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Because of the low solubility of the crystalline trimethylsilylated derivative in the mixture of ethanol and H2O, THF (the ratio of THF/ethanol was 1:1) was added to obtain a homogeneous solution. The final molar ratios were trimethylsilylated derivative/ethanol (containing 10 of [D6]ethanol)/THF/H2O/HCl=1:36:26:18:0.06
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