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K. Si, M. Giustetto, A. Etkin, R. Hsu, A.M. Janisiewicz, M.C. Miniaci, J.H. Kim, H. Zhu, and E.R. Kandel A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia Cell 115 2003 893 904 Previous studies have shown the requirement of rapamycin-dependent local translation and CREB-mediated transcription in the serotonin-induced LTF of Aplysia sensory to motor neuron synapses. Here, the authors show that a single pulse of serotonin, which on its own can only produce short-term plasticity, could induce local synthesis of ApCPEB. Antisense oligonucleotides to ApCPEB mRNA inhibited the late (but not early) phase of LTF, which suggests that its role is in the stabilization and maintenance phase of long-term plasticity.
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K.Y. Miyashiro, A. Beckel-Mitchener, T.P. Purk, K.G. Becker, T. Barret, L. Liu, S. Carbonetto, I.J. Weiler, W.T. Greenough, and J. Eberwine RNA cargoes associating with FMRP reveal deficits in cellular functioning in Fmr1 null mice Neuron 37 2003 417 431 The authors used the novel technique of antibody-positioned RNA amplification in combination with microarrays to identify novel FMRP-target mRNAs. In the Fmr1 knockout mouse model for fragile X syndrome, many of these targets displayed altered expression and localization patterns within neurons and dendrites at both mRNA and protein levels.
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The fragile X syndrome protein FMRP associates with BC1 RNA and regulates the translation of specific mRNAs at synapses
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F. Zalfa, M. Giorgi, B. Primerano, A. Moro, A. Di Penta, S. Reis, B. Oostra, and C. Bagni The fragile X syndrome protein FMRP associates with BC1 RNA and regulates the translation of specific mRNAs at synapses Cell 112 2003 317 327 The authors observed increased mRNA levels within polysomes for several mRNAs isolated from brain and synaptic fraction of Fmr1 knockout mice. FMRP appears to interact with these mRNAs through BC1 RNA.
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Dendritic BC1 RNA: Functional role in regulation of translation initiation
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H. Wang, A. Iacoangeli, S. Popp, I.A. Muslimov, H. Imataka, N. Sonenberg, I.B. Lomakin, and H. Tiedge Dendritic BC1 RNA: functional role in regulation of translation initiation J Neurosci 22 2002 10232 10241
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0031310667
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FMRP associates with polyribosomes as an mRNP and the I304N mutation of severe fragile X syndrome abolishes this association
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Y. Feng, D. Absher, D.E. Eberhart, V. Brown, H.E. Malter, and S.T. Warren FMRP associates with polyribosomes as an mRNP and the I304N mutation of severe fragile X syndrome abolishes this association Mol Cell 1 1997 109 118
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P. Jin, D.C. Zarnescu, S. Ceman, M. Nakamoto, J. Mowrey, T.A. Jongens, D.L. Nelson, K. Moses, and S.T. Warren Biochemical and genetic interaction between the fragile X mental retardation protein and the microRNA pathway Nat Neurosci 7 2004 113 117 The precise molecular mechanism whereby FMRP affects translation of mRNAs at synapses has been largely unclear. Here, the authors have demonstrated an in vivo association between mammalian FMRP and microRNAs. FMRP was also shown to interact with the proteins Dicer and Argonaute 1, which are involved in the upstream processing of miRNA and siRNA precursors and formation of the RISC. The FMRP- micro ribonucleoprotein (miRNP) complex probably regulates translation at the level of elongation.
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Nat Neurosci
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Jin, P.1
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31
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0347382502
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Phosphorylation influences the translation state of FMRP-associated polyribosomes
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S. Ceman, W.T. O'Donnell, M. Reed, S. Patton, J. Pohl, and S.T. Warren Phosphorylation influences the translation state of FMRP-associated polyribosomes Hum Mol Genet 12 2003 3295 3305 The findings reported in this study implicate a role for FMRP phosphorylation in the translation of FMRP-associated mRNAs. Polysome profiles indicated that phosphorylated FMRP was resistant to 'run off' and associated with stalled polyribosomes, whereas unphosphorylated FMRP had fast 'run off' and was associated with actively translating polyribosomes. If these mechanisms also exist in the brain, synaptic stimulation might lead to the dephosphorylation of FMRP and its subsequent dissociation from the miRNP complex, which results in enhanced translation of mRNA.
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H.L. Zhang, T. Eom, Y. Oleynikov, S.M. Shenoy, D.A. Lieblet, J.B. Dictenberg, R.H. Singer, and G.J. Bassell Neurotrophin induced transport of β-actin mRNP complex increases β-actin levels and stimulates growth cone motility Neuron 31 2001 261 275
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T. Eom, L.N. Antar, R.H. Singer, and G.J. Bassell Localization of an β-actin mRNP complex with zipcode binding protein modulates the density of dendritic filopodia and filopodial synapses J Neurosci 23 2003 10433 10444
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