Biological aspects of dichlorofluorescein measurement of cellular reactive oxygen species

Radiation Research

Volumn 170, Issue 3, 2008, Pages 408-

  Hafer, Kurt ^a   Schiestl, Robert H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DICHLOROFLUORESCEIN; REACTIVE OXYGEN METABOLITE;

ANIMAL CELL; ARTIFACT REDUCTION; CELL MEMBRANE PERMEABILITY; CELL TYPE; DEACETYLATION; DIAGNOSTIC PROCEDURE; DICHLOROFLUORESCEIN ASSAY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HYDROLYSIS; LETTER; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RADIATION EXPOSURE; SPECTROPHOTOMETRY;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; FLUORESCEINS; FREE RADICALS; OXIDATION-REDUCTION; REACTIVE OXYGEN SPECIES;

EID: 51649119600     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR1439c.1     Document Type: Letter

References (4)

1. P. Wardman, Use of the dichlorofluorescein assay to measure "reactive oxygen species". Radiat Res. 170, 406-407 (2008). [letter to the Editor]
2. D. A. Bass, J. W. Parce. L. R. Dechatelet, P. Szejda, M. C. Seeds and M. Thomas, Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol. 130, 1910-1917 (1983).
3. K. Hafer. T. Konishi and R. H. Schiestl, Radiation-induced long-lived extracellular radicals do not contribute to measurement of intracellular reactive oxygen species using the dichlorofluorescein method. Radiat. Res. 169, 469-473 (2008).
4. 2 in cultured endothelial cells. Arch. Biochem. Biophxs. 302, 348-355 (1993).