Pepsin extraction from bovine stomach using aqueous two-phase systems: Molecular mechanism and influence of homogenate mass and phase volume ratio

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 873, Issue 2, 2008, Pages 133-138

  Imelio, Natalia ^a   Marini, Analía ^a   Spelzini, Darío ^a   Picó, Guillermo ^a   Farruggia, Beatriz ^a  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

Author keywords

Aqueous two phase system; Bovine stomach; Partition; Pepsin

Indexed keywords

AMMONIUM SULPHATE; AQUEOUS TWO-PHASE SYSTEM; AQUEOUS TWO-PHASE SYSTEMS; BOVINE STOMACH; GASTRIC ACIDS; HYDROPHOBIC CHARACTER; MOLECULAR MECHANISMS; PARTITION; PARTITIONING BEHAVIOR; PEPSIN; PHASE VOLUME RATIO; POLYETHYLENE GLYCOLS; POLYMER-RICH PHASE; PREFERENTIAL INTERACTION; PROTEIN SURFACES; PROTEIN-POLYMER INTERACTION; PURIFICATION FACTORS; SODIUM CITRATES; TOTAL MASS; VOLUME RATIOS; WORKING CONDITIONS;

AMMONIUM COMPOUNDS; FLOW INTERACTIONS; HYDROCHLORIC ACID; POLYMERS; SODIUM; SOLVENT EXTRACTION; TWO PHASE FLOW;

BIOLOGICAL MATERIALS;

AMMONIUM SULFATE; CITRATE POTASSIUM; CITRATE SODIUM; MACROGOL; PEPSIN A; POLYMER; POTASSIUM DIHYDROGEN PHOSPHATE; SOLVENT;

ANALYTIC METHOD; AQUEOUS TWO PHASE SYSTEM; ARTICLE; COW; ENTROPY; EXTRACTION; HOMOGENATE; MATHEMATICAL COMPUTING; MOLECULAR INTERACTION; MOLECULAR MECHANICS; MOLECULAR WEIGHT; PHYSICAL PHASE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN ISOLATION; PROTEIN PURIFICATION; STOMACH; WATER STRUCTURE;

ANIMALS; CATTLE; CHEMICAL FRACTIONATION; PEPSIN A; PHOSPHATES; POLYETHYLENE GLYCOLS; POTASSIUM COMPOUNDS; STOMACH;

BOVINAE;

EID: 52349104092     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2008.07.006     Document Type: Article

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