Direct observation of enhanced translocation of a homeodomain between DNA cognate sites by NMR exchange spectroscopy

Journal of the American Chemical Society

Volumn 128, Issue 2, 2006, Pages 404-405

  Iwahara, Junji ^a   Clore, G Marius ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HOMEODOMAIN PROTEIN;

ARTICLE; BASE PAIRING; COMPLEX FORMATION; DNA BINDING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN DNA INTERACTION; PROTEIN DOMAIN; SOLUBILIZATION; TECHNIQUE;

BASE SEQUENCE; DNA; GENES, HOMEOBOX; HOMEODOMAIN PROTEINS; KINETICS; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; SUBSTRATE SPECIFICITY;

EID: 31544448614     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja056786o     Document Type: Article

References (14)

1. Ernst, R. R.; Bodenhausen, G.; Wokaun, A. Principles of Nuclear Magnetic Resonance in One and Two Dimensions; Clarendon Press: Oxford, 1987.
2. (a) Catron, K. M.; Iler, N.; Abate, C. Mol. Cell Biol. 1993, 13, 2354-2365.
3. (b) Affolter, M.; Percival-Smith, A.; Müller, M.; Leupin, W.; Gehring, W. J. Proc. Natl. Acad. Sci. U.S.A. 1990, 87, 4093-4097.
4. (c) Frankel, E.; Pabo, C. O. Nat. Struct. Biol. 1998, 5, 692-696.
5. (a) Misteli, T. Science 2001, 291, 843-847.
6. (b) Hager, G. L.; Elbi, C.; Becker, M. Curr. Opin. Genet. Devel. 2002, 12, 137-141.
7. (a) Farrow, N. A.; Zhang, O.; Forman-Kay, J. D.; Kay, L. E. J. Biomol. NMR 1994, 4, 727-734.
8. (b) Montelione, G. T.; Wagner, G. J. Am. Chem. Soc. 1989, 111, 3096-3098.
9. In this instance the affinity of the protein for the two oligonucleotides is comparable. However, the experiment can still be applied to systems where the affinities for the two oligonucleotides are very different by appropriately adjusting the ratios of the three components (see Supporting Information).
10. McConnell, H. M. J. Chem. Phys. 1958, 28, 430-431.
11. (a) Berg, O. G.; Winter, R. B.; von Hippel, P. H. Biochemistry 1981, 20, 6929-6948.
12. (b) Halford, S. E.; Marko, J. F. Nucleic Acids Res. 2004, 32, 3040-3052.
13. Lewin, B. Genes VII; Oxford University Press: Oxford; 2000; p 545.
14. Chance, E. M.; Curtis, A. R., Jones, I. P.; Kirby, C. R. FACSIMILE: a computer program for flow and chemistry simulation and general initial value problems; Atomic Energy Research Establishment Report R8775; Harwell, H.M. Stationery Office: London, U.K., 1979.