Effect of staining reagent on peptide mass fingerprinting from in-gel trypsin digestions: A comparison of SyproRuby™ and DeepPurple™

Electrophoresis

Volumn 27, Issue 15, 2006, Pages 3136-3143

  Tannu, Nilesh S ^a,b   Sanchez Brambila, Gabriela ^a   Kirby, Pam ^a   Andacht, Tracy M ^a  

^a UNIVERSITY OF GEORGIA   (United States)

^b WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

DeepPurple ; Mass spectrometry; SyproRuby ; Two dimensional gel

Indexed keywords

AMINO ACIDS; BINDING SITES; PEPTIDES;

DEEPPURPLE™; DOWN-STREAM; GEL METHOD; PEPTIDE MASS FINGERPRINTING; PROTEIN QUANTITATIONS; PROTEOME ANALYSIS; REPRODUCIBILITIES; SYPRORUBY™; TRYPSIN DIGESTION; TWO-DIMENSIONAL GELS;

MASS SPECTROMETRY;

PROTEOME; REAGENT; TRYPSIN;

ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; DNA FINGERPRINTING; DOWNSTREAM PROCESSING; FEASIBILITY STUDY; FLUORESCENCE ANALYSIS; MASS SPECTROMETRY; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 33747745348     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200500740     Document Type: Article

References (22)

1. Neuhoff, V., Stamm, R., Pardowitz, I., Arnold, N. et al., Electrophoresis 1990, 11, 101-117.
2. Scheler, C., Lamer, S., Pan, Z., Li, X.-P. et al., Electrophoresis 1998, 19, 918-927.
3. Mortz, E., Krogh, T. N., Vorum, H., Görg, A. et al., Proteomics 2001, 1, 1359-1363.
4. White, I. R., Pickford, R., Wood, J., Skehel, J. M. et al., Electrophoresis 2004, 25, 3048-3054.
5. Berggren, K., Steinberg, T. H., Lauber, W. M., Carroll, J. A. et al., Anal. Biochem. 1999, 276, 129-143.
6. Berggren, K., Chernokalskaya, E., Steinberg, T. H., Kemper, C. et al., Electrophoresis 2000, 21, 2509-2521.
7. Lopez, M. F., Berggren, K., Chernokalskaya, E., Lazarev, A. et al., Electrophoresis 2000, 21, 3673-3683.
8. Smejkal, G. B., Robinson, M. H., Lazarev, A., Electrophoresis 2004, 25, 2511-2519.
9. Mackintosh, J. A., Choi, H.-Y., Bae, S.-H., Veal, D. A. et al., Proteomics 2003, 3, 2273-2288.
10. Bjellqvist, B., Ek, K., Righetti, P. G., Gianazza, E. et al., J. Biochem. Biophys. Methods 1982, 6, 317-339.
11. Tannu, N. S., Wu, J., Rao, V. K., Gadgil, H. S. et al., Anal. Biochem. 2004, 327, 222-232.
12. O'Farrell, P. H., J. Biol. Chem. 1975, 250, 4007-4021.
13. Wu, J., Lenchik, N. J., Pabst, M. J., Solomon, S. S. et al., Electrophoresis 2005, 26, 225-237.
14. Swatton, J. E., Prabakaran, S., Karp, N. A., Lilley, K. S., Bahn, S. et al., Mol. Psychiatry 2004, 9, 128-143.
15. Tannu, N. S., Rao, V. K., Chaudhary, R. M., Giorgianni, F. et al., Mol. Cell. Proteomics 2004, 3, 1065-1082.
16. Henzel, W. J., Billeci, T. M., Stults, J. T., Wong, S. C. et al., Proc. Natl. Acad. Sci. USA 1993, 90, 5011-5015.
17. Patterson, S. D., Aebersold, R., Electrophoresis 1995, 16, 1791-1814.
18. Lanne, B., Panfilov, O., J. Proteome Res. 2005, 4, 175-179.
19. Coghlan, D. R., Mackintosh, J. A., Karuso, P., Org. Lett. 2005, 7, 2401-2404.
20. Terry, D. E., Umstot, E., Desiderio, D. M., J. Am. Soc. Mass Spectrom. 2004, 15, 784-294.
21. Finehout, E. J., Cantor, J. R., Lee, K. H., Proteomics 2005, 5, 2319-2321.
22. Hale, J. E., Butler, J. P., Gelfanova, V., You, J. S., Knierman, M. D., Anal. Biochem. 2004, 333, 174-181.