Characterization of cold-active uracil-DNA glycosylase from Bacillus sp. HJ171 and its use for contamination control in PCR

Applied Microbiology and Biotechnology

Volumn 80, Issue 5, 2008, Pages 785-794

  Kim, Gun A ^a   Lee, Mi Sun ^a   Sun, Younguk ^a   Lee, Byung Doo ^a   Lee, Jong Il ^a   Lee, Jung Hyun ^b   Kwon, Suk Tae ^a  

^a Sungkyunkwan University   (South Korea)

^b Korea Research Institute of Ships and Ocean Engineering   (South Korea)

Author keywords

Bacillus sp. HJ171 (Bsp HJ171); Bsp HJ171 UDG; Carry over contamination; Psychrophile; Uracil DNA glycosylase (UDG)

Indexed keywords

AMINATION; AMINES; AMINO ACIDS; BACTERIOLOGY; CLONING; CONCENTRATION (PROCESS); CONTAMINATION; COSMIC RAYS; DNA; ENZYMES; ESCHERICHIA COLI; FOOD ADDITIVES; GENE ENCODING; GENES; HEALTH; NUCLEIC ACIDS; PURIFICATION; SODIUM CHLORIDE;

ACID RESIDUES; AMINO-ACID SEQUENCES; BACILLUS SP.; BACILLUS SP. HJ171 (BSP HJ171); BSP HJ171 UDG; CARRY-OVER CONTAMINATION; COLD-ACTIVE; CONTAMINATION CONTROL; DNA GLYCOSYLASE; NACL CONCENTRATION; ONE STEP; OPTIMAL TEMPERATURES; POLYMERASE CHAIN REACTION; PSYCHROPHILE; PURIFICATION SYSTEMS; PURIFIED ENZYMES; SEQUENCE SIMILARITY; URACIL-DNA GLYCOSYLASE (UDG);

ORGANIC ACIDS;

AMINO ACID; POTASSIUM CHLORIDE; SODIUM CHLORIDE; URACIL DNA GLYCOSIDASE;

AMINO ACID; BACTERIUM; CONCENTRATION (COMPOSITION); DNA; ENZYME ACTIVITY; GENE EXPRESSION; POLYMERASE CHAIN REACTION; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACTERIUM CONTAMINATION; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENE IDENTIFICATION; GENE SEQUENCE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TEMPERATURE;

AMINO ACID SEQUENCE; BACILLUS; BACTERIAL PROTEINS; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; URACIL-DNA GLYCOSIDASE;

BACILLUS SP.; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 51849115413     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1585-0     Document Type: Article

References (27)

1. R Chen H Wang LM Mansky 2002 Roles of uracil-DNA glycosylase and dUTPase in virus replication J Gen Virol 83 2339 2345
2. S Chong GE Montello A Zhang EJ Cantor W Liao M-Q Xu J Benner 1998 Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step Nucleic Acids Res 26 5109 5115
3. JH Chung EK Im HY Park JH Kwon S Lee J Oh KC Hwang JH Lee Y Jang 2003 A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily Nucleic Acids Res 8 2045 2055
4. R Cone J Duncan H Hamilton EC Friedberg 1977 Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilis Biochemistry 16 3194 3201
5. F Corpet 1988 Multiple sequence alignment with hierarchical clustering Nucleic Acids Res 16 10881 10890
6. HA Erlich 1989 Polymerase chain reaction technology J Clin Immunol 9 437 447
7. JL Hartley A Rashtchian 1993 Dealing with contamination: enzymatic control of carryover contamination in PCR PCR Methods Appl 3 10 14
8. S Jaeger R Schmuck H Sobek 2000 Molecular cloning, sequencing, and expression of the heat-labile uracil-DNA glycosylase from a marine psychrophilic bacterium, strain BMTU3346 Extremophiles 4 115 122
9. SB Kleiboeker 2005 Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR Virol J 2 29
10. UK Laemmli 1970 Cleavage of structural protein during the assembly of the head of bacteriophage T4 Nature 227 680 685
11. O Lanes PH Guddal DR Gjellesvik NP Willassen 2000 Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua) Comp Biochem Physiol Part B 127 399 410
12. O Lanes I Leiros AO Smalas NP Willassen 2002 Identification, cloning, and expression of uracil-DNA glycosylase from Atlantic cod (Gadus morhua): characterization and homology modeling of the cold-active catalytic domain Extremophiles 6 73 86
13. T Lindahl 1974 An N-glycosidase from Escherichia coli that releases free uracil from DNA containing deaminated cytosine residues Proc Natl Acad Sci U S A 71 3649 3653
14. X Liu J Liu 2004 Cloning, expression, and characterization of uracil-DNA glycosylase of Chlamydia pneumoniae in Escherichia coli Protein Expr Purif 35 46 53
15. MC Longo MS Berninger JL Hartlev 1990 Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions Gene 93 125 128
16. OH Lowry NJ Rosebrough AL Farr RJ Randall 1951 Protein measurement with folin phenol reagent J Biol Chem 193 265 275
17. M Ritzler I Perschil M Altwegg 1999 Influence of residual uracil-DNA glycosylase activity on the electrophoretic migration of dUTP-containing PCR products J Microbiol Methods 35 73 76
18. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
19. F Sanger AR Coulson GF Hong DF Hill GB Petersen 1982 Nucleotide sequence of bacteriophage lambda DNA J Mol Biol 162 729 773
20. AA Sartori S Fits-Gibbon H Yang JH Miller J Jiricny 2002 A novel uracil-DNA glycosylase with broad substrate specificity and an unusual active site EMBO J 21 3182 3191
21. R Savva K McAuley-Hecht T Brown LH Pearl 1995 The structural basis of specific base-excision repair by uracil-DNA glycosylase Nature 373 487 493
22. MS Seo YJ Kim JJ Choi MS Lee JH Kim JH Lee ST Kwon 2007 Cloning and expression of a DNA ligase from the hyperthermophilic archaeon Staphylothermus marinus and properties of the enzyme J Biotechnol 128 519 530
23. HJ Shin SK Lee JJ Choi SH Koh JH Lee ST Kwon 2005 Cloning, Expression, and characterization of a family B-type DNA polymerase from the hyperthermophilic crenarchaeon Pyrobaculum arsenaticum and its application to PCR J Microbiol Biotechnol 15 1359 1367
24. H Sobek M Schmidt B Frey K Kaluza 1996 Heat-labile uracil-DNA glycosylase: purification and characterization FEBS Lett 388 1 4
25. EW Taggart KC Carroll CL Byington GA Crist DR Hillyard 2002 Use of heat labile UNG in an RT-PCR assay for enterovirus detection J Virol Methods 105 57 65
26. CG Thornton JL Hartely A Rashtchian 1992 Utilizing uracil DNA glycosylase to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling Biotechniques 13 180 183
27. EM Udaykumar JS Epstein IK Hewlett 1993 A novel method employing UNG to avoid carry-over contamination in RNA-PCR Nucleic Acids Res 21 3917 3918