Dependence of chymosin and pepsin partition coefficient with phase volume and polymer pausidispersity in polyethyleneglycol-phosphate aqueous two-phase system

Colloids and Surfaces B: Biointerfaces

Volumn 51, Issue 1, 2006, Pages 80-85

  Spelzini, Darío ^a   Picó, Guillemo ^a   Farruggia, Beatriz ^a  

^a Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

Author keywords

Aqueous two phase system; Chymosin; Partition; Pepsin

Indexed keywords

DISPERSIONS; MOLECULAR WEIGHT; PHOSPHATES; POLYETHYLENE GLYCOLS; PROTEINS;

AQUEOUS TWO-PHASE SYSTEMS; CHYMOSIN; PARTITION; PEPSIN;

ENZYME KINETICS;

CHYMOSIN; MACROGOL; PEPSIN A; PHOSPHATE; POLYMER;

AQUEOUS SOLUTION; ARTICLE; BINDING AFFINITY; MATHEMATICAL ANALYSIS; MOLECULAR WEIGHT; PARTITION COEFFICIENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN ISOLATION; STATISTICAL SIGNIFICANCE;

CHEMICAL FRACTIONATION; CHYMOSIN; MOLECULAR WEIGHT; PEPSIN A; PHASE TRANSITION; PHOSPHATES; POLYETHYLENE GLYCOLS; POLYMERS; WATER;

EID: 33746199924     PISSN: 09277765     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.colsurfb.2006.03.023     Document Type: Article

References (15)

1. Chitpinityol S., James M., and Crabbe C. Food Chem. 61 (1998) 395
2. Narita Y., Oda S., Moriyama A., and Kageyama T. Arch. Biochem. Biophys. 404 (2002) 177
3. Albertsson P.A. Partition of Cell Particles and Macromolecules. second ed. (1971), Wiley, New York
4. Zaslavsky B.Y. In: Zaslavsky B.Y. (Ed). Physical Chemistry and Bioanalytical Applications (1994), Marcel Dekker, New York
5. Lei X., Diamond A.D., and Hsu J.T. J. Eng. Data 35 (1990) 420
6. Forciniti D., Hall C., and Kula M. Bioseparation 2 (1991) 115
7. R. Hatti-Kaul (Ed.), Methods in Biotechnology. Aqueous Two-Phase Systems. Humana Press.
8. Arakawa T., and Timasheff S. Biochemistry 24 (1985) 6756
9. Marcos J.C., Fonseca L.P., Ramalho M.T., and Cabral J.M.S. J. Chromatogr. B 711 (1998) 295
10. Huddleston J.G., Abelaida J., Wang R., and Lyddiatt A. J. Chromatogr. B 680 (1996) 31
11. Guan Y., Lilley T., and Treffry T. Macromolecules 26 (1993) 3971-3979
12. Kang C.-H., Lee C.-K., and Sandler S.I. Ind. Eng. Chem. Res. 28 (1989) 1537-1542
13. Reh G., Nerli B., and Picó G. J. Chromatogr. B 780 (2002) 389
14. Mayerhoff Z., Roberto C., and Franco T. Biochem. Eng. J. 18 (2004) 217
15. Picó G., Romanini D., Nerli B., and Farruggia. J. Chromatogr. B 830 (2006) 286