Contribution of the collagen adhesin Acm to pathogenesis of Enterococcus faecium in experimental endocarditis

Infection and Immunity

Volumn 76, Issue 9, 2008, Pages 4120-4128

  Nallapareddy, Sreedhar R ^a,b   Singh, Kavindra V ^a,b   Murray, Barbara E ^a,b  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESIN; COLLAGEN; MESSENGER RNA;

ACM GENE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL CELL WALL; BACTERIAL ENDOCARDITIS; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM ISOLATE; DOWN REGULATION; ENTEROCOCCUS FAECIUM; FLOW CYTOMETRY; GENE LOCUS; GENE SEQUENCE; INSTITUTE FOR CANCER RESEARCH MOUSE; MOUSE; MULTIDRUG RESISTANCE; NONHUMAN; OPPORTUNISTIC INFECTION; PATHOGENESIS; PERITONITIS; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WILD TYPE;

ADHESINS, BACTERIAL; ANIMALS; ANTIBODIES, BACTERIAL; BACTERIAL ADHESION; COLLAGEN; ENDOCARDITIS, BACTERIAL; ENTEROCOCCUS FAECIUM; GENE DELETION; GENE EXPRESSION PROFILING; GRAM-POSITIVE BACTERIAL INFECTIONS; HUMANS; MICE; MUTAGENESIS, INSERTIONAL; SURVIVAL ANALYSIS; VIRULENCE FACTORS;

EID: 51949091016     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.00376-08     Document Type: Article

References (38)

1. Angrist, A. A., and M. Oka. 1963. Pathogenesis of bacterial endocarditis. JAMA 183:249-252.
2. Arduino, R. C., K. Jacques-Palaz, B. E. Murray, and R. M. Rakita. 1994. Resistance of Enterococcus faecium to neutrophil-mediated phagocytosis. Infect. Immun. 62:5587-5594.
3. Bayer, A. S., A. F. Bolger, K. A. Taubert, W. Wilson, J. Steckelberg, A. W. Karchmer, M. Levison, H. F. Chambers, A. S. Dajani, M. H. Gewitz, J. W. Newburger, M. A. Gerber, S. T. Shulman, T. J. Pallasch, T. W. Gage, and P. Ferrieri. 1998. Diagnosis and management of infective endocarditis and its complications. Circulation 98:2936-2948.
4. Elasri, M. O., J. R. Thomas, R. A. Skinner, J. S. Blevins, K. E. Beenken, C. L. Nelson, and M. S. Smeltzer. 2002. Staphylococcus aureus collagen adhesin contributes to the pathogenesis of osteomyelitis. Bone 30:275-280.
5. Giannitsioti, E., I. Skiadas. A. Antoniadou, S. Tsiodras, K. Kanavos, H. Triantafyllidi, and H. Giamarellon. 2007. Nosocomial vs. community-acquired infective endocarditis in Greece: changing epidemiological profile and mortality risk. Clin. Microbiol. Infect. 13:763-769.
6. Gillaspy, A. F., J. M. Patti, and M. S. Smeltzer. 1997. Transcriptional regulation of the Staphylococcus aureus collagen adhesion gene, cna. Infect. Immun. 65:1536-1540.
7. Gilot, P., P. Andre, and J. Content. 1999. Listeria monocytogenes possesses adhesins for fibronectin. Infect. Immun. 67:6698-6701.
8. Hienz, S. A., T. Schennings, A. Heimdahl, and J. I. Flock. 1996. Collagen binding of Staphylococcus aureus is a virulence factor in experimental endocarditis. J. Infect. Dis. 174:83-88.
9. Leavis, H. L., M. J. Bonten, and R. J. Willems. 2006. Identification of high-risk enterococcal clonal complexes: global dispersion and antibiotic resistance. Curr. Opin. Microbiol. 9:454-460.
10. Livak, K. J., and T. D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25:402-408.
11. McDonald, J. R., L. Olaison, D. J. Anderson, B. Hoen, J. M. Miro, S. Eykyn, E. Abrutyn, V. G. Fowler, Jr., G. Habib, C. Selton-Suty, P. A. Pappas, C. H. Cabell, G. R. Corey, F. Marco, and D. J. Sexton. 2005. Enterococcal endocarditis: 107 cases from the international collaboration on endocarditis merged database. Am. J. Med. 118:759-766.
12. Murray, B. E. 2000. Vancomycin-resistant enterococcal infections. N. Engl. J. Med. 342:710-721.
13. Murray, B. E., K. V. Singh, J. D. Heath, B. R. Sharma, and G. M. Weinstoek. 1990. Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites. J. Clin. Microbiol. 28:2059-2063.
14. Mylonakis, E., and S. B. Calderwood. 2001. Infective endocarditis in adults. N. Engl. J. Med. 345:1318-1330.
15. Nallapareddy, S. R., and B. E. Murray. 2006. Ligand-signaled upregulation of Enterococcus faecalis ace transcription, a mechanism for modulating host-E. faecalisw interaction. Infect. Immun. 74:4982-4989.
16. Nallapareddy, S. R., X. Qin, G. M. Weinstock, M. Hook, and B. E. Murray. 2000. Enterococcus faecalis adhesin, Ace, mediates attachment to extracellular matrix proteins collagen type IV and laminin as well as collagen type I. Infect. Immun. 68:5218-5224.
17. Nallapareddy, S. R., J. Sillanpaa, V. K. Ganesh, M. Hook, and B. E. Murray. 2007. Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm. Infect. Immun. 75:3192-3196.
18. Nallapareddy, S. R., K. V. Singh, R. W. Duh, G. M. Weinstoek, and B. E. Murray. 2000. Diversity of ace, a gene encoding a microbial surface component recognizing adhesive matrix molecules, from different strains of Enterococcus faecalis and evidence for production of Ace during human infections. Infect. Immun. 68:5210-5217.
19. Nallapareddy, S. R., K. V. Singh, and B. E. Murray. 2006. Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains. Appl. Environ. Microbiol. 72:334-345.
20. Nallapareddy, S. R., K. V. Singh, P. C. Okhuysen, and B. E. Murray. 2008. A. functional collagen adhesin gene, acm, in clinical isolates of Enterococcus faecium correlates with the recent success of this emerging nosocomial pathogen. Infect. Immun. 76:4110-4119.
21. Nallapareddy, S. R., K. V. Singh, J. Sillanpaa, D. A. Garsin, M. Hook, S. L. Erlandsen, and B. E. Murray. 2006. Endocarditis and biofilm-associated pili of Enterococcus faecalis. J. Clin. Investig. 116:2799-2807.
22. Nallapareddy, S. R., G. M. Weinstoek, and B. E. Murray. 2003. Clinical isolates of Enterococcus faecium exhibit strain-specific collagen binding mediated by Acm, a new member of the MSCRAMM family. Mol. Microbiol. 47:1733-1747.
23. Nannini, E. C., F. Teng, K. V. Singh, and B. E. Murray. 2005. Decreased virulence of a gls24 mutant of Enterococcus faecalis OGIRF in an experimental endocarditis model. Infect. Immun. 73:7772-7774.
24. Patti, J. M., B. L. Allen, M. J. McGavin, and M. Hook. 1994. MSCRAMM-mediated adherence of microorganisms to host tissues. Annu. Rev. Microbiol. 48:585-617.
25. Patti, J. M., T. Bremell, D. Krajewska-Pietrasik, A. Abdelnour, A. Tarkowski, C. Ryden, and M. Hook. 1994. The Staphylococcus aureus collagen adhesin is a virulence determinant in experimental septic arthritis. Infect. Immun. 62:152-161.
26. Rakita, R. M., V. C. Quan, K. Jacques-Palaz, K. V. Singh, R. C. Arduino, M. Mee, and B. E. Murray. 2000. Specific antibody promotes opsonization and PMN-mediated killing of phagocytosis-resistant Enterococcus faecium. FEMS Immunol. Med. Microbiol. 28:291-299.
27. Rhem, M. N., E. M. Lech, J. M. Patti, D. McDevitt, M. Hook, D. B. Jones, and K. R. Wilhelmus. 2000. The collagen-binding adhesin is a virulence factor in Staphylococcus aureus keratitis. Infect. Immun. 68:3776-3779.
28. Ryding, U., J. I. Flock, M. Flock, B. Soderquist, and B. Christensson. 1997. Expression of collagen-binding protein and types 5 and 8 capsular polysaccharide in clinical isolates of Staphylococcus aureus. J. Infect. Dis. 176:1096-1099.
29. Sambrook, J., E. F. Fritsch, and J. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. Singh, K. V., S. R. Nallapareddy, and B. E. Murray. 2007. Importance of the endocarditis and biofilm-associated pilus (ebp) locus in the pathogenesis of Enterococcus faecalis ascending urinary tract infection. J. Infect. Dis. 195: 1671-1677.
31. Singh, K. V., X. Qin, G. M. Weinstock, and B. E. Murray. 1998. Generation and testing of mutants of Enterococcus faecalis in a mouse peritonitis model. J. Infect. Dis. 178:1416-1420.
32. Smeltzer, M. S., A. F. Gillaspy, F. L. Pratt, and M. D. Thames. 1997. Comparative evaluation of use of cna, fnbA, fnbB, and hlb for genomic fingerprinting in the epidemiological typing of Staphylococcus aureus. J. Clin. Microbiol. 35:2444-2449.
33. Switalski, L. M., J. M. Patti, W. Butcher, A. G. Gristina, P. Speziale, and M. Hook. 1993. A. collagen receptor on Staphylococcus aureus strains isolated from patients with septic arthritis mediates adhesion to cartilage. Mol. Microbiol. 7:99-107.
34. Vernachio, J., A. S. Bayer, T. Le, Y. L. Chai, B. Prater, A. Schneider, B. Ames, P. Syribeys, J. Robbins, and J. M. Patti. 2003. Anti-clumping factor A. immunoglobulin reduces the duration of methicillin-resistant Staphylococcus aureus bacteremia in an experimental model of infective endocarditis. An-timicrob. Agents Chemother. 47:3400-3406.
35. Wilson, K. 1994. Preparation of genomic DNA from bacteria, p.2.4.1-2.4.2. In F. M. Ausubel, R. Brent, R. E. Kingston, D. M. David, J. G. Scidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology. Green Publishing Associates, Brooklyn, NY.
36. Xiong, Y. Q., W. Van Wamel, C. C. Nast, M. R. Yeaman, A. L. Cheung, and A. S. Bayer. 2002. Activation and transcriptional interaction between agr RNAII and RNAIII in Staphylococcus aureus in vitro and in an experimental endocarditis model. J. Infect. Dis. 186:668-677.
37. Xu, Y., L. Jiang, B. E. Murray, and G. M. Weinstock. 1997. Enterococcus faecalis antigens in human infections. Infect. Immun. 65:4207-4215.
38. Xu, Y., J. M. Rivas, E. L. Brown, X. Liang, and M. Hook. 2004. Virulence potential of the staphylococcal adhesin CNA in experimental arthritis is determined by its affinity for collagen. J. Infect. Dis. 189:2323- 2333.