Pitfalls in the measurement of tissue DDAH activity: Is DDAH sensitive to nitrosative and oxidative stress?

Kidney International

Volumn 74, Issue 7, 2008, Pages 969-

  Tsikas, Dimitrios ^a   Chobanyan, Kristine ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

2,3 DIMETHOXY 1,4 NAPHTHOQUINONE; 3,3 BIS(2 AMINOETHYL) 1 HYDROXY 2 OXOTRIAZENE; DIMETHYLARGININASE; N ETHYLMALEIMIDE; NITRATE; NITRIC OXIDE; NITRITE;

COLORIMETRY; DRUG EFFICACY; DRUG INHIBITION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITION; HUMAN; IN VITRO STUDY; IN VIVO STUDY; KIDNEY CORTEX; LETTER; MASS FRAGMENTOGRAPHY; NITROSATIVE STRESS; OXIDATIVE STRESS; PRIORITY JOURNAL; SENSITIVITY ANALYSIS;

EID: 51849135069     PISSN: 00852538     EISSN: 15231755     Source Type: Journal    
DOI: 10.1038/ki.2008.360     Document Type: Letter

References (4)

1. Tain YL, Baylis C. Determination of dimethylarginine dimethylaminohydrolase activity in the kidney. Kidney Int 2007; 72: 886-889.
2. Prescott LM, Jones ME. Modified methods for the determination of carbamyl aspartate. Anal Biochem 1969; 32: 408-419.
3. Chobanyan K, Thum T, Suchy MT et al. GC-MS assay for hepatic DDAH activity in diabetic and non-diabetic rats by measuring dimethylamine (DMA) formed from asymmetric dimethylarginine (ADMA): evaluation of the importance of S-nitrosothiols as inhibitors of DDAH activity in vitro and in vivo in humans. J Chromatogr B 2007; 858: 32-41.
4. G-dimethylarginine dimethylaminohydrolase, from rat kidney. J Biol Chem 1989; 264: 10205-10209.