A novel multicolor immunofluorescence method using heat treatment

Acta Medica Okayama

Volumn 59, Issue 4, 2005, Pages 145-151

  Suzuki, Takao ^a   Tate, Genshu ^a   Ikeda, Katsuhide ^a   Mitsuya, Toshiyuki ^a  

^a SHOWA UNIVERSITY FUJIGAOKA HOSPITAL   (Japan)

Author keywords

Confocal laser scanning microscope; Heat inactivation; Multicolor immunofluorescence

Indexed keywords

ANTIBODY; ANTIGEN; FLUORESCENT DYE;

ANIMAL; ARTICLE; CHEMISTRY; FLUORESCENT ANTIBODY TECHNIQUE; HEAT; HUMAN; METABOLISM; METHODOLOGY; MOUSE; RABBIT;

ANIMALS; ANTIBODIES; ANTIGENS; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; HEAT; HUMANS; MICE; RABBITS;

ORYCTOLAGUS CUNICULUS;

EID: 25144491689     PISSN: 0386300X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Nakane PK: Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody methods: a study on pituitary glands of the rat. J Histochem Cytochem (1968) 16: 557-560.
2. Wang BL and Larsson LI: Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species. Histochemistry (1985) 83: 47-56.
3. Kolodziejczyk E and Baertschi AJ: Multiple immunolabeling in histology: a new method using thermo-inactivation of immunoglobulins. J Histochem Cytochem (1986) 34: 1725-9.
4. Lewis Carl SA, Gillete-Ferguson I and Ferguson DG: An indirect immunofluorescence procedure for staining the same cryosection with two mouse monoclonal primary antibodies. J Histochem Cytochem (1993) 41: 1273-8.
5. Aoki J, Nanba K, Yamamoto T, Sasaki N and Taniyama K: Multimarker immunostaining method using the hot water treatment. Pathology and clinical medicine (1996) 14: 1533-1536 (in Japanese).
6. Shi SR, Key ME and Kalra KL: Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem (1991) 39: 741-748.
7. Lan HY, Mu W, Nikolic-Paterson DJ and Atkins RC: A novel, simple, reliable, and sensitive method for multiple immunoenzyme staining: use of microwave oven heating to block antibody crossreactivity and retrieve antigens. J Histochem Cytochem (1995) 43: 97-102.
8. Shi SR, Cote RJ, Young LL, Taylor CR: Antigen retrieval immunohistochemistry: practice and development. J Histotechol (1997) 20: 145-154.
9. Ikeda K, Suzuki T, Kishimoto K, Kitamura T, Tate G and Mitsuya T: An immunoenzyme triple-staining method using highly sensitive indirect technique. Igaku Kensa (2003) 52: 944-949 (in Japanese).