Reduction of high background staining by heating unfixed mouse skeletal muscle tissue sections allows for detection of thermostable antigens with murine monoclonal antibodies

Journal of Histochemistry and Cytochemistry

Volumn 56, Issue 11, 2008, Pages 969-975

  Mundegar, Rustam R ^a   Franke, Elke ^b   Schäfer, Ralf ^a   Zweyer, Margit ^a   Wernig, Anton ^a  

^a UNIVERSITY OF BONN   (Germany)

^b TaconicArtemis GmbH   (Germany)

Author keywords

Background reduction; Boiling; Double immunofluorescence; Dystrophin; Homologous tissue; Immunohistochemistry; Monoclonal mouse antibodies

Indexed keywords

ANTIGEN; DYSTROPHIN; FLUORESCEIN DERIVATIVE; FLUOROCHROME; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; TRITON X 100;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIGEN DETECTION; ARTICLE; CONTROLLED STUDY; FEMALE; FROZEN SECTION; HEATING; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; IN SITU HYBRIDIZATION; MALE; MOUSE; MUSCLE TISSUE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; SKELETAL MUSCLE; SPECIES; STAINING; TISSUE SECTION; Y CHROMOSOME;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGENS; BUFFERS; DYSTROPHIN; FROZEN SECTIONS; HEAT; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; MICE; MICE, INBRED MDX; MUSCLE, SKELETAL; PHOSPHATES; SODIUM CHLORIDE; SOLUTIONS; Y CHROMOSOME;

MURINAE;

EID: 54349093719     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2008.950105     Document Type: Article

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