Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43

Toxicology in Vitro

Volumn 22, Issue 7, 2008, Pages 1719-1725

  Zhou, De Rong ^a,b   Zhou, Yong Cui ^a   Cui, Guang Hui ^b   Guo, Xin ^b   Qin, Jie ^b   Gui, Yao Ting ^b   Cai, Zhi Ming ^b  

^a SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

^b PEKING UNIVERSITY SHENZHEN HOSPITAL   (China)

Author keywords

connexin; Connexin43; Cx; dimethyl sulphoxide; DMEM; DMSO; Dulbecco modified eagle medium; FCB; fetal calf serum; gap junctional intercellular communication; Gap junctional intercellular communication; GJIC; Gossypol; scrape loading and dye transfer; Sertoli cells; SLDT

Indexed keywords

CHOLECYSTOKININ OCTAPEPTIDE; CONNEXIN 43; DYE; GOSSYPOL;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL COMMUNICATION; CELL CULTURE; CELL LINE; CELL VIABILITY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG EFFECT; GAP JUNCTION; IMMUNOFLUORESCENCE; MALE; MOUSE; NONHUMAN; PROTEIN DETERMINATION; PROTEIN EXPRESSION; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SERTOLI CELL; SPERMATOGENESIS; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL COMMUNICATION; CELL LINE; CELL SURVIVAL; CONNEXIN 43; DOSE-RESPONSE RELATIONSHIP, DRUG; FLUORESCENT ANTIBODY TECHNIQUE; GAP JUNCTIONS; GENE EXPRESSION REGULATION; GOSSYPOL; MALE; MICE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SERTOLI CELLS; TIME FACTORS;

EID: 51749088100     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2008.07.012     Document Type: Article

References (24)

1. Batias C., Siffroi J.-P., Fenichel P., Pointis G., and Segretain D. Cx43 gene expression and regulation in the rodent seminiferous epithelium. Journal of Histochemistry and Cytochemistry 48 6 (2000) 793-805
2. Bruzzone R., White T.W., and Paul D.L. Connections with connexins: the molecular basis of direct intercellular signaling. European Journal of Biochemistry 238 1 (1996) 1-27
3. Brehm R., Zeiler M., Ruttinger C., Herde K., Kibschull M., et al. A Sertoli cell-specific knockout of connexin43 prevents initiation of spermatogenesis. American Journal of Pathology 171 1 (2007) 19-31
4. Carlsen E., Giwercman A., Keiding N., and Skakkebaek N.E. Evidence for the decreasing quality of semen during the past 50 years. British Medical Journal 305 6854 (1992) 609-613
5. Fiorini C., Tilloy-Ellul A., Chevalier S., Charuel C., and Pointis G. Sertoli cell junctional proteins as early targets for different classes of reproductive toxicants. Reproductive Toxicology 18 3 (2004) 413-421
6. Cristancho J.M., Campos de Carvalho A.C., and Varanda W.A. Short term regulation of cell-cell communication in TM3 Leydig cells. A perforated patch study. Biochimica et Biophysica Acta 1496 2-3 (2000) 325-332
7. Defamie N., Berthaut I., Mograbi B., Chevallier D., Dadoune J.P., Fenichel P., Segretain D., and Pointis G. Impaired gap junction connexin43 in Sertoli cells of patients with secretory azoospermia: a marker of undifferentiated Sertoli cells. Laboratory Investigation 83 3 (2003) 449-456
8. el-Fouly M.H., Trosko J.E., and Chang C.C. A rapid and simple technique to study gap junctional intercellular communication. Experimental Cell Research 168 2 (1987) 422-430
9. Gina S. Mixing of connexins in gap junction membrane channels. Proceedings of the National Academy of Sciences USA 92 20 (1995) 9210-9214
10. Gui Y., Zhang J., Yuan L., and Lessey B.A. Regulation of Hoxa-10 and its expression in normal and abnormal endometrium. Molecular Human Reproduction 5 (1999) 866-873
11. Haefliger J.A., Bruzzone R., Jenkins N.A., Gilbert Di., Copland N.G., and Paul D.L. Four novel members of connexin family of gap junction proteins. Journal of Biological Chemistry 267 3 (1992) 2057-2064
12. Herve J.-C., Pluciennik F., Bastide B., Cronier L., Verrecchia F., Malassin A., Joffre M., and Deleze J. Contraceptive gossypol blocks cell-to-cell communication in human and rat cells. European Journal of Pharmacology 313 3 (1996) 243-255
13. McGinley D.M., Posalaky Z., Porvaznik M., and Russell L. Gap junctions between Sertoli and germ cells of rat seminiferous tubule. Tissue Cell 11 4 (1979) 741-754
14. Moh P.P., Chang G.C.J., Brueggemeier R.W., and Lin Y.C. Effect of gossypol on 5α-reductase and 5α-hydroxysteroid dehydrogenase activities in adult rat testes. Research Communications in Chemical Pathology Pharmacology 82 1 (1993) 12-26
15. Monsees T.K., Franz M., Gebhardt S., Winterstein U., Schill W.B., and Hayatpour J. Sertoli cells as a target for reproductive hazards. Andrologia 32 4-5 (2000) 239-246
16. Plum A., Hallas G., Magin T., Dombrowski F., Hagendorff A., Schumacher B., Wolpert C., Kim J.S., Lamers W.H., Evert M., Meda P., Straub O., and Willecke K. Unique and shared functions of different connexins in mice. Current biology 10 18 (2000) 1083-1091
17. Quian S.Z., and Wang Z.G. A potential antifertility agent for males. Annual Review of Pharmacology Toxicology 24 (1984) 329-360
18. Roscoe W.A., Barr K.J., Mhawi A.A., Pomerantz D.K., and Kidder G.M. Failure of spermatogenesis in mice lacking connexin43. Biology of Reproduction 65 3 (2001) 829-838
19. Sharpe R.M. Declining sperm counts in men-is there an endocrine cause?. Journal of Endocrinology 136 3 (1993) 357-360
20. Sridharan S., Simon L., Meling D.D., Cyr D.G., Gutstein D.E., Fishman G.I., Guillou F., and Cooke P.S. Proliferation of adult Sertoli cells following conditional knockout of the gap junctional protein GJA1 (connexin43) in mice. Biology of Reproduction 76 5 (2007) 804-812
21. Strøm-Hansen T., Cornett C., and Jaroszewski J.W. Interaction of gossypol with amino acids and peptides as a model of enzyme inhibition. International Journal of Peptide and Protein Research 34 4 (1989) 306-310
22. Tan I.P., Roy C., Saez J.C., Saez C.G., Paul D.L., and Risley M.S. Regulated assembly of connexin33 and connexin43 into rat Sertoli cell gap junctions. Biology of Reproduction 54 6 (1996) 1300-1310
23. Wu D.F., Yu Y.W., Tang Z.M., and Wang M.Z. Pharmacokinetics of (+/-)-, (+)-, and (-)- gossypol in humans and dogs. Clinical Pharmacology and Therapeutics 39 6 (1986) 613-618
24. Zhang J.-T., and Nicholson B. Sequence and tissue distribution of a second protein of hepatic gap junctions Cx26 as deduced from its cDNA. The Journal of Cell Biology 109(6 Pt 2) (1989) 3391-3401