A simple homogeneous scintillation proximity assay for acyl-coenzyme A:diacylglycerol acyltransferase

Analytical Biochemistry

Volumn 383, Issue 2, 2008, Pages 144-150

  Seethala, Ramakrishna ^a   Peterson, Tara ^a   Dong, Jessica ^a   Chu, Ching Hsuen ^a   Chen, Luping ^a   Golla, Rajasree ^a   Ma, Zhengping ^a   Panemangalore, Reshma ^a   Lawrence, R Michael ^a   Cheng, Dong ^a  

^a BRISTOL MYERS SQUIBB   (United States)

Author keywords

DGAT; Diacylglycerol; High throughput screening; Monoacylglycerol; Obesity; Oleoyl CoA; Stearoyl CoA; Triacylglycerol

Indexed keywords

COENZYMES; LIPIDS; NUTRITION; SCINTILLATION; THIN LAYER CHROMATOGRAPHY;

DGAT; DIACYLGLYCEROL; HIGH THROUGHPUT SCREENING; MONOACYLGLYCEROL; OBESITY; OLEOYL-COA; STEAROYL-COA; TRIACYLGLYCEROLS;

GLYCEROL;

2 OLEOYLGLYCEROL; BOVINE SERUM ALBUMIN; DIACYLGLYCEROL; DIACYLGLYCEROL ACYLTRANSFERASE; DIMETHYL SULFOXIDE; GLYCEROL OLEATE; POLYLYSINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL BINDING; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SYNTHESIS; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; SCINTILLATION PROXIMITY ASSAY; SIGNAL TRANSDUCTION; THIN LAYER CHROMATOGRAPHY;

EID: 54449099903     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.09.013     Document Type: Article

References (21)

1. Bell R.M., and Coleman R.A. Enzymes of glycerolipid synthesis in eukaryotes. Annu. Rev. Biochem. 49 (1980) 459-487
2. Levy E. The 1991 Borden Award Lecture: selected aspects of intraluminal and intracellular phases of intestinal fat absorption. Can. J. Physiol. Pharmacol. 70 (1992) 413-419
3. Cases S., Smith S.J., Zheng Y.W., Myers H.M., Lear S.R., Sande E., Novak S., Collins C., Welch C.B., Lusis A.J., Erickson S.K., and Farese Jr. R.V. Identification of a gene encoding an acyl-CoA:diacylglycerol acyltransferase a key enzyme in triacylglycerol synthesis. Proc. Natl. Acad. Sci. USA 95 (1998) 13018-13023
4. Cases S., Stone S.J., Zhou P., Yen E., Tow B., Lardizabal K.D., Voelker T., and Farese Jr. R.V. Cloning of and related family members DGAT2 a second mammalian diacylglycerol acyltransferase. J. Biol. Chem. 276 (2001) 38870-38876
5. Chang C.C., Huh H.Y., Cadigan K.M., and Chang T.Y. Molecular cloning and functional expression of human acyl-coenzyme A:cholesterol acyltransferase cDNA in mutant Chinese hamster ovary cells. J. Biol. Chem. 268 (1993) 20747-20755
6. Smith S.J., Cases S., Jensen D.R., Chen H.C., Sande E., Tow B., Sanan D.A., Raber J., Eckel R.H., and Farese Jr. R.V. Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking DGAT. Nat. Genet. 25 (2000) 87-90
7. Chen H.C., Smith S.J., Ladha Z., Jensen D.R., Ferreira L.D., Pulawa L.K., McGuire J.G., Pitas R.E., Eckel R.H., and Farese Jr. R.V. Increased insulin and leptin sensitivity in mice lacking acyl CoA:diacylglycerol acyltransferase 1. J. Clin. Invest. 109 (2002) 1049-1055
8. Stone S.J., Myers H.M., Watkins S.M., Brown B.E., Feingold K.R., Elias P.M., and Farese Jr. R.V. Lipopenia and skin barrier abnormalities in DGAT2-deficient mice. J. Biol. Chem. 279 (2004) 11767-11776
9. Choi C.S., Savage D.B., Kulkarni A., Yu X.X., Liu Z.X., Morino K., Kim S., Distefano A., Samuel V.T., Neschen S., Zhang D., Wang A., Zhang X.M., Khan M., Cline G.W., Pandey S.K., Geisler J.G., Bhanot S., Monia B.P., and Shulman G.I. Suppression of diacylglycerol acyltransferase-2 (DGAT2) but not DGAT1 with antisense oligonucleotides reverses diet-induced hepatic steatosis and insulin resistance. J. Biol. Chem. 282 (2007) 22678-22688
10. Meegalla R.L., Billheimer J.T., and Cheng D. Concerted elevation of acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) activity through independent stimulation of mRNA expression of DGAT1 and DGAT2 by carbohydrate and insulin. Biochem. Biophys. Res. Commun. 298 (2002) 317-323
11. Yen C.L., Monetti M., Burri B.J., and Farese Jr. R.V. The triacylglycerol synthesis enzyme DGAT1 also catalyzes the synthesis of diacylglycerols, waxes, and retinyl esters. J. Lipid Res. 46 (2005) 1502-1511
12. Orland M.D., Anwar K., Cromley D., Chu C.H., Chen L., Billheimer J.T., Hussain M.M., and Cheng D. Acyl coenzyme A dependent retinol esterification by acyl coenzyme A: diacylglycerol acyltransferase 1. Biochim. Biophys. Acta 1737 (2005) 76-82
13. Yen C.L., Stone S.J., Cases S., Zhou P., and Farese Jr. R.V. Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase. Proc. Natl. Acad. Sci. USA 99 (2002) 8512-8517
14. Cao J., Burn P., and Shi Y. Properties of the mouse intestinal acyl-CoA:monoacylglycerol acyltransferase, MGAT2. J. Biol. Chem. 278 (2003) 25657-25663
15. Yen C.L., and Farese Jr. R.V. MGAT2, a monoacylglycerol acyltransferase expressed in the small intestine. J. Biol. Chem. 278 (2003) 18532-18537
16. Cheng D., Nelson T.C., Chen J., Walker S.G., Wardwell-Swanson J., Meegalla R., Taub R., Billheimer J.T., Ramaker M., and Feder J.N. Identification of acyl coenzyme A:monoacylglycerol acyltransferase 3, an intestinal specific enzyme implicated in dietary fat absorption. J. Biol. Chem. 278 (2003) 13611-13614
17. Cao J., Hawkins E., Brozinick J., Liu X., Zhang H., Burn P., and Shi Y. A predominant role of acyl-CoA:monoacylglycerol acyltransferase-2 in dietary fat absorption implicated by tissue distribution, subcellular localization, and up-regulation by high fat diet. J. Biol. Chem. 279 (2004) 18878-18886
18. Smith J.L., Lear S.R., and Erickson S.K. Developmental expression of elements of hepatic cholesterol metabolism in the rat. J. Lipid Res. 36 (1995) 641-652
19. Cheng D., Meegalla R.L., He B., Cromley D.A., Billheimer J.T., and Young P.R. Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric protein. Biochem. J. 359 (2001) 707-714
20. Coleman R.A. Diacylglycerol acyltransferase and monoacylglycerol acyltransferase from liver and intestine. Methods Enzymol. 209 (1992) 98-104
21. Zhang J.H., Chung T.D., and Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4 (1999) 67-73