The yiaKLX1X2PQRS and ulaABCDEFG gene systems are required for the aerobic utilization of L-ascorbate in Klebsiella pneumoniae strain 13882 with L-ascorbate-6-phosphate as the inducer

Journal of Bacteriology

Volumn 190, Issue 20, 2008, Pages 6615-6624

  Campos, Evangelina ^a   De La Riva, Lucia ^a   Garces, Fernando ^a   Giménez, Rosa ^a   Aguilar, Juan ^a   Baldoma, Laura ^a   Badia, Josefa ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ASCORBIC ACID DERIVATIVE; BACTERIAL PROTEIN; CARBON; CYCLIC AMP; CYCLIC AMP BINDING PROTEIN; IHF PROTEIN; LEVO ASCORBATE 6 PHOSPHATE; OXYGEN; PHOSPHOTRANSFERASE; PROTEIN DERIVATIVE; ULAR PROTEIN; UNCLASSIFIED DRUG; VITAMIN; YIAJ PROTEIN;

AEROBIC METABOLISM; ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; CARBON SOURCE; EXPERIMENT; GEL; GENE EXPRESSION; KLEBSIELLA PNEUMONIAE; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; REGULATORY MECHANISM; REGULON; REPRESSOR GENE;

AEROBIOSIS; ARTIFICIAL GENE FUSION; ASCORBIC ACID; BACTERIAL PROTEINS; BETA-GALACTOSIDASE; DNA, BACTERIAL; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE ORDER; GENES, REPORTER; KLEBSIELLA PNEUMONIAE; MOLECULAR SEQUENCE DATA; OPERON; OXIDATION-REDUCTION; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; REGULON; SEQUENCE ANALYSIS, DNA;

ENTEROBACTERIACEAE; KLEBSIELLA PNEUMONIAE; ULA;

EID: 53849142133     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00815-08     Document Type: Article

References (34)

1. Abee, T., and J. A. Wouters. 1999. Microbial stress response in minimal processing. Int. J. Food Microbiol. 50:65-91.
2. Baillon, M. L. A., A. H. M. van Vliet, J. M. Ketley, C. Constantinidou, and C. W. Penn. 1999. An iron-regulated alkyl hydroperoxide reductase (AhpC) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen Campylobacter jejuni. J. Bacteriol. 181:4798-4804.
3. Boronat, A., and J. Aguilar. 1979. Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme. J. Bacteriol. 140:320-326.
4. Campos, E., J. Aguilar, L. Baldoma, and J. Badia. 2002. The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli. J. Bacteriol. 184:6065-6068.
5. Campos, E., L. Baldoma, J. Aguilar, and J. Badia. 2004. Regulation of expression of the divergent ulaG and ulaABCDEF operon involved in L-ascorbate dissimilation in Escherichia coli. J. Bacteriol. 186:1720-1728.
6. Campos, E., C. Montella, F. Garces, L. Baldoma, J. Aguilar, and J. Badia. 2007. Aerobic L-ascorbate metabolism and associated oxidative stress in Escherichia coli. Microbiology 153:3399-3408.
7. Esselen, W. B., and J. E. Fuller. 1939. The oxidation of ascorbic acid as influenced by intestinal bacteria. J. Bacteriol. 37:501-521.
8. Fuqua, W. C. 1992. And improved chloramphenicol resistance gene cassette for site-directed marker replacement mutagenesis. BioTechniques 12:223-225.
9. Grammann, K., A. Volke, and H. J. Kunte. 2002. New type of osmoregulated solute transporter identified in halophilic members of the Bacteria domain: TRAP transporter TeaABC mediates uptake of ectoine and hydroxyectoine in Halomonas elongata DSM 2581T. J. Bacteriol. 184:3078-3085.
10. Grunstein, M., and D. S. Hogness. 1975. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72:3961-3965.
11. Hancock, D., T. Besser, J. Lejeune, M. Davis, and D. Rice. 2001. The control of VTEC in the animal reservoir. Int. J. Food Microbiol. 66:71-78.
12. Ibañez, E., E. Campos, L. Baldomà, J. Aguilar, and J. Badia. 2000. Regulation of expression of the yiaKLMNOPQRS operon for carbohydrate utilization in Escherichia coli: involvement of the main transcriptional factors. J. Bacteriol. 182:4617-4624.
13. Ibañez, E., R. Gimenez, T. Pedraza, L. Baldoma, J. Aguilar, and J. Badia. 2000. Role of the yiaR and yiaS genes of Escherichia coli in metabolism of endogenously formed L-xylulose. J. Bacteriol. 182:4625-4627.
14. Kelly, D. J., and G. H. Thomas. 2001. The tripartite ATP-independent periplasmic (TRAP) transporters of bacteria and archaea. FEMS Microbiol. Rev. 25:405-424.
15. Liao, M. L., S. Y. Wang, C. Chung, Y. T. Liang, and P. A. Sejb. 1988. Synthesis of L-ascorbate-6-phosphate. Carbohydr. Res. 176:73-77.
16. Liu, Q., and R. A. Bender. 2007. Complex regulation of urease formation from the two promoters of the ure operon of Klebsiella pneumoniae. J. Bacteriol. 189:7593-7599.
17. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-273.
18. Ly, A., J. Henderson, A. Lu, D. E. Culham, and J. M. Wood. 2004. Osmoregulatory systems of Escherichia coli: identification of betaine-carnitinecholine transporter family member BetU and distributions of betU and trkG among pathogenic and nonpathogenic isolates. J. Bacteriol. 186:296-306.
19. Martínez, J., L. Martínez, M. Rosemblueth, J. Silva, and E. Martinez-Romero. 2004. How are gene sequence analyses modifying bacterial taxonomy? The case of Klebsiella. Int. Microbiol. 4:261-268.
20. Nunoshiba, T., E. Hidalgo, C. F. Amabile-Cuevas, and B. Demple. 1992. Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene. J. Bacteriol. 174:6054-6060.
21. Plantinga, T. H., C. van der Does, and A. J. M. Driessen. 2004. Transporter's evolution and carbohydrate metabolic clusters. Trends Microbiol. 12:4.
22. Plantinga, T. H., C. Van Der Does, J. Badia, J. Aguilar, W. N. Konings, and A. J. Driessen. 2004. Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes. Mol. Membr. Biol. 21:51-57.
23. Plantinga, T. H., C. van der Does, D. Tomkiewicz, G. van Keulen, W. N. Konings, and A. J. Driessen. 2005. Deletion of the yiaMNO transporter genes affects the growth characteristics of Escherichia coli K-12. Microbiology 151:1683-1689.
24. Rabus, R., D. L. Jack, D. J. Kelly, and M. H. Saier, Jr. 1999. TRAP transporters: an ancient family of extracytoplasmic solute-receptor- dependent secondary active transporters. Microbiology 145:3431-3445.
25. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
26. Sanchez, J. C., R. Gimenez, A. Schneider, W.-D. Fessner, L. Baldomà, J. Aguilar, and J. Badia. 1994. Activation of a cryptic gene encoding a kinase for L-xylulose opens a new pathway for the utilization of L-lyxose by Escherichia coli. J. Biol. Chem. 269:29665-29669.
27. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53:85-96.
28. Sleator, R. D., and C. Hill. 2001. Bacterial osmoadaptation: the role of osmolytes in bacteria stress and virulence. FEMS Microbiol. Rev. 731:1-23.
29. Thomas, G. H., T. Southworth, M. R. León-Kempis, A. Leech, and D. J. Kelly. 2006. Novel ligands for the extracellular solute receptors of two bacterial TRAP transporters. Microbiology 152:187-198.
30. van Rooijen, R. J., K. J. Dechering, C. Niek, J. Wilmink, and W. M. de Vos. 1993. Lysines 72, 80 and 213 and aspartic acid 210 of the Lactococcus lactis LacR repressor are involved in the response to the inducer tagatose-6-phosphate leading to induction of lac operon expression. Protein Eng. 6:201-206.
31. Volk, W. A., and L. Larsen. 1962. β-L-Gulonic acid as an intermediate in the bacterial metabolism of ascorbic acid. J. Biol. Chem. 237:2454-2457.
32. Yew, W.-S., and J. A. Gerlt. 2002. Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of the yjf-sga and yia-sgb operons. J. Bacteriol. 184:302-306.
33. Young, R. M., and L. H. James. 1942. Action of intestinal microorganisms on ascorbic acid. J. Bacteriol. 44:75-84.
34. Zhang, Z., M. Aboulwafa, M. H. Smith, and M. H. Saier, Jr. 2003. The ascorbate transporter of Escherichia coli. J. Bacteriol. 185:2243-2250.