Optimising the precision for localising fluorescent proteins in living cells by 2D Gaussian fitting of digital images: Application to COPII-coated endoplasmic reticulum exit sites

European Biophysics Journal

Volumn 37, Issue 8, 2008, Pages 1335-1349

  Spence, Peter ^a   Gupta, Vijay ^a   Stephens, David J ^a   Hudson, Andrew J ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Electron multiplied charge coupled devices; Fluorescence spectroscopy; Live cell microscopy; Molecular motors; Particle tracking; Theoretical models

Indexed keywords

COAT PROTEIN COMPLEX II; KINESIN; MOLECULAR MOTOR; YELLOW FLUORESCENT PROTEIN;

ALGORITHM; ARTICLE; CONTROLLED STUDY; DIGITAL IMAGING; ENDOPLASMIC RETICULUM; EPIFLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; MATHEMATICAL COMPUTING; MICROTUBULE; NORMAL DISTRIBUTION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SIGNAL NOISE RATIO;

ALGORITHMS; CELL SURVIVAL; COMPUTER SIMULATION; COP-COATED VESICLES; ENDOPLASMIC RETICULUM; FLUORESCENCE; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; NORMAL DISTRIBUTION; SENSITIVITY AND SPECIFICITY; SIGNAL PROCESSING, COMPUTER-ASSISTED;

MAMMALIA;

EID: 51849167647     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00249-008-0343-7     Document Type: Article

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