Radiation-induced long-lived extracellular radicals do not contribute to measurement of intracellular reactive oxygen species using the dichlorofluorescein method

Radiation Research

Volumn 169, Issue 4, 2008, Pages 469-473

  Hafer, Kurt ^a,b   Konishi, Teruaki ^b   Schiestl, Robert H ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DICHLOROFLUORESCEIN; HYDROGEN PEROXIDE; RADIATION INDUCED LONG LIVED EXTRACELLULAR RADICAL; RADICAL; REACTIVE OXYGEN METABOLITE;

ANALYTIC METHOD; ANIMAL CELL; ARTICLE; DICHLOROFLUORESCEIN METHOD; FEMALE; FLOW CYTOMETRY; FLUORESCENCE; IONIZING RADIATION; NONHUMAN; PRIORITY JOURNAL; RADIATION EXPOSURE; RADIATION RESPONSE; RADIOLYSIS;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; FLUORESCEINS; FREE RADICALS; OXIDATION-REDUCTION; REACTIVE OXYGEN SPECIES;

EID: 42949101460     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR1211.1     Document Type: Article

References (29)

1. J. P. Pouget and S. J. Mather, General aspects of the cellular response to low- and high-LET radiation. Eur. J. Nucl. Med. 28, 541-561 (2001).
2. N. Soh, Recent advances in fluorescent probes for the detection of reactive oxygen species. Anal. Bioanal. Chem. 386, 532-543 (2006).
3. H. Yang, N. Asaad and K. D. Held, Medium-mediated intercellular communication is involved in bystander responses of X-ray-irradiated normal human fibroblasts. Oncogene 24, 2096-2103 (2005).
4. J. K. Leach, G. Van Tuyle, P. S. Lin, R. Schmidt-Ullrich and R. B. Mikkelsen, Ionizing radiation-induced, mitochondria-dependent generation of reactive oxygen/nitrogen. Cancer Res. 61, 3894-3901 (2001).
5. Y. Takada, M. Hachiya, S. H. Park, Y. Osawa, T. Ozawa and M. Akashi, Role of reactive oxygen species in cells overexpressing manganese superoxide dismutase: mechanism for induction of radioresistance. Mol. Cancer Res. 1, 137-146 (2002).
6. X. S. Wan, Z. Zhou and A. R. Kennedy, Adaptation of the dichlorofluorescein assay for detection of radiation-induced oxidative stress in cultured cells. Radiat. Res. 160, 622-630 (2003).
7. X. S. Wan, Z. Zhou, J. H. Ware and A. R. Kennedy, Standardization of a fluorometric assay for measuring oxidative stress in irradiated cells. Radiat. Res. 163, 232-240 (2005).
8. X. S. Wan, P. Bloch, J. H. Ware, Z. Zhou, J. J. Donahue, J. Guan, J. Stewart and A. R. Kennedy, Detection of oxidative stress induced by low- and high-linear energy transfer radiation in cultured human epithelial cells. Radiat. Res. 163, 364-368 (2005).
9. X. S. Wan, J. H. Ware, Z. Zhou, J. J. Donahue, J. Guan and A. R. Kennedy, Protection against radiation-induced oxidative stress in cultured human epithelial cells by treatment with antioxidant agents. Int. J. Radiat. Oncol. Biol. Phys. 64, 1475-1481 (2006).
10. Y. N. Korystov, V. V. Shaposhnikova, A. F. Korystova and M. O. Emel'yanov, Detection of reactive oxygen species induced by radiation in cells using the dichlorofluorescein assay. Radiat. Res. 168, 226-232 (2007).
11. 2 in cultured endothelial cells. Arch. Biochem. Biophys. 302, 348-355 (1993).
12. B. Pastina and J. A. LaVerne, Hydrogen peroxide production in the radiolysis of water with heavy ions. J. Phys. Chem. A 103, 1592-1597 (1999).
13. E. Sierra-Rivera, G. J. Voorhees and M. L. Freeman, Gamma irradiation increases hsp-70 in Chinese hamster ovary cells. Radiat. Res. 135, 40-45 (1993).
14. D. A. Bass, J. W. Parce, L. R. Dechatelet, P. Szejda, M. C. Seeds and M. Thomas, Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation. J. Immunol. 130, 1910-1917 (1983).
15. E. J. Hart, Radiation chemistry of aqueous solutions. Annu. Rev. Nucl. Sci. 15, 125-150 (1965).
16. M. Tubiana, J. Dutreix and A. Wambersie, Introduction to Radiobiology. Taylor & Francis, London, 1990.
17. M. Saran and W. Bors, Radiation chemistry of physiological saline reinvestigated: Evidence that chloride-derived intermediates play a key role in cytotoxicity. Radiat. Res. 147, 70-77 (1997).
18. H. Zhu, G. L. Bannenberg, P. Moldeus and H. G. Schertzer, Oxidation pathways for the intracellular probe 2′,7′-dichlorofluorescein. Arch. Toxicol. 68, 582-587 (1994).
19. 2-photosensitized ROS generation in cultured human skin fibroblasts. Photochem. Photobiol. 82, 5-12 (2006).
20. W. Jakubowski and G. Bartosz, Estimation of oxidative stress in Saccharomyces cerevisae with fluorescent probes. Int. J. Biochem. Cell Biol. 29, 1297-1301 (1997).
21. S. Gus-Mayer, B. Naton, K. Hahlbrock and E. Schmelzer, Local mechanical stimulation induces components of the pathogen defense response in parsley. Proc. Natl. Acad. Sci. USA 95, 8398-8403 (1998).
22. H. Lu and V. J. Higgins, Measurement of active oxygen species generated in planta in response to elicitor AVR9 of ladosporium fulvum. Physiol. Mol. Plant Pathol. 52, 35-51 (1998).
23. A. D. Shapiro and C. Zhang, The role of NDR1 in avirulence gene-directed signaling and control of programmed cell death in Arabidopsis. Plant Physiol. 127, 1089-1101 (2001).
24. P. Ubezio and F. Civoli, Flow cytometric detection of hydrogen peroxide production induced by doxorubicin in cancer cells. Free Radic. Biol. Med. 16, 509-516 (1994).
25. G. A. Sawada, T. J. Raub, D. E. Decker and S. E. Buxser, Analytical and numerical techniques for the evaluation of free radical damage in cultured cells using scanning laser microscopy. Cytometry 25, 254-262 (1996).
26. K. E. Chapman, S. E. Sinclair, D. Zhuang, A. Hassid, L. P. Desai and C. M. Waters, Cyclic mechanical strain increases reactive oxygen species production in pulmonary epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 289, 834-841 (2005).
27. P. Maher and A. Hanneken, The molecular basis of oxidative stress-induced cell death in an immortalized retinal ganglion cell line. Invest. Ophthalmol. Vis. Sci. 46, 749-757 (2005).
28. C. L. Murrant, F. H. Andrade and M. B. Reid, Exogenous reactive oxygen and nitric oxide alter intracellular oxidant status of skeletal muscle fibres. Acta Physiol. Scand. 166, 111-121 (1999).
29. G. Freyburger and S. Labrouche, Flow cytometry assessment of leukocyte functions in vascular pathologies. Hematol. Cell Ther. 38, 513-526 (1997).