Assembly of α-hemolysin on A431 cells leads to clustering of Caveolin-1

Biochemical and Biophysical Research Communications

Volumn 324, Issue 3, 2004, Pages 1124-1129

  Vijayvargia, Ravi ^a   Kaur, Surinder ^a   Sangha, Navneet ^a   Sahasrabuddhe, Anagh A ^a   Surolia, Ira ^a   Shouche, Yogesh ^a   Krishnasastry, Musti V ^a  

^a NATIONAL CENTRE FOR CELL SCIENCE   (India)

Author keywords

Hemolysin; Caveolin 1 clustering; Heptameric assembly; Lipid rafts; Scaffolding domain

Indexed keywords

ALPHA HEMOLYSIN; CAVEOLIN 1; HEMOLYSIN; OLIGOMER; STAPHYLOCOCCUS TOXIN; UNCLASSIFIED DRUG; WATER;

AMINO ACID COMPOSITION; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CARCINOMA CELL; CELL COMMUNICATION; CONFORMATION; CONTROLLED STUDY; FEMALE; HUMAN; HUMAN CELL; HYDROPHOBICITY; MEMBRANE; MUTANT; NONHUMAN; PERMEABILITY; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN ISOLATION; SOLUBILITY; ULTRACENTRIFUGATION; UTERUS CARCINOMA;

AMINO ACID MOTIFS; BACTERIAL TOXINS; CAVEOLIN 1; CAVEOLINS; CELL LINE, TUMOR; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEMOLYSIN PROTEINS; HUMANS; IMMUNOPRECIPITATION; KINETICS; MEMBRANE MICRODOMAINS; METHIONINE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MUTATION; PEPTIDES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RHODAMINES; TIME FACTORS;

EID: 5444222951     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.09.165     Document Type: Article

References (22)

1. S. Bhakdi, and J. Tranum-Jensen Alpha-toxin of Staphylococcus aureus Microbiol. Rev. 55 1991 733 751
2. A. Valeva, I. Walev, M. Pinkernell, B. Walker, H. Bayley, M. Palmer, and S. Bhakdi Transmembrane beta-barrel of staphylococcal alpha-toxin forms in sensitive but not in resistant cells Proc. Natl. Acad. Sci. USA 94 1997 11607 11611
3. M. Watanabe, T. Tomita, and T. Yasuda Membrane-damaging action of staphylococcal alpha-toxin on phospholipid-cholesterol liposomes Biochim. Biophys. Acta 898 1987 257 265
4. T. Tomita, M. Watanabe, and T. Yasuda Influence of membrane fluidity on the assembly of Staphylococcus aureus alpha-toxin, a channel-forming protein, in liposome membrane J. Biol. Chem. 267 1992 13391 13397
5. S. Pany, R. Vijayvargia, and M.V. Krishnasastry Caveolin-1 binding motif of alpha-hemolysin: its role in stability and pore formation Biochem. Biophys. Res. Commun. 322 2004 29 36
6. J. Couet, M. Sargiacomo, and M.P. Lisanti Interaction of a receptor tyrosine kinase, EGF-R, with caveolins. Caveolin binding negatively regulates tyrosine and serine/threonine kinase activities J. Biol. Chem. 272 1997 30429 30438
7. S. Vandana, S. Navneet, K. Surinder, and M.V. Krishnasastry Modulation of EGF receptor autophosphorylation by alpha-hemolysin of Staphylococcus aureus via protein tyrosine phosphatase FEBS Lett. 535 2003 71 76
8. U.K. Laemmli Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 1970 680 685
9. S. Vandana, M. Raje, and M.V. Krishnasastry The role of the amino terminus in the kinetics and assembly of alpha-hemolysin of Staphylococcus aureus J. Biol. Chem. 272 1997 24858 24863
10. M.P. Lisanti, P.E. Scherer, J. Vidugiriene, Z. Tang, A. Hermanowski-Vosatka, Y.H. Tu, R.F. Cook, and M. Sargiacomo Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease J. Cell Biol. 126 1994 111 126
11. S. Li, T. Okamoto, M. Chun, M. Sargiacomo, J.E. Casanova, S.H. Hansen, I. Nishimoto, and M.P. Lisanti Evidence for a regulated interaction between heterotrimeric G proteins and caveolin J. Biol. Chem. 270 1995 15693 15701
12. K.S. Song, S. Li, T. Okamoto, L.A. Quilliam, M. Sargiacomo, and M.P. Lisanti Co-purification and direct interaction of Ras with caveolin, an integral membrane protein of caveolae microdomains. Detergent-free purification of caveolae microdomains J. Biol. Chem. 271 1996 9690 9697
13. H. Huo, X. Guo, S. Hong, M. Jiang, X. Liu, and K. Liao Lipid rafts/caveolae are essential for insulin-like growth factor-1 receptor signaling during 3T3-L1 preadipocyte differentiation induction J. Biol.Chem. 278 2003 11561 11569
14. P. Lazarovici, and K.F. Chan Staphylococcus aureus alpha-toxin. 2. Reduction of epidermal growth factor receptor affinity in PC12 cells Toxicon 25 1987 637 647
15. H. Lee, D. Volonte, F. Galbiati, P. Iyengar, D.M. Lublin, D.B. Bregman, M.T. Wilson, R. Campos-Gonzalez, B. Bouzahzah, R.G. Pestell, P.E. Scherer, and M.P. Lisanti Constitutive and growth factor-regulated phosphorylation of caveolin-1 occurs at the same site (Tyr-14) in vivo: identification of a c-Src/Cav-1/Grb7 signaling cassette Mol. Endocrinol. 14 2000 1750 1775
16. D. Volonte, F. Galbiati, R.G. Pestell, and M.P. Lisanti Cellular stress induces the tyrosine phosphorylation of caveolin-1 (Tyr(14)) via activation of p38 mitogen-activated protein kinase and c-Src kinase. Evidence for caveolae, the actin cytoskeleton, and focal adhesions as mechanical sensors of osmotic stress J. Biol. Chem. 276 2001 8094 8103
17. B. Vecsey-Semjen, R. Mollby, and F.G. van der Goot Partial C-terminal unfolding is required for channel formation by staphylococcal alpha-toxin J. Biol. Chem. 271 1996 8655 8660
18. I. Fernandez, Y. Ying, J. Albanesi, and R.G. Anderson Mechanism of caveolin filament assembly Proc. Natl. Acad. Sci. USA 99 2002 11193 11198
19. A. Arbuzova, L. Wang, J. Wang, G. Hangyas-Mihalyne, D. Murray, B. Honig, and S. McLaughlin Membrane binding of peptides containing both basic and aromatic residues. Experimental studies with peptides corresponding to the scaffolding region of caveolin and the effector region of MARCKS Biochemistry 39 2000 10330 10339
20. L. Song, M.R. Hobaugh, C. Shustak, S. Cheley, H. Bayley, and J.E. Gouaux Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore Science 274 1996 1859 1866
21. A. Valeva, A. Weisser, B. Walker, M. Kehoe, H. Bayley, S. Bhakdi, and M. Palmer Molecular architecture of a toxin pore: a 15-residue sequence lines the transmembrane channel of staphylococcal alpha-toxin EMBO J. 15 1996 1857 1864
22. B. Walker, and H. Bayley Key residues for membrane binding, oligomerization, and pore forming activity of staphylococcal alpha-hemolysin identified by cysteine scanning mutagenesis and targeted chemical modification J. Biol. Chem. 270 1995 23065 23071