Comparison of SYPRO Ruby and Deep Purple using commonly available UV transilluminator: Wide-scale application in proteomic research

Journal of Proteome Research

Volumn 7, Issue 7, 2008, Pages 2797-2802

  Chakravarti, Bulbul ^a   Ratanaprayul, Wongrat ^a   Dalal, Neville ^a   Chakravarti, Deb N ^a  

^a KECK GRADUATE INSTITUTE   (United States)

Author keywords

Deep purple; Proteomics; SYPRO ruby

Indexed keywords

AMINO ACID; DEEP PURPLE; FLUORESCENT DYE; SYPRO RUBY; UNCLASSIFIED DRUG; ORGANOMETALLIC COMPOUND; PROTEOME;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; IMAGING SYSTEM; INTERMETHOD COMPARISON; LIQUID CHROMATOGRAPHY; LIVER HOMOGENATE; MALE; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; TANDEM MASS SPECTROMETRY; ULTRAVIOLET RADIATION; ANIMAL; C57BL MOUSE; CHEMISTRY; COMPARATIVE STUDY; LIVER; METHODOLOGY; SENSITIVITY AND SPECIFICITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FLUORESCENT DYES; LIVER; MALE; MICE; MICE, INBRED C57BL; ORGANOMETALLIC COMPOUNDS; PROTEOME; PROTEOMICS; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; ULTRAVIOLET RAYS;

EID: 51549122099     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr7007225     Document Type: Article

References (12)

1. Patton, W. F. A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics. Electrophoresis 2000, 21, 1123-1144.
2. Patton, W. F. Detection technologies in proteome analysis. J. Chromatogr., B: Anal. Technol. Biomed. Life Sci. 2002, 771, 3-31.
3. Lopez, M. F.; Mikulskis, A.; Golenko, E.; Herick, K.; Spibey, C. A.; Taylor, I.; Bobrow, M.; Jackson, P. High-content proteomics: fluorescence multiplexing using an integrated, high-sensitivity, multiwavelength charge-coupled device imaging system. Proteomics 2003, 3, 1109-1116.
4. Hart, C;.; Schulenberg, B.; Steinberg, T. H.; Leung, W. Y.; Patton, W. F. Detection of glycoproteins in polyacrylamide gels and on electroblots using Pro-Q Emerald 488 dye, a fluorescent periodate Schiff-base stain. Electrophoresis 2003, 24, 588-598.
5. Adam, G. C.; Sorensen, E. J.; Cravatt, B. F. Chemical strategies for functional proteomics. Mol. Cell. Proteomics 2002, 1, 781-790.
6. Adam, G. C; Sorensen, E. J.; Cravatt, B. F. Trifunctional chemical probes for the consolidated detection and identification of enzyme activities from complex proteomes. Mol. Cell. Proteomics 2002, 1, 828-835.
7. Steinberg, T. H.; Agnew, B. J.; Gee, K. R.; Leung, W. Y.; Goodman, T.; Schulenberg, B.; Hendrickson, J.; Beechem, J. M.; Haugland, R. P.; Patton, W. F. Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology. Proteomics 2003, 3, 1128-1144.
8. Chakravarti, B.; Oseguera, M.; Dalai, N.; Fathy, P.; Mallik, B.; Raval, A.; Chakravarti, D. N. Proteomic profiling of aging in the mouse heart: Altered expression of mitochondrial proteins. Arch. Biochem. Biophys. 2008, in press.
9. Comparison of Deep Purple Total Protein Stain and Sypro Ruby in 1-D and 2-D gel electrophoresis, Application note, 2003, Amersham Biosciences, 18-1177-44, 2003-09, pp 1-5.
10. Hart, C; Ahnert, N.; Hajivandi, M.; Lindsey, A.; Harwood, S. H. Comparative performance of fluorescent total protein stains on one- and two- dimensional PAGE gels. J. Biomol. Tech. 2006, 17, 5.
11. Harris, L. R.; Churchward, M. A.; Butt, R. H.; Coorsen, J. R. Assessing detection of methods for gel-based proteomic analyses. J. Proteome Res. 2007, 6, 1418-1425.
12. Tannu, N. S.; Sanchez-Brambila, G.; Kirby, P.; Andacht, T. M. Effect of staining reagent on peptide mass fingerprinting from in-gel trypsin digestions: A comparison of SyproRuby TM and DeepPur-pleTM. Electrophoresis 2006, 27, 3136-3143.