Advances in the ultrastructural study of the implant-bone interface by backscattered electron imaging

Micron

Volumn 39, Issue 8, 2008, Pages 1363-1370

  Wierzchos, J ^a   Falcioni, T ^b   Kiciak, A ^c   Wolinski J ^d   Koczorowski, R ^e   Chomicki, P ^f   Porembska, M ^f   Ascaso, C ^g  

^a UNIVERSITY OF LLEIDA   (Spain)

^b Ilerimplant S L   (Spain)

^c Orlowski Hospital Warsaw   (Poland)

^d KIELANOWSKI INSTITUTE OF ANIMAL PHYSIOLOGY AND NUTRITION   (Poland)

^e POZNAN UNIVERSITY OF MEDICAL SCIENCES   (Poland)

^f Department of Cranio Maxillo Facial Surgery   (Poland)

^g CSIC   (Spain)

Author keywords

Animal study; Bone cells; Bone formation; Implants; Interface; Osseointegration; SEM BSE

Indexed keywords

BACKSCATTERING; BIOCOMPATIBILITY; BLIND SOURCE SEPARATION; CELLS; CYTOLOGY; DENTAL PROSTHESES; ELECTRON BEAM LITHOGRAPHY; ELECTRON SCATTERING; HEAVY METALS; IMAGING TECHNIQUES; MAPS; METALS; PAINTING; SECONDARY EMISSION; SURGERY; TITANIUM;

ANIMAL STUDY; BACK-SCATTERED ELECTRON IMAGING; BONE CELL RESPONSE; BONE CELLS; BONE FORMATION; BONE TISSUES; BONE-IMPLANT INTERFACES; CHEMICAL FIXATION; ELEMENT DISTRIBUTION MAPS; ENERGY DISPERSIVE X-RAY SPECTROMETRY; EXTRA CELLULAR MATRICES; HOST RESPONSE; HOST TISSUES; IMPLANT SURFACES; IMPLANT-BONE INTERFACES; IMPLANTS; INTERFACE; OSSEOINTEGRATION; SEM-BSE; TIME-BLOCK; TITANIUM IMPLANTS; ULTRASTRUCTURAL STUDY; VISUALISATION;

BONE;

TITANIUM;

ANIMAL; ARTICLE; BONE; BONE REGENERATION; ELECTRON PROBE MICROANALYSIS; FEMALE; SCANNING ELECTRON MICROSCOPY; SWINE; TOOTH IMPLANTATION; ULTRASTRUCTURE;

ANIMALS; BONE AND BONES; DENTAL IMPLANTS; ELECTRON PROBE MICROANALYSIS; FEMALE; MICROSCOPY, ELECTRON, SCANNING; OSSEOINTEGRATION; SWINE; TITANIUM;

ANIMALIA; SUIDAE;

EID: 52249120619     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micron.2008.01.022     Document Type: Article

References (26)

1. Bloebaum R.D., Bachus K.N., and Boyce T.M. Backscattered electron imaging: the role in calcified tissue and implant analysis. J. Biomater. Appl. 5 (1990) 56-85
2. Boyde A., and Jones S.J. Scanning electron microscopy of bone: instrument, specimens and tissues. Microsc. Res. Tech. 33 (1996) 92-120
3. Deligianni D.D., Katsala N., Ladas S., Sotiropoulou D., Amedee J., and Missirlis Y.F. Effect of surface roughness of the titanium alloy Ti-6Al-4V on human bone marrow cell response and on protein adsorption. Biomaterials 22 (2001) 1241-1251
4. Egger E.L., Gottsauner-Wolf F., Palmer J., Aro H.T., and Chao E.Y.S. Effects of axial dynamization on bone healing. J. Trauma 34 (1993) 185-192
5. Franch J., García F., Camón J., and Manzanares M.C. Backscattered electron imaging of the calcified tissues present in bone healing. Vet. Comp. Orthop. Traumatol. 11 (1998) 105-111
6. Hall J., and Lausmaa J. Properties of a new porous oxide surface on titanium implants. Appl. Osseointegr. Res. 1 (2000) 5-8
7. Huang H.H., Ho C.T., Lee T.H., Lee T.L., Liao K.K., and Chen F.L. Effect of surface roughness of ground titanium on initial cell adhesion. Biomol. Eng. 21 (2004) 93-97
8. Jasty M., Bragdon C.R., Schtzer S., Rubash H., Haire T., and Harris W.H. Bone ingrowth into porous coated canine total hip replacements. Quantification by backscattered scanning electron microscopy and image analysis. Scan. Microsc. 3 (1989) 1051-1057
9. Joy D.C. An introduction to Monte Carlo simulation. Scan. Microsc. 5 (1991) 329-337
10. Kim W., Caja V.L., Larsson S.A.B., and Chao E.Y.S. Advanced histomorphometric technique on the analysis of early fracture repair under external fixation. Int. J. Orthop. Trauma Suppl. 3 (1992) 5-10
11. Kotula P.G., Keenan M.R., and Michael J.R. Automated analysis of SEM X-ray spectral images: a powerful new microanalysis tool. Microsc. Microanal. 9 (2003) 1-17
12. Lavos-Valereto I.C., Wolynec S., Deboni M.C., and Koing Jr. B. In vitro and in vivo biocompatibility testing of Ti-6AI-7Nb alloy with and without plasma-sprayed hydroxyapatite coating. J. Biomed. Mater. Res. 58 (2001) 727-733
13. Li C.Y., Gao S.Y., Terashita T., Shimokawa T., Kawahara H., Matsuda S., and Kobayashi N. In vitro assays for adhesion and migration of osteoblastic cells (Saos-2) on titanium surfaces. Cell Tissue Res. 324 (2006) 369-375
14. Lincks J., Boyan B.D., Blanchard C.R., Lohmann C.H., Liu Y., Cochran D.L., Dean D.D., and Schwartz Z. Response of MG63 osteoblast like cells to titanium and titanium alloy is dependent on surface roughness and composition. Biomaterials 19 (1998) 2219-2232
15. Manzanares Céspedes M.C., Franch Serracanta J., Carvalho Lobato P., Montesdeoca Zaeza J.M., García Molina J.A., Greco Machado Y., Belmonte Calderón A.M., Delafuente Baigorri M.P., and Serra Renom I. The role of calcified tissues in the different stages of implant osteointegration. Dent. Med. Probl. 41 (2004) 179-191
16. Meyer U., Joos U., Mythili J., Stamm T., Hohoff A., Fillies T., Stratmann U., and Wiesmann H.P. Ultrastructural characterization of the implant/bone interface of immediately loaded dental implants. Biomaterials 25 (2004) 1959-1967
17. Ottani V., Raspanti M., Martini D., Tretola G., Ruggeri Jr., Franchi M., Giuliani Piccari G., and Ruggeri A. Electromagnetic stimulation of bone growth using backscattered electron imaging. Micron 33 (2002) 121-125
18. Reynolds S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17 (1963) 200-211
19. Roschger P., Fratzl P., Klaushofer K., and Rodan G. Mineralization of cancellous bone after alendronate and sodium fluoride treatment: a quantitative backscattered electron imaging study on minipig ribs. Bone 20 (1997) 393-397
20. Savarino L., Fini M., Ciapetti G., Cenni E., Granchi D., Baldini N., Greco M., Rizzi G., Giardino R., and Giunti A. Biologic effects of surface roughness and fluorhydroxyapatite coating on osteointegration in external fixation systems: an in vivo experimental study. J. Biomed. Mater. Res. Part A 66 (2003) 652-661
21. Simmons C.A., Valiquette N., and Pilliar R.M. Osseointegration of sintered porous-surfaced and plasma-spray coated implants: an animal model study of early post-implantation healing response and mechanical stability. J. Biomed. Mater. Res. 47 (1999) 127-138
22. Skedros J.G., Bloebaum R.D., Bachus K.N., and Boyce T.M. The meaning of greylevels in backscattered electron images of bone. J. Biomed. Mater. Res. 27 (1993) 47-56
23. Skedros J.G., Bloebaum R.D., Bachus K.N., Boyce T.M., and Constantz B. Influence of mineral content and composition on graylevels in backscattered electron images of bone. J. Biomed. Mater. Res. 27 (1993) 57-64
24. Skedros J.G., Sorenson S.M., and Jenson N.H. Are distributions of secondary osteon variants useful for interpreting load history in mammalian bones?. Cell Tissues Organs 185 (2007) 285-3007
25. Sul Y.T., Johansson C., Byon E., and Alberktsson T. The bone response of oxidized bioactive and non-bioactive titanium implants. Biomaterials 26 (2005) 6720-6730
26. Wierzchos J., and Ascaso C. Application of back-scattered electron imaging to the study of the lichen-rock interface. J. Microsc. 175 (1994) 54-59