A ketoreductase domain in the PksJ protein of the bacillaene assembly line carries out both α- and β-ketone reduction during chain growth

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 35, 2008, Pages 12809-12814

  Calderone, Christopher T ^a,c   Bumpus, Stefanie B ^b   Kelleher, Neil L ^b   Walsh, Christopher T ^a   Magarvey, Nathan A ^a,d  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c MACALESTER COLLEGE   (United States)

^d MCMASTER UNIVERSITY   (Canada)

Author keywords

Biosynthesis; Enzymology; Natural product; Polyketide

Indexed keywords

2 OXOISOCAPROIC ACID; ALPHA HYDROXYISOCAPROATE; ALPHA KETONE; BACILLAENE; BETA KETONE; DIHYDROBACILLAENE; GLYCINE; HEXANOIC ACID DERIVATIVE; HYBRID PROTEIN; KETONE DERIVATIVE; KETOREDUCTASE; NONRIBOSOMAL PEPTIDE SYNTHETASE; OXIDOREDUCTASE; PEPTIDE SYNTHASE; PKSJ PROTEIN; POLYKETIDE; POLYKETIDE SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; MASS SPECTROMETRY; METABOLITE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FUNCTION;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESTERS; KETO ACIDS; KETONES; OXIDATION-REDUCTION; POLYENES; PROTEIN STRUCTURE, TERTIARY; STRUCTURE-ACTIVITY RELATIONSHIP;

BACILLUS SUBTILIS;

EID: 51349154318     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0806305105     Document Type: Article

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