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Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression
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Ali IK, Ehrenkaufer GM, Hackney JA, Singh U. Growth of the protozoan parasite Entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression. BMC Genomics 2007; 8:7.
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Ehrenkaufer GM, Eichinger DJ, Singh U. Trichostatin A effects on gene expression in the protozoan parasite Entamoeba histolytica. BMC Genomics 2007; 8:216.
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This is the first study to transcriptionally profile the cyst stage of E. histolytica, parasites allowing identification of a number of genes specific to this developmental stage and new insights into stage conversion
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Ehrenkaufer GM, Haque R, Hackney JA, et al. Identification of developmentally regulated genes in Entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite. Cell Microbiol 2007; 9:1426-1444. This is the first study to transcriptionally profile the cyst stage of E. histolytica, parasites allowing identification of a number of genes specific to this developmental stage and new insights into stage conversion.
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Bracha R, Nuchamowitz Y, Anbar M, Mirelman D. Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica. PLoS Pathog 2006; 2:e48. This study further elucidates mechanisms behind the newly identified epigenetic gene silencing mechanisms identified in E. histolytica and shows that it is possible to silence multiple genes permanently in this special parasite strain. An important tool for gene regulation in E. histolytica.
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Bracha R, Nuchamowitz Y, Anbar M, Mirelman D. Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica. PLoS Pathog 2006; 2:e48. This study further elucidates mechanisms behind the newly identified epigenetic gene silencing mechanisms identified in E. histolytica and shows that it is possible to silence multiple genes permanently in this special parasite strain. An important tool for gene regulation in E. histolytica.
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Boettner DR, Huston CD, Linford AS, et al. Entamoeba histolytica phagocytosis of human erythrocytes involves PATMK, a member of the transmembrane kinase family. PLoS Pathog 2008; 4:e8. A phagocytosis associated transmembrane kinase (PATMK) is identified as the potential receptor involved in phagocytosis of human erythrocytes, a receptor on the parasite surface that has eluded research until now.
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Boettner DR, Huston CD, Linford AS, et al. Entamoeba histolytica phagocytosis of human erythrocytes involves PATMK, a member of the transmembrane kinase family. PLoS Pathog 2008; 4:e8. A phagocytosis associated transmembrane kinase (PATMK) is identified as the potential receptor involved in phagocytosis of human erythrocytes, a receptor on the parasite surface that has eluded research until now.
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16
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37749001014
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Picazarri K, Nakada-Tsukui K, Nozaki T. Autophagy during proliferation and encystation in the protozoan parasite Entamoeba invadens. Infect Immun 2008; 76:278-288.
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MacFarlane RC, Singh U. Identification of an Entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity. Eukaryot Cell 2007; 6:2139-2146.
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The lysine- and glutamic acid-rich protein KERP1 plays a role in Entamoeba histolytica liver abscess pathogenesis
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KERP1 is identified as a novel virulence factor, based on expression at higher levels in liver derived parasites than those in culture. Downregulation of this protein shows that it may contribute to liver abscess formation
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Santi-Rocca J, Weber C, Guigon G, et al. The lysine- and glutamic acid-rich protein KERP1 plays a role in Entamoeba histolytica liver abscess pathogenesis. Cell Microbiol 2008; 10:202-217. KERP1 is identified as a novel virulence factor, based on expression at higher levels in liver derived parasites than those in culture. Downregulation of this protein shows that it may contribute to liver abscess formation.
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Cell Microbiol
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Santi-Rocca, J.1
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21
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The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress
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Sen A, Chatterjee NS, Akbar MA, et al. The 29-kilodalton thiol-dependent peroxidase of Entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress. Eukaryot Cell 2007; 6:664-673.
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Sen, A.1
Chatterjee, N.S.2
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Urban S. Rhomboid proteins: conserved membrane proteases with divergent biological functions. Genes Dev 2006; 20:3054-3068.
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An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion
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This study identifies the first rhomboid protease in E. histolytica and shows that it targets the heavy subunit of the amebic surface lectin and may play a role in immune evasion
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Baxt LA, Baker RP, Singh U, Urban S. An Entamoeba histolytica rhomboid protease with atypical specificity cleaves a surface lectin involved in phagocytosis and immune evasion. Genes Dev 2008; 22:1636-1646. This study identifies the first rhomboid protease in E. histolytica and shows that it targets the heavy subunit of the amebic surface lectin and may play a role in immune evasion.
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Baxt, L.A.1
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24
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Melendez-Lopez SG, Herdman S, Hirata K, et al. Use of recombinant Entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model. Eukaryot Cell 2007; 6:1130-1136.
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Melendez-Lopez, S.G.1
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Expression and function of a family of transmembrane kinases from the protozoan parasite Entamoeba histolytica
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Eh Klp5 is a divergent member of the kinesin 5 family that regulates genome content and microtubular assembly in Entamoeba histolytica
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Dastidar PG, Majumder S, Lohia A. Eh Klp5 is a divergent member of the kinesin 5 family that regulates genome content and microtubular assembly in Entamoeba histolytica. Cell Microbiol 2007; 9:316-328.
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Wender N, Villalobo E, Mirelman D. EhLimA, a novel LIM protein, localizes to the plasmamembrane in Entamoeba histolytica. Eukaryot Cell 2007; 6:1646-1655.
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Wender N, Villalobo E, Mirelman D. EhLimA, a novel LIM protein, localizes to the plasmamembrane in Entamoeba histolytica. Eukaryot Cell 2007; 6:1646-1655.
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Mitra BN, Saito-Nakano Y, Nakada-Tsukui K, et al. Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica. Cell Microbiol 2007; 9:2112-2125. This study identifies a Rab11b as the protein involved in vesicle trafficking, which releases cysteine proteases from the trophozoite.
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Mitra BN, Saito-Nakano Y, Nakada-Tsukui K, et al. Rab11B small GTPase regulates secretion of cysteine proteases in the enteric protozoan parasite Entamoeba histolytica. Cell Microbiol 2007; 9:2112-2125. This study identifies a Rab11b as the protein involved in vesicle trafficking, which releases cysteine proteases from the trophozoite.
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Two Rab7 isotypes, EhRab7A and EhRab7B, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite Entamoeba histolytica
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Saito-Nakano Y, Mitra BN, Nakada-Tsukui K, et al. Two Rab7 isotypes, EhRab7A and EhRab7B, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite Entamoeba histolytica. Cell Microbiol 2007; 9:1796-1808.
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Induction of monocyte chemotactic protein 1 in colonic epithelial cells by Entamoeba histolytica is mediated via the phosphatidylinositol 3-kinase/p65 pathway
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Kammanadiminti SJ, Dey I, Chadee K. Induction of monocyte chemotactic protein 1 in colonic epithelial cells by Entamoeba histolytica is mediated via the phosphatidylinositol 3-kinase/p65 pathway. Infect Immun 2007; 75:1765-1770.
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This study shows that it is possible to protect against E. histolytica infection in gerbils and mice using a combination adjuvant and Gal-lectin vaccine
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Ivory CP, Chadee K. Intranasal immunization with Gal-inhibitable lectin plus an adjuvant of CpG oligodeoxynucleotides protects against Entamoeba histolytica challenge. Infect Immun 2007; 75:4917-4922. This study shows that it is possible to protect against E. histolytica infection in gerbils and mice using a combination adjuvant and Gal-lectin vaccine.
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