Viewing membrane-bound molecular umbrellas by parallax analyses

Journal of the American Chemical Society

Volumn 130, Issue 41, 2008, Pages 13771-13777

  Kondo, Masaharu ^a   Mehiri, Mohamed ^a   Regen, Steven L ^a  

^a LEHIGH UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASTROPHYSICS; FLUOROPHORES; GEOMETRICAL OPTICS; LIGHT EMISSION; LIPID BILAYERS; LUMINESCENCE; QUENCHING;

FLUORESCENCE QUENCHERS; FLUORESCENCE QUENCHING; FLUOROPHORE; LIPID BILAYER; MEMBRANE-BOUND; PHOSPHOCHOLINE;

FLUORESCENCE;

1 PALMITOYL 2 OLEOYL SN GLYCERO 3 PHOSPHOTEMPOCHOLINE; 1 PALMITOYL 2 STEAROYL (12 N OXYL 4,4 DIMETHYLOXAZOLIDINE) SN GLYCERO 3 PHOSPHOCHOLINE; 1 PALMITOYL 2 STEAROYL (5 N OXYL 4,4 DIMETHYLOXAZOLIDINE) SN GLYCERO 3 PHOSPHOCHOLINE; PHOSPHORYLCHOLINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL ANALYSIS; HYDROPHILICITY; MEMBRANE BINDING; MEMBRANE STRUCTURE; PHOTOCHEMICAL QUENCHING; SYNTHESIS;

FLUORESCENT DYES; MEMBRANES, ARTIFICIAL; MOLECULAR STRUCTURE; NITRIC OXIDE; PHOSPHOLIPIDS; SOLUBILITY; WATER;

EID: 53849084490     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja804929m     Document Type: Article

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37. In an attempt to obtain further support for a minor subpopulation of deep-lying umbrellas for 1b and 2b, we compared the fluorescence quenching of 1a, 1b, 2a, and 2b by 4-hydroxy Tempo in liposomal dispersions. Since deep-lying molecular umbrellas would be expected to exhibit different quenching behavior by 4-hydroxy Tempo relative to ones present in the aqueous phase and those lying at the surface of liposomes, this might be detectable by discontinuity in a Stern-Volmer plot. Such plots, however, did not reveal any discontinuities (Supporting Information). The absence of a discontinuity in the case of 1b and 2b is presumed to be due to insufficient sensitivity of this approach as compared with the parallax method of analysis.