S-adenosylhomocysteine hydrolase overexpression in HEK-293 cells: Effect on intracellular adenosine levels, cell viability, and DNA methylation

Cellular Physiology and Biochemistry

Volumn 22, Issue 1-4, 2008, Pages 223-236

  Hermes, Marina ^a   Osswald, Hartmut ^a   Riehle, Renate ^a   Piesch, Christine ^a   Kloor, Doris ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Adenosine; Apoptosis; DNA methylation; Overexpression; S Adenosylhomocysteine hydrolase

Indexed keywords

ADENOSINE; ADENOSYLHOMOCYSTEINASE; CASPASE;

APOPTOSIS; ARTICLE; CATALYSIS; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; DNA METHYLATION; ENERGY METABOLISM; ENZYME ACTIVITY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; HYDROLYSIS; PRIORITY JOURNAL;

EID: 51149107642     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000149800     Document Type: Article

References (40)

1. de la Haba G, Cantoni G: The enzymatic synthesis of S-adenosyl-L- homocysteine from adenosine and homocysteine. J Biol Chem 1959;234:606-608.
2. Clarke S, Banfield K: S-Adenosylmethionine-dependent Methyltransferases; in Carmel R and Jacobsen D (eds): Homocysteine in Health and Disease. Cambridge University Press, 2001, pp. 63-78.
3. Hermes M, Osswald H, Mattar J, Kloor D: Influence of an altered methylation potential on mRNA methylation and gene expression in HepG2 cells. Exp Cell Res 2004;294:325-334.
4. Kramer K, Harrington EO, Lu Q, Bellas R, Newton J, Sheahan KL, Rounds S: Isoprenylcysteine Carboxyl Methyltransferase Activity Modulates Endothelial Cell Apoptosis. Mol Biol Cell 2003;14:848-857.
5. Yi P, Melnyk S, Pogribna M, Pogribny IP, Hine RJ, James SJ: Increase in Plasma Homocysteine Associated with Parallel Increases in Plasma S-Adenosylhomocysteine and Lymphocyte DNA Hypomethylation. J Biol Chem 2000;275:29318-29323.
6. Kloor D, Hermes M, Fink K, Schmid H, Klingel K, Mack A, Grenz A, Osswald H: Expression and localization of S-adenosylhomocysteine-hydrolase in the rat kidney following carbon monoxide induced hypoxia. Cell Physiol Biochem 2007;19:57-66.
7. Radomski N, Barreto G, Kaufmann C, Yokoska J, Mizumoto K, Dreyer C: Interaction of S-adenosylhomocysteine hydrolase of Xenopus laevis with mRNA(guanine-7-)methyltransferase: implication on its nuclear compartmentalisation and on cap methylation of hnRNA. Biochim Biophys Acta 2002;1590:93-102.
8. Cantoni G, Chiang P: The role of S-adenosylhomocysteine and S-adenosylhomocysteine hydrolase in the control of biological methylations; in Cavalli D, Gaull G, and Zappia V (eds): Natural sulfur compounds: novel biochemical and structural aspects. New York, Plenum Press, 1980, pp. 67-80.
9. Dawicki DD, Chatterjee D, Wyche J, Rounds S: Extracellular ATP and adenosine cause apoptosis of pulmonary artery endothelial cells. Am J Physiol 1997;273:L485-L494.
10. Hermes M, Osswald H, Kloor D: Role of S-adenosylhomocysteine hydrolase in adenosine-induced apoptosis in HepG2 cells. Exp Cell Res 2007;313:264-283.
11. Rounds S, Yee WL, Dawicki DD, Harrington E, Parks N, Cutaia MV: Mechanism of extracellular ATP- and adenosine-induced apoptosis of cultured pulmonary artery endothelial cells. Am J Physiol 1998;275:L379-L388.
12. Blay J, White TD, Hoskin DW: The extracellular fluid of solid carcinomas contains immunosuppressive concentrations of adenosine. Cancer Res 1997;57:2602-2605.
13. Kloor D, Delabar U, Muehlbauer B, Luippold G, Osswald H: Tissue levels of S-adenosylhomocysteine in the rat kidney: effects of ischemia and homocysteine. Biochem Pharmacol 2002;63:809-815.
14. Finkelstein J: Pathways and regulation of homocysteine metabolism in mammals. Semin Thromb Hemost 2000;26:219-225.
15. Medina M, Urdiales JL, Amores-Sanchez MI: Roles of homocysteine in cell metabolism: old and new functions. Eur J Biochem 2001;268:3871-3882.
16. Hoffman DR, Marion DW, Cornatzer WE, Duerre JA: S-Adenosylmethionine and S-adenosylhomocystein metabolism in isolated rat liver. Effects of L-methionine, L-homocystein, and adenosine. J Biol Chem 1980;255:10822-10827.
17. Loscalzo J: Homocysteine trials-clear outcomes for complex reasons. N Engl J Med 2006;354:1629-1632.
18. Miller MW, Duhl DM, Winkes BM, Arredondo-Vega F, Saxon PJ, Wolff GL, Epstein CJ, Hershfield MS, Barsh GS: The mouse lethal nonagouti (a(x)) mutation deletes the S-adenosylhomocysteine hydrolase (Ahcy) gene. EMBO J 1994;13:1806-1816.
19. Baric I, Fumic K, Glenn B, Cuk M, Schulze A, Finkelstein JD, James SJ, Mejaski-Bosnjak V, Pazanin L, Pogribny IP, Rados M, Sarnavka V, Scukanec-Spoljar M, Allen RH, Stabler S, Uzelac L, Vugrek O, Wagner C, Zeisel S, Mudd SH: S-adenosylhomocysteine hydrolase deficiency in a human: A genetic disorder of methionine metabolism. Proc Natl Acad Sci U S A 2004;101:4234-4239.
20. Schaade L, Kleines M, Krone B, Hausding M, Walter R, Ritter K: Enhanced transcription of the S-adenosylhomocysteine hydrolase gene precedes Epstein-Barr virus lytic gene activation in ganglioside-stimulated lymphoma cells. Med Microbiol Immunol (Berl) 2000;189:13-18.
21. Hermes M, Hippel von S, Osswald H, Kloor D: S-Adenosylhomocysteine Metabolism in Different Cell Lines: Effect of Hypoxia and Cell Density. Cell Physiol Biochem 2005;15:233-244.
22. Ault-Riche DB, Yuan CS, Borchardt RT: A single mutation at lysine 426 of human placental S- adenosylhomocysteine hydrolase inactivates the enzyme. J Biol Chem 1994;269:31472-31478.
23. Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucl Acids Res 2001;29:e45.
24. Kloor D, Stumvoll W, Schmid H, Koempf J, Mack A, Osswald H: Localization of S-adenosylhomocysteine hydrolase in the rat kidney. J Histochem Cytochem 2000;48:211-218.
25. Neumeister B, Faigle M, Lauber K, Northoff H, Wesselborg S: Legionella pneumophila induces apoptosis via the mitochondrial death pathway. Microbiology 2002;148:3639-3650.
26. Pogribny I, Yi P, James SJ: A Sensitive New Method for Rapid Detection of Abnormal Methylation Patterns in Global DNA and within CpG Islands. Biochem Biophys Res Commun 1999;262:624-628.
27. Atkinson DE, Walton GM: Adenosine Triphosphate Conservation in Metabolic Regulation. Rat Liver Citrate Cleavage Enzyme. J Biol Chem 1967;242:3239-3241.
28. Villanueva JA, Halsted CH: Hepatic transmethylation reactions in micropigs with alcoholic liver disease. Hepatology 2004;39:1303-1310.
29. Stockand JD, Al Baldawi NF, Al Khalili OK, Worrell RT, Eaton DC: S-Adenosyl-L-homocysteine Hydrolase Regulates Aldosterone-induced Na+ Transport. J Biol Chem 1999;274:3842-3850.
30. Kloor D, Kurz J, Fuchs S, Faust B, Osswald H: S-adenosylhomocysteine- hydrolase from bovine kidney: enzymatic and binding properties. Kidney Blood Press Res 1996;19:100-108.
31. Deussen A, Borst M, Schrader J: Formation of S-adenosylhomocysteine in the heart. I: An index of free intracellular adenosine. Circ Res 1988;63:240-249.
32. Kloor D, Osswald H: S-Adenosylhomocysteine hydrolase as a target for intracellular adenosine action. Trends Pharmacol Sci 2004;25:294-297.
33. Veech RL, Lawson JW, Cornell NW, Krebs HA: Cytosolic phosphorylation potential. J Biol Chem 1979;254:6538-6547.
34. Barrette WC Jr, Hannum DM, Wheeler WD, Hurst JK: Viability and metabolic capability are maintained by Escherichia coli, Pseudomonas aeruginosa, and Streptococcus lactis at very low adenylate energy charge. J Bacteriol 1988;170:3655-3659.
35. Aldrich MB, Chen W, Blackburn MR, Martinez-Valdez H, Datta SK, Kellems RE: Impaired germinal center maturation in adenosine deaminase deficiency. J Immunol 2003;171:5562-5570.
36. Hermes M, Osswald H, Kloor D: Adenosine metabolism and its effect on methylation potential in cultured cells: methodological considerations. Cell Mol Biol (Noisy-le-grand) 2007;52:OL874-OL881.
37. Slee EA, Adrain C, Martin SJ: Serial killers: ordering caspase activation events in apoptosis. Cell Death Differ 1999;6:1067-1074.
38. Troy CM, Shelanski ML: Caspase-2 redux. Cell Death Differ 2003;10: 101-107.
39. James SJ, Melnyk S, Pogribna M, Pogribny IP, Caudill MA: Elevation in S-Adenosylhomocysteine and DNA Hypomethylation: Potential Epigenetic Mechanism for Homocysteine-Related Pathology. J Nutr 2002;132:2361S-2366S.
40. Vertino PM, Yen RW, Gao J, Baylin SB: De novo methylation of CpG island sequences in human fibroblasts overexpressing DNA (cytosine-5-)- methyltransferase. Mol Cell Biol 1996;16:4555-4565.