2008 47 : 1111 3.
3. Jennette JC, Falk RJ, Andrassy K et al. Nomenclature of systemic vasculitides. Proposal of an international consensus conference. Arthritis Rheum 1994 37 : 187 92.
4. Schönermarck U, Lamprecht P, Csernok E, Gross WL. Prevalence and spectrum of rheumatic diseases associated with proteinase 3-antineutrophil cytoplasmic antibodies (ANCA) and myeloperoxidase-ANCA. Rheumatology 2001 40 : 178 84.
5. Falk RJ, Terrell RS, Charles LA, Jennette JC. Anti-neutrophil cytoplasmic autoantibodies induce neutrophils to degranulate and produce oxygen radicals in vitro. Proc Natl Acad Sci 1990 87 : 4115 19.
6. Pfister H, Ollert M, Froehlich LF et al. Antineutrophil cytoplasmic autoantibodies against the murine homolog of proteinase 3 (Wegener autoantigen) are pathogenic in vivo. Blood 2004 104 : 1411 18.
7. Reinhold-Keller E, Beuge N, Latza U et al. An interdisciplinary approach to the care of patients with Wegener's granulomatosis. Arthritis Rheum 2000 43 : 1021 32.
8. Jayne D. Update on the European Vasculitis Study Group trials. Curr Opin Rheumatol 2001 13 : 48 55.
9. Voswinkel J, Müller A, Krämer JA et al. B lymphocyte maturation in Wegener's granulomatosis: a comparative analysis of VH genes from endonasal lesions. Ann Rheum Dis 2006 65 : 859 64.
10. Krämer JA, Müller A, Herlyn K et al. B lymphocyte differentiation in granulomatous tissues of the lung and the nasal mucosa in Wegener's granulomatosis: origin of anti-neutrophil cytoplasmic antibody formation? Z Rheumatol 2007 66 : 421 9.
11. + effector memory T-cells and expression of NKG2D-ligand MIC in granulomatous lesions in Wegener's granulomatosis. Clin Immunol 2008 127 : 144 50.
12. Golbin JM, Specks U Targeting B lymphocytes as therapy for ANCA-associated vasculitis. Rheum Dis Clin North Am 2007 33 : 741 54.
13. Sims GP, Shiono H, Willcox N, Stott DI. Somatic hypermutation and selection of B cells in thymic germinal centers responding to acetylcholine receptor in myasthenia gravis. J Immunol 2001 167 : 1935 44.
14. Fink L, Kwapiszewska G, Wilhelm J, Bohle RM. Laser-microdissection for cell type- and compartment-specific analyses on genomic and proteomic level. Exp Toxicol Pathol 2006 57 : 25 9.
15. Küppers R. Molecular single cell PCR analysis of rearranged immunoglobulin genes as a tool to determine the clonal composition of normal and malignant human B cells. Meth. Mol. Biol 2004 271 : 225 38.
16. Leavitt RY, Fauci AS, Bloch DA et al. The American College of Rheumatology 1990 criteria for the classification of Wegener's granulomatosis. Arthritis Rheum 1190 33 : 1101 7.
17. Bräuninger A, Küppers R, Spieker T et al. Molecular analysis of single B cells from T-cell-rich B-cell lymphoma shows the derivation of the tumor cells from mutating germinal center B cells and exemplifies means by which immunoglobulin genes are modified in germinal center B cells. Blood 1999 8 : 2679 87.
18. Chang B, Casali P. The CDR1 sequences of a major proportion of human germline Ig VH genes are inherently susceptible to amino acid replacement. Immunol Today 1994 15 : 367 73.
19. Klein U, Goossens T, Fischer M et al. Somatic hypermutation in normal and transformed human B cells. Immunol Rev 1998 162 : 261 80.
20. Brezinschek HP, Brezinschek RI, Lipsky PE. Analysis of the heavy chain repertoire of human peripheral B cells using single-cell polymerase chain reaction. J Immunol 1995 155 : 190 202.
21. Lossos IS, Tibshirani R, Narasimhan B, Levy R. The inference of antigen selection on Ig genes. J Immunol 2000 165 : 5122 6.
22. Pugh-Bernard AE, Silverman GJ, Cappione AJ et al. Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance. J Clin Invest 2001 108 : 1061 70.
23. Seitz S, Schneider CK, Malotka J et al. Reconstitution of paired T cell receptor α- and β-chains from microdissected single cells of human inflammatory tissues. Proc Natl Acad Sci USA 2006 103 : 12057 62.