Rga2 is a Rho2 GAP that regulates morphogenesis and cell integrity in S. pombe

Molecular Microbiology

Volumn 70, Issue 4, 2008, Pages 867-881

  Villar Tajadura, Ma Antonia ^a   Coll, Pedro M ^a   Madrid, Marisa ^b   Cansado, Jose ^b   Santos, Beatriz ^a   Pérez, Pilar ^a  

^a CSIC   (Spain)

^b UNIVERSITY OF MURCIA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN RGA2; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

ACTIN POLYMERIZATION; ARTICLE; CELL STRUCTURE; CYTOLYSIS; MORPHOGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SCHIZOSACCHAROMYCES POMBE;

ACTINS; CELL POLARITY; CELL WALL; DNA, FUNGAL; GENE DELETION; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; GLUCANS; GTPASE-ACTIVATING PROTEINS; MAP KINASE SIGNALING SYSTEM; MICROSCOPY, FLUORESCENCE; MORPHOGENESIS; RHO GTP-BINDING PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS;

SCHIZOSACCHAROMYCES POMBE;

EID: 54249161497     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2008.06447.x     Document Type: Article

References (47)

1. Arellano, M., Durán, A. Pérez, P. (1996) Rho1 GTPase activates the (1-3) β-D-glucan synthase and is involved in Schizosaccharomyces pombe morphogenesis. EMBO J 15 : 4584 4591.
2. Arellano, M., Durán, A. Pérez, P. (1997) Localization of the Schizosaccharomyces pombe rho1 GTPase and its involvement in the organization of the actin cytoskeleton. J Cell Sci 110 : 2547 2555.
3. Arellano, M., Coll, P.M. Pérez, P. (1999a) Rho GTPases in the control of cell morphology, cell polarity, and actin localization in fission yeast. Microsc Res Tech 47 : 51 60.
4. Arellano, M., Valdivieso, M.H., Calonge, T.M., Coll, P.M., Durán, A. Pérez, P. (1999b) Schizosaccharomyces pombe protein kinase C homologues, pck1p and pck2p, are targets of rho1p and rho2p and differentially regulate cell integrity. J Cell Sci 112 : 3569 3578.
5. Bähler, J., Wu, J.-Q., Longtine, M.S., Shah, N.G., McKenzie, A., Steever, A.B., et al. (1998) Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14 : 943 951.
6. Barba, G., Soto, T., Madrid, M., Nunez, A., Vicente, J., Gacto, M. Cansado, J. (2008) Activation of the cell integrity pathway is channelled through diverse signalling elements in fission yeast. Cell Signal 20 : 748 757.
7. Barthe, C., de Bettignies, G., Louvet, O., Peypouquet, M.F., Morel, C., Doignon, F. Crouzet, M. (1998) First characterization of the gene RGD1 in the yeast Saccharomyces cerevisiae. C R Acad Sci III 321 : 453 462.
8. Bone, N., Millar, J.B.A., Toda, T. Armstrong, J. (1998) Regulated vacuole fusion and fission in Schizosaccharomyces pombe: an osmotic response dependent on MAP kinases. Curr Biol 8 : 135 144.
9. Cabib, E., Drgonova, J. Drgon, T. (1998) Role of small G proteins in yeast cell polarization and wall biosynthesis. Annu Rev Biochem 67 : 307 333.
10. Calonge, T.M., Nakano, K., Arellano, M., Arai, R., Katayama, S., Toda, T., et al. (2000) Schizosaccharomyces pombe Rho2 GTPase regulates the cell wall α-glucan biosynthesis, through the protein kinase Pck2p. Mol Biol Cell 11 : 4393 4401.
11. Calonge, T.M., Arellano, M., Coll, P.M. Pérez, P. (2003) Rga5p is a specific Rho1p GTPase-activating protein that regulates cell integrity in Schizosaccharomyces pombe. Mol Microbiol 47 : 507 518.
12. Chiang, S.H., Hwang, J., Legendre, M., Zhang, M., Kimura, A. Saltiel, A.R. (2003) TCGAP, a multidomain Rho GTPase-activating protein involved in insulin-stimulated glucose transport. EMBO J 22 : 2679 2691.
13. Craven, R.A., Griffiths, D.J., Sheldrick, K.S., Randall, R.E., Hagan, I.M. Carr, A.M. (1998) Vectors for the expression of tagged proteins in Schizosaccharomyces pombe. Gene 221 : 59 68.
14. Das, M., Wiley, D.J., Medina, S., Vincent, H.A., Larrea, M., Oriolo, A. Verde, F. (2007) Regulation of cell diameter, For3p localization, and cell symmetry by fission yeast Rho-GAP Rga4p. Mol Biol Cell 18 : 2090 2101.
15. Etienne-Manneville, S. Hall, A. (2002) Rho GTPases in cell biology. Nature 420 : 629 635.
16. Forsburg, S.L. Sherman, D.A. (1997) General purpose tagging vectors for fission yeast. Gene 191 : 191 195.
17. García, P., Tajadura, V., García, I. Sánchez, Y. (2006) Role of Rho GTPases and Rho-GEFs in the regulation of cell shape and integrity in fission yeast. Yeast 23 : 1031 1043.
18. Hirata, D., Nakano, K., Fukui, M., Takenaka, H., Miyakawa, T. Mabuchi, I. (1998) Genes that cause aberrant cell morphology by overexpression in fission yeast: a role for a small GTP-binding protein Rho2 in cell morphogenesis. J Cell Sci 111 : 149 159.
19. Ito, H., Fukuda, Y., Murata, K. Kimura, A. (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153 : 163 168.
20. Katayama, S., Hirata, D., Arellano, M., Pérez, P. Toda, T. (1999) Fission yeast α-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function. J Cell Biol 144 : 1173 1186.
21. Loewith, R., Hubberstey, A. Young, D. (2000) Skh1, the MEK component of the Mkh1 signalling pathway in Schizosaccharomyces pombe. J Cell Sci 113 : 153 160.
22. Ma, Y., Kuno, T., Kita, A., Asayama, Y. Sugiura, R. (2006) Rho2 is a target of the farnesyltransferase Cpp1 and acts upstream of Pmk1 mitogen-activated protein kinase signalling in fission yeast. Mol Biol Cell 17 : 5028 5037.
23. Madrid, M., Soto, T., Khong, H.K., Franco, A., Vicente, J., Pérez, P., et al. (2006) Stress-induced response, localization, and regulation of the Pmk1 cell integrity pathway in Schizosaccharomyces pombe. J Biol Chem 281 : 2033 2043.
24. Merla, A. Johnson, D.I. (2000) The Cdc42p GTPase is targeted to the site of cell division in the fission yeast Schizosaccharomyces pombe. Int J Cell Biol 79 : 469 477.
25. Moreno, S., Klar, A. Nurse, P. (1991) Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol 194 : 795 823.
26. Nakano, K., Arai, R. Mabuchi, I. (1997) The small GTP-binding protein Rho1 is a multifunctional protein that regulates actin localization, cell polarity, and septum formation in the fission yeast Schizosaccharomyces pombe. Genes Cells 2 : 679 694.
27. Nakano, K., Mutoh, T. Mabuchi, I. (2001) Characterization of GTPase-activating proteins for the function of the Rho-family small GTPases in the fission yeast Schizosaccharomyces pombe. Genes Cells 6 : 1031 1042.
28. + encodes a protein kinase that interacts with Cdc42p and is involved in the control of cell polarity and mating. EMBO J 14 : 5908 5919.
29. Park, H.O. Bi, E. (2007) Central roles of small GTPases in the development of cell polarity in yeast and beyond. Microbiol Mol Biol Rev 71 : 48 96.
30. Pinar, M., Coll, P.M., Rincon, S.A. Pérez, P. (2008) Schizosaccharomyces pombe Pxl1 Is a Paxillin Homologue That Modulates Rho1 Activity and Participates in Cytokinesis. Mol Biol Cell 19 : 1727 1738.
31. Ridley, A.J. (2006) Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol 16 : 522 529.
32. Roux, P.P. Blenis, J. (2004) ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions. Microbiol Mol Biol Rev 68 : 320 344.
33. Sayers, L.G., Katayama, S., Nakano, K., Mellor, H., Mabuchi, I., Toda, T. Parker, P.J. (2000) Rho-dependence of Schizosaccharomyces pombe Pck2. Genes Cells 5 : 17 27.
34. Schwamborn, J.C., Muller, M., Becker, A.H. Puschel, A.W. (2007) Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity. EMBO J 26 : 1410 1422.
35. Self, A.J. Hall, A. (1995) Measurement of intrinsic nucleotide exchange and GTP hydrolysis rates. Methods Enzymol 256 : 67 76.
36. Sengar, A.S., Markley, N.A., Marini, N.J. Young, D. (1997) Mkh1, a MEK kinase required for cell wall integrity and proper response to osmotic and temperature stress in Schizosaccharomyces pombe. Mol Cell Biol 17 : 3508 3519.
37. +, a suppressor of calcineurin deficiency, encodes a novel MAP kinase phosphatase in fission yeast. EMBO J 17 : 140 148.
38. Sugiura, R., Toda, T., Dhut, S., Shuntoh, H. Kuno, T. (1999) The MAPK kinase Pek1 acts as a phosphorylation-dependent molecular switch. Nature 399 : 479 483.
39. Tatebe, H., Nakano, K., Maximo, R. Shiozaki, K. (2008) Pom1 DYRK Regulates Localization of the Rga4 GAP to Ensure Bipolar Activation of Cdc42 in Fission Yeast. Curr Biol 18 : 322 330.
40. Tcherkezian, J. Lamarche-Vane, N. (2007) Current knowledge of the large RhoGAP family of proteins. Biol Cell 99 : 67 86.
41. Tiedje, C., Sakwa, I., Just, U. Höfken, T. (2008) The Rho GDI Rdi1 regulates Rho GTPases by distinct mechanisms. Mol Biol Cell 19 : 2885 2896.
42. Toda, T., Dhut, S., Superti-Furga, G., Gotoh, Y., Nishida, E., Sugiura, R. Kuno, T. (1996) The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homologue which regulates cell integrity and functions co-ordinately with the protein kinase C pathway. Mol Cell Biol 16 : 6752 6764.
43. Wang, H., Tang, X., Liu, J., Trautmann, S., Balasundaram, D., McCollum, D. Balasubramanian, M.K. (2002) The Multiprotein Exocyst Complex Is Essential for Cell Separation in Schizosaccharomyces pombe. Mol Biol Cell 13 : 515 529.
44. Wang, H.R., Zhang, Y., Ozdamar, B., Ogunjimi, A.A., Alexandrova, E., Thomsen, G.H. Wrana, J.L. (2003) Regulation of cell polarity and protrusion formation by targeting RhoA for degradation. Science 302 : 1775 1779.
45. Yang, P., Qyang, Y., Bartholomeusz, G., Zhou, X. Marcus, S. (2003) The novel Rho GTPase-activating protein family protein, Rga8, provides a potential link between Cdc42/p21-activated kinase and Rho signalling pathways in the fission yeast, Schizosaccharomyces pombe. J Biol Chem 278 : 48821 48830.
46. Zaitsevskaya-Carter, T. Cooper, J.A. (1997) Spm1, a stress-activated MAP kinase that regulates morphogenesis in S. pombe. EMBO J 16 : 1318 1331.
47. Zheng, Y., Hart, M.J., Shinjo, K., Evans, T., Bender, A. Cerione, R.A. (1993) Biochemical comparisons of the Saccharomyces cerevisiae Bem2 and Bem3 proteins. Delineation of a limit Cdc42 GTPase-activating protein domain. J Biol Chem 268 : 24629 24634.