Observing in-phase single-quantum 15N multiplets for NH2 / NH3+ groups with two-dimensional heteronuclear correlation spectroscopy

Journal of Magnetic Resonance

Volumn 194, Issue 2, 2008, Pages 313-316

  Takayama, Yuki ^a   Sahu, Debashish ^a   Iwahara, Junji ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

15N; AX2 spin system; AX3 spin system; Heteronuclear correlation; Multiplets

Indexed keywords

15N; AX2 SPIN SYSTEM; HETERONUCLEAR CORRELATION; IN-PHASE; MULTIPLETS;

TWO DIMENSIONAL;

AMMONIA; NITROGEN;

ARTICLE; CHEMISTRY; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; QUANTUM THEORY; SPIN LABELING; STATISTICS;

AMMONIA; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; QUANTUM THEORY; SPIN LABELS; STATISTICS AS TOPIC;

EID: 52949107554     PISSN: 10907807     EISSN: 10960856     Source Type: Journal    
DOI: 10.1016/j.jmr.2008.07.018     Document Type: Article

References (21)

1. Kumar A., Rani Grace R.C., and Madhu P.K. Cross-correlations in NMR. Prog. Nucl. Magn. Reson. Spec. 37 (2000) 191-319
2. 15N transverse relaxation. J. Am. Chem. Soc. 129 (2007) 2971-2980
3. 15N heteronuclear correlation spectroscopy. J. Am. Chem. Soc. 128 (2006) 15388-15389
4. 13C NMR spectroscopy of methyl groups in very high molecular weight proteins and protein complexes. J. Am. Chem. Soc. 125 (2003) 10420-10428
5. 3 spin systems via polarization-transfer techniques. J. Magn. Reson. 100 (1992) 538-558
6. Cano K.E., Thrippleton M.J., Keeler J., and Shaka A.J. Cascaded z-filters for efficient single-scan suppression of zero-quantum coherence. J. Magn. Reson. 167 (2004) 291-297
7. 0 fields. J. Magn. Reson. A 105 (1993) 167-183
8. Thrippleton M.J., and Keeler J. Elimination of zero-quantum interference in two-dimensional NMR spectra. Angew. Chem. Int. Ed. Engl. 42 (2003) 3938-3941
9. Ollerenshaw J.E., Tugarinov V., and Kay L.E. Methyl TROSY: explanation and experimental verification. Magn. Reson. Chem. 41 (2003) 843-852
10. 15N NMR spectroscopy. Biochemistry 34 (1995) 16608-16617
11. Miclet E., Williams Jr. D.C., Clore G.M., Bryce D.L., Boisbouvier J., and Bax A. Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic acids. J. Am. Chem. Soc. 126 (2004) 10560-10570
12. Takayama Y., Castaneda C.A., Chimenti M., Garcia-Moreno B., and Iwahara J. Direct evidence for deprotonation of a lysine side chain buried in the hydrophobic core of a protein. J. Am. Chem. Soc. 130 (2008) 6714-6715
13. 2O in protein NMR. Application to sensitivity enhancement and NOE measurements. J. Am. Chem. Soc. 115 (1993) 12593-12594
14. Kupče E., Boyd J., and Campbell I.D. Short selective pulses for biochemical applications. J. Magn. Reson. B 106 (1995) 300-303
15. Iwahara J., and Clore G.M. Detecting transient intermediates in macromolecular binding by paramagnetic NMR. Nature 440 (2006) 1227-1230
16. Iwahara J., and Clore G.M. Direct observation of enhanced translocation of a homeodomain between DNA cognate sites by NMR exchange spectroscopy. J. Am. Chem. Soc. 128 (2006) 404-405
17. Iwahara J., Zweckstetter M., and Clore G.M. NMR structural and kinetic characterization of a homeodomain diffusing and hopping on nonspecific DNA. Proc. Natl. Acad. Sci. USA 103 (2006) 15062-15067
18. Sahu D., Clore G.M., and Iwahara J. TROSY-based z-exchange spectroscopy: application to the determination of the activation energy for intermolecular protein translocation between specific sites on different DNA molecules. J. Am. Chem. Soc. 129 (2007) 13232-13237
19. Delaglio F., Grzesiek S., Vuister G.W., Zhu G., Pfeifer J., and Bax A. NMRPipe - a multidimensional spectral processing system based on Unix pipes. J. Biomol. NMR 6 (1995) 277-293
20. Johnson B.A., and Blevins R.A. NMRView - a computer-program for the visualization and analysis of NMR data. J. Biomol. NMR 4 (1994) 603-614
21. 1H paramagnetic relaxation enhancement. J. Am. Chem. Soc. 126 (2004) 12800-12808