A homologous promoterless K-ras cDNA targeting endogenous K-ras expression inhibits human pancreatic cancer cell growth in vitro and in vivo

Cancer Biology and Therapy

Volumn 7, Issue 10, 2008, Pages 1641-1647

  Ren, Xin Yu ^a   Liang, Zhi Yong ^a   Shi, Xiao Hua ^a   Liu, Tong Hua ^a  

^a PEKING UNION MEDICAL COLLEGE HOSPITAL   (China)

Author keywords

Endogenous gene; Gene therapy; Homologous dependant gene silencing; K ras; Pancreatic cancer; Promoterless cDNA; siRNA

Indexed keywords

COMPLEMENTARY DNA; K RAS PROTEIN; PLASMID VECTOR; POLYETHYLENEIMINE; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTINEOPLASTIC ACTIVITY; APOPTOSIS; ARTICLE; CANCER CELL; CANCER GENE THERAPY; CANCER GROWTH; CANCER INHIBITION; CARCINOGENICITY; CELL PROLIFERATION; CONTROLLED STUDY; DOSE TIME EFFECT RELATION; DRUG EFFECT; FEMALE; GENE CONSTRUCT; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; MOLECULAR CLONING; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PANCREAS CANCER; PROMOTER REGION; PROTEIN EXPRESSION; RNA INTERFERENCE; TUMOR VOLUME;

EID: 54349129093     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.7.10.6586     Document Type: Article

References (31)

1. McKenna S and Eatock M. The medical management of pancreatic cancer: A review. Oncologist 2003; 8:149-60.
2. Bardeesy N and DePinho RA. Pancreatic cancer biology and genetics. Nat Rev Cancer 2002; 2:897-909.
3. Aram F. Hezel, Alec C. Kimmelman, Ben Z. Stanger, etc. Genetics and biology of pancreatic ductal adenocarcinoma. Genes & Dev 2006; 20:1218-49.
4. Sharon L. Campbell, Roya Khosravi-Far, Kent L. Rossman, etc. Increasing complexity of Ras signaling. Oncogene 1998; 17:1395-413.
5. Deramaudt T, Rustgi AK. Mutant K-RAS in the initiation of pancreatic cancer. Biochim Biophy Acta 2005; 1756:97-101.
6. Malumbres M and Barbacid M. RAS oncogenes: The first 30 years. Nat Rev Cancer 2003; 3:459-65.
7. Rozenblum E, Schutte M, Goggins M, et al. Tumor-suppressive pathways in pancreatic carcinoma. Cancer Res 1997; 57:1731-4.
8. Patrick S. Moore, Stefania Beghelli, Giuseppe Zamboni and Aldo Scarpa. Genetic abnormalities in pancreatic cancer. Molecular Cancer 2003; 2:7-13.
9. Bos JL. Ras oncogenes in human cancer: a review. Cancer Res 1989; 49:4682-9.
10. Almoguera C, Shibata D, Forrester K, Martin J, Arnheim N and Perucho M. Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes. Cell 1988; 53:549-54.
11. Pasca di Magliano M, Sekine S, Ermilov A, etc. Hedgehog/Ras interactions regulate early stages of pancreatic cancer. Genes Dev 2006; 20:3161-73.
12. Jens Kurreck. siRNA Efficiency: Structure or sequence - that is the question. J Biomed and Biotechnol 2006; 4: 83757-64.
13. Oliver Voinnet, Philippe Vain, et al. Systemic spread of sequence-specific transgene RNA degradation in plants is initiated by localized introduction of ectopic promoterless DNA. Cell 1998; 95:177-87.
14. Palauqui JC, Balzergue S. Activation of systemic silencing by localised introduction of DNA. Curr Biol 1999; 9:59-66.
15. Rutherford G, Tanurdzic M, Hasebe M, Banks JA. A systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes. BMC Plant Biol 2004; 4:6.
16. Kawai-Toyooka, Chihiro Kuramoto, Kyoko Orui, Kentaro Motoyama, Kazuhiro Kikuchi, Takeshi Kanegae, Masamitsu Wada. DNA Interference: a simple and efficient gene-silencing system for high-throughput functional analysis in the fern adiantum. Plant Cell Physiol 2004; 45:1648-57.
17. Mohammad B. Bahramian and Helmut Zarbl. Transcriptional and posttranscriptional silencing of rodent α1(I) collagen by a homologous transcriptionally self-silenced transgene. Mol Cell Biol 1999; 19: 274-83.
18. Muller-Taubenberger A. Application of fluorescent protein tags as reporters in live-cell imaging studies. Methods Mol Biol 2006; 46:229-46.
19. Aoki K, Yoshida T, Sugimura T, Terada M. Liposome-mediated in vivo gene transfer of antisense K-ras construct inhibits pancreatic tumor dissemination in the murine peritoneal cavity. Cancer Res 1995; 55:3810-6.
20. Su Z, Lebedeva IV, Gopalkrishnan RV, Goldstein NI, Stein CA, Reed JC, Dent P and Fisher PB. A combinatorial approach for selectively inducing programmed cell death in human pancreatic cancer cells. Proc Natl Acad Sci USA 2001; 98:10332-7.
21. Jason BF, Shen GL, Shane EH, Rolf AB. Molecular consequences of silencing mutant K-ras in pancreatic cancer cells: justification for K-ras-directed therapy. Mol Cancer Res 2005; 3:413-23.
22. Park CJ, Park SA, Yoon TG, Lee SJ, Yum KW and Kim HJ. Bupivacaine Induces Apoptosis via ROS in the Schwann Cell Line. J Dent Res 2005; 84:852-7.
23. Lebedeva IV, Sarkar D, Su ZZ, Kitada S, Dent P, Stein CA, Reed JC and Fisher PB. Bcl-2 and Bcl-x(L) differentially protect human prostate cancer cells from induction of apoptosis by melanoma differentiation associated gene-7, mda-7/IL-24. Oncogene 2003; 22:8758-73.
24. Dormoy-Raclet V, Markovits J, Malato Y. Unr, a cytoplasmic RNA-binding protein with cold-shockdomains,is involved in control of apoptosis in ES and HuH7 cells. Oncogene 2007; 26:2595-605.
25. Silvia G, Isolda C, Lourdes F, Adela M, et al. K-ras codon 12 mutation induces higher level of resistance to apoptosis and predisposition to anchorage-independent growth than codon 13 mutation or proto-oncogene overexpression. Cancer Res 2000; 60:6750-6.
26. Jason BF, Shen GL, Shane EH, Mishel D, Rolf AB. Molecular consequences of silencing mutant K-ras in pancreatic cancer cells: justification for K-ras-directed therapy. Mol Cancer Res 2005; 3:413-23.
27. Cogoni C. Homology-dependent gene silencing mechanisms in fungi. Annu Rev Microbiol 2001; 55:381-406.
28. Meyer P. Annual. Homology-dependent gene silencing in plants. Review of Plant Physiology and Plant Molecular Biology 1996; 47:23-48.
29. Bahramian MB, Zarbl H. Direct gene quantitation by PCR reveals differential accumulation of ectopic enzyme in Rat-1 cells, v-fos transformants, and revertants. PCR Methods Appl 1994; 4:145-53.
30. Baulcombe DC. Ectopic pairing of homologous DNA and pist-transcriptional gene silencing in transgenic plants. Curr Opin Biotech 1996; 7:173-80.
31. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-strand RNA in caenorhabditis elegans. Nature 1998; 391:806-11.