Combinatorial chemopreventive effect of Azadirachta indica and Ocimum sanctum on oxidant-antioxidant status, cell proliferation, apoptosis and angiogenesis in a rat forestomach carcinogenesis model

Singapore Medical Journal

Volumn 49, Issue 10, 2008, Pages 814-822

  Manikandan, P ^a   Vidjaya Letchoumy, P ^a   Prathiba, D ^b   Nagini, Siddavaram ^a  

^a ANNAMALAI UNIVERSITY   (India)

^b SRI RAMACHANDRA UNIVERSITY   (India)

Author keywords

Antioxidants; Apoptosis; Azadirachta indica; Cell proliferation; Chemoprevention; Gastric cancer; Ocimum sanctum; Oxidant antioxidant status; Rat forestomach carcinogenesis model

Indexed keywords

AZADIRACHTA INDICA EXTRACT; CASPASE 3; CATALASE; CYCLINE; CYTOCHROME C; CYTOKERATIN; GLUTATHIONE; GLUTATHIONE TRANSFERASE; METHYLNITRONITROSOGUANIDINE; OCIMUM SANCTUM EXTRACT; PROTEIN BAX; PROTEIN BCL 2; SUPEROXIDE DISMUTASE; VASCULOTROPIN;

ANGIOGENESIS; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; CANCER COMBINATION CHEMOTHERAPY; CELL PROLIFERATION; CHEMOPROPHYLAXIS; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVITY; FORESTOMACH; LIPID PEROXIDATION; MALE; NONHUMAN; PLANT LEAF; PROTEIN DEGRADATION; PROTEIN EXPRESSION; RAT; STOMACH CANCER;

ANIMALS; ANTIOXIDANTS; APOPTOSIS; AZADIRACHTA; DISEASE MODELS, ANIMAL; MALE; METHYLNITRONITROSOGUANIDINE; NEOVASCULARIZATION, PATHOLOGIC; OCIMUM; OXIDANTS; PHYTOTHERAPY; PLANT EXTRACTS; RATS; RATS, WISTAR; STOMACH NEOPLASMS;

EID: 54949104987     PISSN: 00375675     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. Crew KD, Neugut AI. Epidemiology of gastric cancer. World J Gastroenterol 2006; 12:354-62.
2. Zheng L, Wang L, Ajani J, Xie K. Molecular basis of gastric cancer development and progression. Gastric Cancer 2004; 7:61-77.
3. Arivazhagan S, Balasenthil S, Nagini S. Attenuation by garlic and neem leaf extracts of sodium chloride enhanced gastric carcinogenesis induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) in Wistar rats. GI Cancer 2001; 3:375-82.
4. Velmurugan B, Mani A, Nagini S. Combination of S-allylcysteine and lycopene induces apoptosis by modulating Bcl-2, Bax, Bim and caspases during experimental gastric carcinogenesis. Eur J Cancer Prev 2005; 14:387-93.
5. Subapriya R, Nagini S. Ethanolic neem leaf extract protects against MNNG-induced gastric carcinogenesis in Wistar rats. Asian Pacific J Cancer Prev 2003; 4:215-23.
6. Park EJ, Pezzuto JM. Botanicals in cancer chemoprevention. Cancer Metastasis Rev 2002; 21:231-55.
7. Subapriya R, and Nagini S. Medicinal properties of neem leaves: A review. Curr Med Chem Anticancer Agents 2005; 5:149-56.
8. Gupta SK, Prakash J, Srivastava S. Validation of traditional claim of tulsi, Ocimum sanctum Linn. as a medicinal plant. Indian J Exp Biol 2002; 40: 765-73.
9. Manikandan P, Murugan RS, Abbas H, Abraham SK, Nagini S. Ocimum sanctum Linn. (Holy Basil) ethanolic leaf extract protects against 7,12-dimethylbenz(a) anthracene-induced genotoxicity, oxidative stress, and imbalance in xenobiotic-metabolizing enzymes. J Med Food 2007; 10:495-502.
10. Garg GP, Nigam SK, Ogle CW. The gastric antiulcer effects of the leaves of the neem tree. Planta Med 1993; 59:215-7.
11. Goel RK, Sairam K, Dorababu M, Prabhu T, Rao CHV. Effect of standardized extract of Ocimum sanctum Linn. on gastric mucosal offensive and defensive factors. Indian J Exp Biol 2005; 43: 715-21.
12. Febry W, Okema P, Ansory R. Activity of East African medicinal plants against Helicobactor pylori. Chemotherapy 1996; 42:315-7.
13. Manikandan P, Vidjaya Letchoumy P, Prathiba D, Nagini S. Proliferation, angiogenesis and apoptosis-associated proteins are molecular targets for chemoprevention of MNNG-induced gastric carcinogenesis by ethanolic Ocimum sanctum leaf extract. Singapore Med J 2007; 48:645-51.
14. Wang J, Guo LL, Wang YY. [Study on combination components and effectiveness of Chinese traditional herbal formulas]. Zhongguo Zhong Yao Za Zhi 2006; 31:5-9. Chinese.
15. Saravanan K, Nagarajan B. Genotoxicity assessment in vivo in gastric carcinogenesis. Med Sci Res 1993; 21:119-21.
16. Ohkawa H, Ohisi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-8.
17. Jiang ZY, Hunt JV, Wolff SP. Detection of lipid hydroperoxides using Fox method. Anal Biochem 1992; 202:384-9.
18. Rao KS, Recknagel RO. Early onset of lipid peroxidation in rat liver after carbon tetrachloride administration. Exp Mol Pathol 1968; 9:271-8.
19. Levine RL, Garland D, Oliver CN, et al. Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 1990; 186:464-78.
20. Oberley LW, Spitz DR. Assay of superoxide dismutase activity in tumor tissue. Methods Enzymol 1984; 105:457-64.
21. Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972; 47:389-394.
22. Anderson ME. Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 1985: 113:548-55.
23. Srivastava SK, Beutler E. Accurate measurement of oxidized glutathione content of human, rabbit, and rat red blood cells. Anal Biochem 1968; 25:70-6.
24. Rotruck JT, Pope AL, Ganther HE, et al. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973; 179:588-90.
25. Lawrence A, Burk RF. Glutathione peroxidase activity in selenium deficient rat liver. Biochem Biophys Res Commun 1976; 71:952-8.
26. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 49:7130-9.
27. Fiala S, Fiala AE, Dixon B. Gamma-glutamyl transpeptidase in transplantable, chemically induced rat hepatomas and "spontaneous" mouse hepatomas. J Natl Cancer Inst 1972; 48:1393-401.
28. Carlberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985; 113:484-90.
29. Lowry OH, Rosebrough NJ, Farr AL, Randall RY. Protein measurement with the Folin-phenol reagent. J Biol Chem 1951; 193:265-75.
30. Chajes V, Sattler W, Stranzl A, Kostner GM Influence of n-3 fatty acids on the growth of human breast cancer cells in vitro: relationship to peroxides and vitamin-E. Breast Cancer Res Treat 1995; 34:199-212.
31. Poot M, Teubert H, Rabinovitch PS, Kavanagh TJ. De novo synthesis of glutathione is required for both entry into and progression through the cell cycle. J Cell Physiol 1995; 163:555-60.
32. Jónsson ZO, Hindges R, Hübscher U. Regulation of DNA replication and repair proteins through interaction with the front side of proliferating cell nuclear antigen. EMBO J 1998; 17:2412-25.
33. Maga G, Hubscher U. Proliferating cell nuclear antigen (PCNA): a dancer with many partners, J Cell Sci 2003; 116:3051-60.
34. Zhao X, Ean Y, Shen J, Wu Y, Yin Z. Human glutathione S-transferase P1 suppresses MEKK1-mediated apoptosis by regulating MEKK1 kinase activity in HEK293 cells. Mol Cell 2006; 21:395-400.
35. Prakash J. Gupta SK, Singh N, Kochupillai V, Gupta YR. Antiproliferative and chemopreventive activity of Ocimum sanctum Linn. Int J Med Biol Environ 1999; 27: 165-72.
36. Uma Devi P. Radioprotective, anticarcinogenic and antioxidant properties of the Indian holy basil, Ocimum sanctum (tulsi). Indian J Exp Biol 2001; 39:185-90.
37. Kumar S, Suresh PK, Vijayababu MR, Arunkumar A, Arunakaran J. Anticancer effects of ethanolic neem leaf extract on prostate cancer cell line (PC-3). J Ethnopharmacol, 2005; 105:246-50.
38. Cao Y, Cao R. Angiogenesis inhibited by drinking tea. Nature 1999; 398:381.
39. Chandra Mohan KVP, Devaraj H, Prathiba D, Hara Y, Nagini S. Antiproliferative and apoptosis inducing effect of lactoferrin and black tea polyphenol combination on hamster buccal pouch carcinogenesis. Biochim Biophys Acta 2006; 1760:1536-44.
40. Skommer J, Wlodkowic D, Deptala A. Larger than life: Mitochondria and the Bcl-2 family. Leuk Res 2007; 31: 277-86.
41. Thatte U, Bagadey S, Dahanukar S. Modulation of programmed cell death by medicinal plants. Cell Mol Biol 2000; 46:199-214.