Two-dimensional gel electrophoresis of wool intermediate filament proteins

Journal of Proteomics

Volumn 71, Issue 4, 2008, Pages 439-447

  Paton, Louise N ^a,b   Gerrard, Juliet A ^a   Bryson, Warren G ^b,c  

^a UNIVERSITY OF CANTERBURY   (New Zealand)

^b AGRESEARCH   (New Zealand)

^c NONE   (New Zealand)

Author keywords

2D PAGE; Extraction method; Intermediate filament protein; Wool protein

Indexed keywords

INTERMEDIATE FILAMENT PROTEIN; KERATIN; POLYACRYLAMIDE GEL;

ARTICLE; DIALYSIS; EXTRACTION; FREEZE DRYING; PRIORITY JOURNAL; PROTEIN ANALYSIS; REHYDRATION; SEPARATION TECHNIQUE; TWO DIMENSIONAL GEL ELECTROPHORESIS; WOOL;

ANIMALS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; INTERMEDIATE FILAMENT PROTEINS; ISOELECTRIC POINT; OXIDATION-REDUCTION; SHEEP; WOOL;

EID: 51849135152     PISSN: 18743919     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jprot.2008.06.008     Document Type: Article

References (50)

1. Fuchs E., and Weber K. Intermediate filaments: structure, dynamics, function, and disease. Ann Rev Biochem 63 (1994) 345-382
2. Powell B.C., and Rogers G.E. The role of keratin proteins and their genes in the growth, structure and properties of hair. In: Höcker H. (Ed). Formation and Structure of Human Hair (1997), Birkhäuser Verlag, Basel/Switzerland 59-148
3. Crewther W.G., Dowling L.M., Gough K.H., Inglis A.S., McKern N.M., Sparrow L.G., and Woods E.F. The low-sulphur proteins of wool: studies on their classification, characterisation, primary and secondary structure. Proceedings of the 5th International Wool Textile Research Conference. Aachen, Germany (1975) 233-242
4. Powell B.C., and Beltrame J.S. Characterisation of a hair (wool) keratin intermediate filament gene domain. J Invest Dermatol 102 (1994) 171-177
5. Heid H.W., Werner E., and Franke W.W. The complement of native alpha-keratin polypeptides of hair-forming cells: a subset of eight polypeptides that differ from epithelial cytokeratins. Differentiation 32 (1986) 101-119
6. Fraser R.D.B., MacRae T.P., and Rogers G.E. Keratins (1972), Charles C Thomas, Springfield, Illinois, USA 1-304
7. Goddard D.R., and Michaelis L. A study on keratin. J Biol Chem 106 (1934) 605-614
8. Parry D.A.D., and Steinert P.M. Intermediate Filament Structure (1995), Springer-Verlag, Heidelberg 183
9. Achtstaetter T., Hatzfeld M., Quinlan R.A., Parmelee D.C., and Franke W.W. Separation of cytokeratin polypeptides by gel electrophoresis and chromatographic techniques and their identification by immunoblotting. Methods Enzymol 34 (1986) 355-371
10. Plowman J.E. Proteomic database of wool components. J Chromatogr B 787 (2003) 63-76
11. Woods J.L., and Orwin D.F.G. Wool proteins of New Zealand Romney sheep. Aust J Biol Sci 40 (1987) 1-14
12. Marshall R.C. Analysis of the proteins from single wool fibers by two-dimensional polyacrylamide gel electrophoresis. Tex Res J 51 (1981) 106-108
13. Herbert B.R., and Woods J.L. Immobilised pH gradient isoelectric focusing of wool proteins. Electrophoresis 15 (1994) 972-976
14. Herbert B.R., Molloy M.P., Yan J.X., Gooley A.A., Bryson W.G., and Williams K.L. Characterisation of wool intermediate filament proteins separated by micropreparative two-dimensional electrophoresis. Electrophoresis 18 (1997) 568-572
15. Plowman J.E., Bryson W.G., Flanagan L.M., and Jordan T.W. Problems associated with the identification of proteins in homologous families: the wool keratin family as a case study. Anal Biochem 300 (2002) 221-229
16. O'Donnell I.J., and Thompson E.O.P. Studies on reduced wool. IV. The isolation of a major component. Aust J Biol Sci 17 (1964) 973-989
17. Schweizer J., Bowden P.E., Coulombe P.A., Langbein L., Lane E.B., Magin T.M., Maltais L., Omary M.B., Parry D.A.D., Rogers M.A., and Wright M.W. New consensus nomenclature for mammalian keratins. J Cell Biol 17 (2006) 169-174
18. Crewther W.G., Dowling L.M., Gough K.H., Marshall R.C., and Sparrow L.G. The Microfibrillar proteins of alpha-keratin. In: Creamer L.K. (Ed). Fibrous Proteins: Scientific, Industrial and Medical Aspects (1980), Academic Press Inc. (London) Ltd., London 151-159
19. Dowling L.M., Crewther W.G., and Parry D.A.D. Secondary structure of component 8c-1 of alpha-keratin. Biochem J 236 (1986) 705-712
20. Sparrow L., Robinson C., Caine J., McMahon D., and Strike P. Type II intermediate-filament proteins from wool. The amino acid sequence of component 5 and comparison with component 7c. Biochem J 282 (1992) 291-297
21. Wilson B.W., Edwards K.J., Sleigh M.J., Byrne C.R., and Ward K.A. Complete sequence of a type-I microfibrillar wool keratin gene. Gene 73 (1988) 21-31
22. Marshall R.C., and Blagrove R.J. Successful isoelectric focusing of wool low-sulphur proteins. J Chromatogr 172 (1979) 351-356
23. Sabounchi-Schütt F., Åström J., Olsson I., Eklund A., Grunewald J., and Bjellqvist B. An immobiline drystrip application method enabling high-capacity two-dimensional gel electrophoresis. Electrophoresis 21 (2000) 3649-3656
24. Görg A., Obermaier C., Boguth G., Harder A., Scheibe B., Wildgruber R., and Weiss W. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 21 (2000) 1037-1053
25. Görg A., Boguth G., Obermaier C., Posch A., and Weiss W. Two-dimensional polyacrylamide gel electrophoresis with immobilized ph gradients in the first dimension (IPG-Dalt): the state of the art and the controversy of vertical versus horizontal systems. Electrophoresis 16 (1995) 1079-1086
26. Scheler C., Lamer S., Pan Z., Li X.P., Salnikow J., and Jungblut P. Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Electrophoresis 19 (1998) 918-927
27. Plowman J.E., Bryson W.G., and Jordan T.W. Application of proteomics for determining protein markers for wool quality traits. Electrophoresis 21 (2000) 1899-1906
28. Plowman J.E., Flanagan L.M., Paton L.N., Fitzgerald A.C., Joyce N.I., and Bryson W.G. The effect of oxidation or alkylation on the separation of wool keratin proteins by two-dimensional gel electrophoresis. Proteomics 3 (2003) 942-950
29. Herbert B.R., Molloy M.P., Gooley A.A., Walsh B.J., Bryson W.G., and Williams K.L. Improved protein solubility in two-dimensional electrophoresis using tributyl phosphine as reducing agent. Electrophoresis 19 (1998) 845-851
30. Herbert B.R. Advances in protein solubilisation for two-dimensional electrophoresis. Electrophoresis 20 (1999) 660-663
31. Berkelman T. Use of a novel reductant for improved 2-D separations of basic proteins. Proteomics Forum (2001) Munich
32. Berkelman T., and Steastedt T. In: AB (Ed). 2D electrophoresis using immobilized ph gradients. principles and methods (1998), Amersham Pharmacia Biotech, Uppsala, Amersham 1-100
33. Görg A., Obermaier C., Boguth G., and Weiss W. Recent developments in two-dimensional gel electrophoresis with immobilized pH gradients: wide pH gradients up to pH 12, longer separation distances and simplified procedures. Electrophoresis 20 (1999) 712-717
34. Sanchez J.C. Sample preparation and solubilization: crucial steps preceding the two-dimensional gel electrophoresis process. ABRF. San Diego (1998) 1-16
35. Molloy M.O., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.C., Hochstrasser D.F., Williams K.L., and Gooley A.A. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis 19 (1998) 837-844
36. Rabilloud T., Adessi C., Giraudel A., and Lunardi J. Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 18 (1997) 307-316
37. Rabilloud T. Use of thiourea to increase the solubility of membrane proteins in two-dimensional electrophoresis. Electrophoresis 19 (1998) 758-760
38. Yan J.X., Sanchez J.C., Rouge V., Williams K.L., and Hochstrasser D.F. Modified immobilized pH gradient gel strip equilibration procedure in SWISS-2DPAGE protocols. Electrophoresis 20 (1999) 723-726
39. Galvani M., Rovatti L., Hamdan M., Herbert B.R., and Righetti P.G. Protein alkylation in the presence/absence of thiourea in proteome analysis: a matrix assisted laser desorption/ionization-time of flight-mass spectrometry investigation. Electrophoresis 22 (2001) 2066-2074
40. Weistermeiner R., and Naven T. Proteomics in Practice (2002), Wiley-VCH Verlag-GmbH Weinheim, Freiburg 316
41. Görg A., Weiss W., and Dunn M.J. Current two-dimensional electrophoresis technology for proteomics. Proteomics 4 (2004) 3665-3685
42. O'Farrell P.H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250 (1975) 4007-4021
43. Harder A., Wildgruber R., Nawrocki A., Fey S.J., Larsen P.M., and Görg A. Comparison of yeast cell protein solubilization procedures for two-dimensional electrophoresis. Electrophoresis 20 (1999) 826-829
44. Galvani M., Hamdan M., Herbert B.R., and Righetti P.G. Alkylation kinetics of proteins in preparation for two-dimensional maps: a matrix assisted laser desorption/ionization-mass spectrometry investigation. Electrophoresis 22 (2001) 2058-2065
45. Harrap B.S., and Gillespie J.M. A further study on the extraction of reduced proteins from wool. Aust J Biol Sci 16 (1963) 542-557
46. Caldwell J.P., Mastronarde D.N., Woods J.L., and Bryson W.G. The three-dimensional arrangement of intermediate filaments in Romney wool cortical cells. J Struct Biol 151 (2005) 298-305
47. Caldwell J.P., Woods J.L., and Bryson W.G. Electron tomography of merino wool fibre ultrastructure. Proc. 11th Int. Wool Res. Conf., Leeds, UK (2005) CD publication reference 107FWSA
48. Plowman J.E., Paton L.N., and Bryson W.G. The differential expression of proteins in the cortical cells of wool and hair fibres. Review article. Exp Dermatol 16 (2007) 707-714
49. Plowman J.E., and Bryson W.G. Protein composition of orthocortical and paracortical cells in New Zealand merino wool fibres. Proceedings of the International Wool Textile Research Conference, Leeds, U.K. (2005) CD publication
50. Bryson W.G., Plowman J.E., Flanagan L.M., and Jordan W.T. The analytical tools of proteomics provide new insight into expression of the wool genome, keratin chemistry and textile processing. Wool Techno Sheep Breed 49 (2001) 246-260