The role of the active site-coordinating cysteine residues in the maturation of the H2-sensing [NiFe] hydrogenase from Ralstonia eutropha H16

Archives of Microbiology

Volumn 182, Issue 2-3, 2004, Pages 138-146

  Winter, Gordon ^a   Buhrke, Thorsten ^a   Jones, Anne K ^a   Friedrich, Bärbel ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

NiFe hydrogenase; H2 sensor; Metal center assembly; Nickel metabolism; Ralstonia eutropha

Indexed keywords

CHAPERONE; HYDROGENASE; NICKEL IRON HYDROGENASE; PROTEIN HOXC; PROTEIN HYPC; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME BINDING; GENE MUTATION; IMMUNOBLOTTING; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RALSTONIA EUTROPHA H16; RESIDUE ANALYSIS; WAUTERSIA EUTROPHA;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; CUPRIAVIDUS NECATOR; CYSTEINE; HYDROGENASE; IMMUNOBLOTTING; NICKEL; PROTEIN INTERACTION MAPPING; PROTEIN SUBUNITS;

NEGIBACTERIA; RALSTONIA; WAUTERSIA EUTROPHA;

EID: 5444225517     PISSN: 03028933     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00203-004-0680-6     Document Type: Article

References (44)

1. Bernhard M, Schwartz E, Rietdorf J, Friedrich B (1996) The Alcaligenes eutrophus membrane-bound hydrogenase gene locus encodes functions involved in maturation and electron transport coupling. J Bacteriol 178:4522-4529
2. Bernhard M, Benelli B, Hochkoeppler A, Zannoni D, Friedrich B (1997) Functional and structural role of the cytochrome b subunit of the membrane-bound hydrogenase complex of Alcaligenes eutrophus H16. Eur J Biochem 248:179-186
3. 2 sensor of Ralstonia eutropha: biochemical characteristics, spectroscopic properties, and its interaction with a histidine protein kinase. J Biol Chem 276:15592-15597
4. Black LK, Fu C, Maier RJ (1994) Sequence and characterization of hupU and hupV genes of Bradyrhizobium japonicum encoding a possible nickel-sensing complex involved in hydrogenase expression. J Bacteriol 176:7102-7106
5. Blokesch M, Paschos A, Theodoratou E, Bauer A, Hube M, Huth S, Böck A (2002) Metal insertion into NiFe-hydrogenases. Biochem Soc Trans 30:674-680
6. 2-sensing hydrogenase from Ralstonia eutropha in its reduced state by HYSCORE and ENDOR spectroscopy. J Am Chem Soc 125:13075-13083
7. 2-sensing [NiFe] hydrogenase of Ralstonia eutropha. J Bacteriol 183:7087-7093
8. 2 sensor of Ralstonia eutropha: biochemical and spectroscopic analysis of mutant proteins modified at a conserved glutamine residue close to the [NiFe] active site. J Biol Inorg Chem 7:897-908
9. 2-sensing complex of Ralstonia eutropha: interaction between a regulatory [NiFe] hydrogenase and a histidine protein kinase. Mol Microbiol 51:1677-1689
10. 17O ENDOR detection of a solvent-derived Ni-(OH(x))-Fe bridge that is lost upon activation of the hydrogenase from Desulfovibrio gigas. J Am Chem Soc 124:281-286
11. Chen B, Przybyla AE (1994) An efficient site-directed mutagenesis method based on PCR. Biotechniques 17:657-659
12. Drapal N, Böck A (1998) Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation. Biochemistry 37:2941-2948
13. Elsen S, Colbeau A, Chabert J, Vignais PM (1996) The hupTUV operon is involved in negative control of hydrogenase synthesis in Rhodobacter capsulatus. J Bacteriol 178:5174-5181
14. 2 sensor HupUV and the histidine kinase HupT controls HupSL hydrogenase synthesis in Rhodobacter capsulatus. J Bacteriol 185:7111-7119
15. Fodor BD, Kovács AT, Csáki R, Hunyadi-Gulyás E, Klement E, Maróti G, Mészáros LS, Medzihradszky KF, Rákhely G, Kovács KL (2004) Modular broad-host-range expression vectors for single-protein and protein complex purification. Appl Environ Microbiol 70:712-721
16. Happe RP, Roseboom W, Pierik AJ, Albracht SP, Bagley KA (1997) Biological activation of hydrogen. Nature 385:126
17. Haumann M, Porthun A, Buhrke T, Liebisch P, Meyer-Klaucke W, Friedrich B, Dau H (2003) Hydrogen-induced structural changes at the nickel site of the regulatory [NiFe] hydrogenase from Ralstonia eutropha detected by X-ray absorption spectroscopy. Biochemistry 42:11004-11015
18. Higuchi Y, Yagi T, Yasuoka N (1997) Unusual ligand structure in Ni-Fe active center and an additional Mg site in hydrogenase revealed by high resolution X-ray structure analysis. Structure 5:1671-1680
19. 2 sensor of Ralstonia eutropha is a member of the subclass of regulatory [NiFe]-hydrogenases. J Bacteriol 182:2716-2724
20. Kovács KL, Fodor B, Kovács AT, Csanádi G, Maróti G, Balogh J, Arvani S, Rákhely G (2002) Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina. Int J Hydrogen Energy 27:1463-1469
21. 2 sensing in Alcaligenes eutrophus. Proc Natl Acad Sci USA 95:12474-12479
22. Lenz O, Schwartz E, Dernedde J, Eitinger M, Friedrich B (1994) The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation. J Bacteriol 176:4385-4393
23. Lenz O, Bernhard M, Buhrke T, Schwartz E, Friedrich B (2002) The hydrogen-sensing apparatus in Ralstonia eutropha. J Mol Microbiol Biotechnol 4:255-262
24. Lowry OH, Rosebroug NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275
25. Magalon A, Böck A (2000) Analysis of the HypC-HycE complex, a key intermediate in the assembly of the metal center of the Escherichia coli hydrogenase 3. J Biol Chem 275:21114-21120
26. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor
27. 2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus. Biochemistry 38:14330-14337
28. 3. J Biol Inorg Chem 6:63-81
29. Montet Y, Amara P, Volbeda A, Vernede X, Hatchikian EC, Field MJ, Frey M, Fontecilla-Camps JC (1997) Gas access to the active site of Ni-Fe hydrogenases probed by X-ray crystallography and molecular dynamics. Nat Struct Biol 4:523-526
30. Pierik AJ, Schmelz M, Lenz O, Friedrich B, Albracht SPJ (1998) Characterization of the active site of a hydrogen sensor from Alcaligenes eutrophus. FEBS Lett 438:231-235
31. Pohlmann A, Cramm R, Schmelz K, Friedrich B (2000) A novel NO-responding regulator controls the reduction of nitric oxide in Ralstonia eutropha. Mol Microbiol 38:626-638
32. Schink B, Schlegel HG (1979) The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification and biochemical properties. Biochem Biophys Acta 567:315-324
33. Schneider K, Schlegel HG (1976) Purification and properties of the soluble hydrogenase from Alcaligenes eutrophus H16. Biochim Biophys Acta 452:66-80
34. Schwartz E, Friedrich B (2001) A physical map of the megaplasmid pHG1, one of three genomic replicons in Ralstonia eutropha H16. FEMS Microbiol Lett 201:213-219
35. 2-metabolizing prokaryotes. In: Dworkin M, Schleifer KH, Stackebrandt E (eds) The prokaryotes: an evolving electronic resource for the microbial community, 3rd edn. release 3.14, Springer, New York
36. Schwartz E, Gerischer U, Friedrich B (1998) Transcriptional regulation of Alcaligenes eutrophus hydrogenase genes. J Bacteriol 180:3197-3204
37. 2-based lithoautotrophy and anaerobiosis. J Mol Biol 332:369-383
38. Simon R, Priefer U, Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Bio/Technology 1:717-743
39. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350-4357
40. Tran-Betcke A, Warnecke U, Böcker C, Zaborosch C, Friedrich B (1990) Cloning and nucleotide sequences of the genes for the subunits of NAD-reducing hydrogenase of Alcaligenes eutrophus H16. J Bacteriol 172:2920-2929
41. Vignais PM, Billoud B, Meyer J (2001) Classification and phylogeny of hydrogenases. FEMS Microbiol Rev 25:455-501
42. Volbeda A, Charon M-H, Piras C, Hatchikan EC, Frey M, Fontecilla-Camps JC (1995) Crystal structure of the nickel-iron hydrogenase from Desulfovibrio gigas. Nature 373:580-587
43. Yanisch-Perron C, Viera J, Messing J (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119
44. Zimmer D, Schwartz E, Tran-Betcke A, Gewinner P, Friedrich B (1995) Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA. J Bacteriol 177:2373-2380