Use of the dichlorofluorescein assay to measure reactive oxygen species

Radiation Research

Volumn 170, Issue 3, 2008, Pages 406-407

  Wardman, Peter ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

3 NITROTYROSINE; 8 OXOGUANOSINE; DICHLORODIHYDROFLUORESCEIN; DICHLOROFLUORESCEIN; FLUORESCENT DYE; REACTIVE OXYGEN METABOLITE; SUPEROXIDE; UNCLASSIFIED DRUG;

DIAGNOSTIC PROCEDURE; DIHCLOROFLUORESCEIN ASSAY; FLUORESCENCE; HUMAN; HUMAN CELL; LETTER; OXIDATION; OXIDATIVE STRESS; PHOTOCHEMICAL EFFICIENCY; PHOTOOXIDATION; PRIORITY JOURNAL;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; FLUORESCEINS; FREE RADICALS; OXIDATION-REDUCTION; REACTIVE OXYGEN SPECIES;

EID: 51649121755     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR1439a.1     Document Type: Letter

References (10)

1. Y. N. Korystov, V. V. Shaposhnikova, A. F. Korystova and M. O. Emel'yanov, Detection of reactive oxygen species indiced by radiation in cells using the dichlorofluorescein assay. Radiat. Res. 168, 226-232 (2007).
2. K. Hafer, T. Konishi and R. H. Schiestl. Radiation-induced long-lived extracellular radicals do not contribute to measurement of intracellular reactive oxygen species using the dichlorofluorescein method. Radiat. Res. 169, 469-473 (2008).
3. X. S. Wan, P. Bloch, J. H. Ware, Z. Zhou, J. J. Donahue, J. Guan, J. Stewart and A. R. Kennedy, Detection of oxidative stress induced by low- and high-linear energy transfer radiation in cultured human epithelial cells. Radiat. Res. 163, 364-368 (2005).
4. P. Wardman, Fluorescent and luminescent probes for measurement of oxidative and nitrosative species in cells and tissues: progress, pitfalls, and prospects. Free Radic. Biol. Med. 43, 995-1022 (2007).
5. P. M. Easton, A. C. Simmonds, A. Rakishev, A. M. Egorov and L. P. Candeias, Quantitative model of the enhancement of peroxidase-in-duced luminol luminescence. J. Am. Chem. Soc. 118, 6619-6624 (1996).
6. H. J. Sipe, Jr., J. T. Corbett and R. P. Mason, In vitro free radical metabolism of phenolphthalein by peroxidases. Drug Metab. Dispos. 25, 468-480 (1997).
7. J. P. Crow, Dichlorofluorescein and dihydrorhodamine 123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurements of reactive nitrogen and oxygen species. Nitric Oxide 1, 145-157 (1997).
8. Y. R. Chen, L. J. Deterding, B. E. Sturgeon, K. B. Tomer and R. P. Mason, Protein oxidation of cytochrome c by reactive halogen species enhances its peroxidase activity. J. Biol. Chem. 277, 29781-29791 (2002).
9. R. G. Keynes, C. Griffiths and J. Garthwaite, Superoxide-dependent consumption of nitric oxide in biological media may confound in vitro experiments. Biochem. J. 369, 399-406 (2003).
10. J. K. Leach, S. M. Black, R. K. Schmidt-Ullrich and R. B. Mikkelsen, Activation of constitutive nitric-oxide synthase activity is an early signaling event induced by ionizing radiation. J. Biol. Chem. 277, 15400-15406 (2002).