Isolation and identification of a strain producing cold-adapted β-galactosidase, and purification and characterisation of the enzyme

Czech Journal of Food Sciences

Volumn 26, Issue 4, 2008, Pages 284-290

  Liu, Wen Yu ^a,b   Shi, Ying Wu ^a   Wang, Xin Qin ^a,c   Wang, Yun ^a   Wei, Chang Qing ^b   Lou, Kai ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b SHIHEZI UNIVERSITY   (China)

^c XINJIANG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Cold adapted galactosidase; Lactose hydrolysis; Psychrotrophic microorganism

Indexed keywords

RAHNELLA AQUATILIS;

EID: 52649161249     PISSN: 12121800     EISSN: None     Source Type: Journal    
DOI: 10.17221/31/2008-cjfs     Document Type: Article

References (20)

1. AKOLKAR S.K., SAJGURE A., LELE S.S. (2005): Lactase production from Lactobacillus acidophilus. World Journal of Microbiology and Biotechnology, 21:1119-1112.
2. BRADFORD M.M. (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
3. COKER J., PETER P., LOVELAND J., BRENCHLEY J.E. (2003): Biochemical characterization of a β-galactosidase with a low temperature optimum obtained from an Antarctic Arthrobacter isolate. Journal of Bacteriology, 185: 5473-5482.
4. FERNANDES S., GEUEKE B., DELGADO O. (2002): β-galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis. Applied Microbiology and Biotechnology, 58: 313-321.
5. th Ed. Willians & Wikiins, Baltimore.
6. HOYOUX A., JENNES I., GERDAY C. (2001): Cold-adapted β-galactosidase from the Antarctic psychrophile Pseudoalteromonas haloplanktis. Applied and Environmental Microbiology, 67:1529-1535.
7. HUBERT C, ANETA B, MAKOWSKI K., TURKIEWICZ M. (2005): Cloning, espression, and purification of recombinant cold-adapted β-galactosidase from anatarctic bacterium Pseudoalteromonas sp.22b. Protein Expression and Purification, 39: 27-34.
8. KUMAR S., TAMURA K., NEI M. (2004): MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics, 5:150-163.
9. LOVELAND J., GUTSHALL K., KASMIR J., PREMA P., BRENCHLEY J.E. (1993): Characterization of psychrotrophic microorganisms producing β-galactosidase activities. Applied Environment Microbiology, 60:12-18.
10. LOVELAND J., GUTSHALL K., KASMIR J., PREMA P., BRENCHLEY J.E. (1994): Characterization of a psychrotrophic Arthrobacter gene and its cold-active β- galactosidase. Applied Environment Microbiology, 60: 4544-4552.
11. MORITA T.Y.H. (1975 ): Psychrophilic bacteria. Bacteriology, 39: 144-167.
12. NAKAGAWA T., FUJIMOTO Y., UCHINO M. (2003): Isolation and characterization of psychrophiles producing cold-active β-galactosidase. Letters in Applied Microbiology, 37: 154-157.
13. NAKAGAWA T., IKEHATA R., UCHINO M. (2006a): Cold-active β-galactosidase activity of isolated psychrophilic- basidiomycetous yeast Guehomyces pullulans. Microbiological Research, 161: 75-79.
14. NAKAGAWA T., FUJIMOTO Y., IKEHATA R. (2006b): Purification and molecular characterization of cold-active β-galactosidase from Arthrobacter psychrolactophilus strain F2. Applied Microbiology and Biotechnology, 72: 720-725.
15. NAKAGAWA T., IKEHATA R., TOMIZUKA N. (2007): Overexpression and functional analysis of cold-active β-galactosidase from Arthrobacter psychrolactophilus strain F2. Protein Expression and Purification, 54: 295-299.
16. PARK J.-W, OH Y.-S., LIM J.-Y, ROH D.-H. (2006): Isolation and characterization of cold-adapted strains producing β-galactosidase. Journal of Microbiology, 44: 396-402.
17. SAITOU N., NEI M. (1987): The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4: 406-425.
18. TRIMBUR D., GUTSHALL K., KEVIN R., PREMA P., BRENCHLEY J.E. (1994): Characterization of a psychrotrophic Arthrobacter gene and its cold-active β- galactosidase. Applied and Environmental Microbiology, 60: 4544-4552.
19. THOMPSON J.D., GIBSON T.J., PLEWNIAK F., JEANMOUGIN F., HIGGINS D.G. (1997): The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25: 4876-4882.
20. TURKIEWICZ M., KALINOWSKA H., STANISLAW B. (2003): Antarctic marine bacterium Pseudoalteromons sp. 22b as a source of cold-adapted β-galactosidase. Biomolecular Engineering, 20: 317-324.