Biological enrichment of mycoplasma agents by cocultivation with permissive cell cultures

Applied and Environmental Microbiology

Volumn 74, Issue 17, 2008, Pages 5383-5391

  Volokhov, Dmitriy V ^a   Kong, Hyesuk ^a   George, Joseph ^a   Anderson, Christine ^a   Chizhikov, Vladimir E ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDS; ANIMAL CELL CULTURE; BLAST ENRICHMENT; CELL GROWTH; CELLS; CONTAMINATION; CYTOLOGY; INDUSTRIAL RESEARCH; MAMMALS; NUCLEIC ACIDS; POLLUTION DETECTION; RESEARCH LABORATORIES; SAFETY TESTING; SUBSTRATES;

CELL LINES; CELL SUBSTRATES; MYCOPLASMA SPECIES;

CELL CULTURE;

BACTERIUM; CYTOLOGY; DRUG; GROWTH RESPONSE; NUCLEIC ACID; TEMPERATURE EFFECT; TESTING METHOD;

ACHOLEPLASMA LAIDLAWII; ANIMAL CELL; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; CELL CULTURE; CELL STRAIN; CELL STRAIN HEK293; CELL STRAIN HEPG2; COCULTURE; CONTROLLED STUDY; HUMAN; HUMAN CELL; INSECT CELL CULTURE; MAMMAL CELL; MYCOPLASMA; MYCOPLASMA ARGININE; MYCOPLASMA BOVIS; MYCOPLASMA FERMENTANS; MYCOPLASMA GALLINACEUM; MYCOPLASMA GALLISEPTICUM; MYCOPLASMA HOMINIS; MYCOPLASMA HYORHINIS; MYCOPLASMA ORALE; MYCOPLASMA SALIVARIUM; NONHUMAN; TEMPERATURE DEPENDENCE; VERO CELL;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CELL CULTURE TECHNIQUES; CELL LINE; CERCOPITHECUS AETHIOPS; COCULTURE TECHNIQUES; DNA, BACTERIAL; DOGS; INSECTS; MYCOPLASMA; MYCOPLASMA INFECTIONS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VERO CELLS;

ACHOLEPLASMA LAIDLAWII; BACTERIA (MICROORGANISMS); HEXAPODA; MAMMALIA; MYCOPLASMA; MYCOPLASMA ARGININI; MYCOPLASMA BOVIS; MYCOPLASMA FERMENTANS; MYCOPLASMA GALLINACEUM; MYCOPLASMA GALLISEPTICUM; MYCOPLASMA HOMINIS; MYCOPLASMA HYORHINIS; MYCOPLASMA ORALE; MYCOPLASMA SALIVARIUM; MYCOPLASMA SYNOVIAE; MYCOPLASMATALES;

EID: 50949123793     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.00720-08     Document Type: Article

References (68)

1. Abele-Horn, M., U. Busch, H. Nitschko, E. Jacobs, R. Bax, F. Pfaff, B. Schaffer, and J. Heesemann. 1998. Molecular approaches to diagnosis of pulmonary diseases due to Mycoplasma pneumoniae. J. Clin. Microbiol. 36:548-551.
2. Aldridge, K. E. 1975. Growth and cytopathology of Mycoplasma synoviae in chicken embryo cell cultures. Infect. Immun. 12:198-204.
3. Baird, S. C., J. Carman, R. P. Dinsmore, R. L. Walker, and J. K. Collins. 1999. Detection and identification of Mycoplasma from bovine mastitis infections using a nested polymerase chain reaction. J. Vet. Diagn. Investig. 11:432-435.
4. Benisheva, T., V. Sovova, I. Ivanov, and G. Opalchenova. 1993. Comparison of methods used for detection of mycoplasma contamination in cell cultures, sera, and live-virus vaccines. Folia Biol. (Praha) 39:270-276.
5. Benton, W. J., M. S. Cover, and F. W. Melchior. 1967. Mycoplasma gallisepticum in a commercial laryngotracheitis vaccine. Avian Dis. 11:426-429.
6. Boatman, E. S., and G. E. Kenny. 1971. Morphology and ultrastructure of Mycoplasma pneumoniae spherules. J. Bacteriol. 106:1005-1015.
7. Bota, D. A., J. K. Ngo, and K. J. Davies. 2005. Downregulation of the human Lon protease impairs mitochondrial structure and function and causes cell death. Free Radic. Biol. Med. 38:665-677.
8. Brown, D. R. 2002. Mycoplasmosis and immunity of fish and reptiles. Front. Biosci. 7:d1338-d1346.
9. Cho, M. J., D. P. Thompson, C. T. Cramer, T. J. Vidmar, and J. F. Scieszka. 1989. The Madin Darby canine kidney (MDCK) epithelial cell monolayer as a model cellular transport barrier. Pharm. Res. 6:71-77.
10. Darai, G., R. M. Flugel, L. Zoller, B. Matz, A. Kreig, H. Gelderblom, H. Delius, and R. H. Leach. 1981. The plaque-forming factor for mink lung cells present in cytomegalovirus and herpes-zoster virus stocks identified as Mycoplasma hyorhinis. J. Gen. Virol. 55:201-205.
11. Darai, G., L. Zoller, R. M. Flugel, and H. Gelderblom. 1983. Mink lung cells as a tool for detection of Mycoplasma hyorhinis contamination in cell cultures and virus stocks. In Vitro 19:7-15.
12. Del Giudice, R. A. 1998. M-CMRL, a new axenic medium to replace indicator cell cultures for the isolation of all strains of Mycoplasma hyorhinis. In Vitro Cell Dev. Biol. Anim. 34:88-89.
13. Del Giudice, R. A., R. S. Gardella, and H. E. Hopps. 1980. Cultivation of formerly noncultivable strains of Mycoplasma hyorhinis. Curr. Microbiol. 4:75-80.
14. Drexler, H. G., C. C. Uphoff, W. G. Dirks, and R. A. MacLeod. 2002. Mix-ups and mycoplasma: the enemies within. Leukoc. Res. 26:329-333.
15. Eldering, J. A., C. Felten, C. A. Veilleux, and B. J. Potts. 2004. Development of a PCR method for mycoplasma testing of Chinese hamster ovary cell cultures used in the manufacture of recombinant therapeutic proteins. Biologicals 32:183-193.
16. Fadiel, A., K. D. Eichenbaum, N. El Semary, and B. Epperson. 2007. Mycoplasma genomics: tailoring the genome for minimal life requirements through reductive evolution. Front. Biosci. 12:2020-2028.
17. Fraser, C. M., J. D. Gocayne, O. White, M. D. Adams, R. A. Clayton, R. D. Fleischmann, C. J. Bult, A. R. Kerlavage, G. Sutton, J. M. Kelley, R. D. Fritchman, J. F. Weidman, K. V. Small, M. Sandusky, J. Fuhrmann, D. Nguyen, T. R. Utterback, D. M. Saudek, C. A. Phillips, J. M. Merrick, J. F. Tomb, B. A. Dougherty, K. F. Bott, P. C. Hu, T. S. Lucier, S. N. Peterson, H. O. Smith, C. A. Hutchison III, and J. C. Venter. 1995. The minimal gene complement of Mycoplasma genitalium. Science 270:397-403.
18. Gardella, R. S., and R. A. Del Giudice. 1995. Growth of Mycoplasma hyorhinis cultivar alpha on semisynthetic medium. Appl. Environ. Microbiol. 61:1976-1979.
19. Geissdorfer, W., G. Sandner, S. John, A. Gessner, C. Schoerner, and K. Schroppel. 2008. Ureaplasma urealyticum meningitis in an adult patient. J. Clin. Microbiol. 46:1141-1143.
20. Gerbal, M., P. Fournier, P. Barry, M. Mariller, F. Odier, G. Devauchelle, and M. Duonor-Cerutti. 2000. Adaptation of an insect cell line of Spodoptera frugiperda to grow at 37 degrees C: characterization of an endodiploid clone. In Vitro Cell Dev. Biol. Anim. 36:117-124.
21. Gouriet, F., F. Fenollar, J. Y. Patrice, M. Drancourt, and D. Raoult. 2005. Use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience. J. Clin. Microbiol. 43:4993-5002.
22. Hackett, K. J., and D. E. Lynn. 1985. Cell-assisted growth of a fastidious Spiroplasma. Science 230:825-827.
23. Hackett, K. J., D. E. Lynn, A. S. Ginsberg, S. Rottem, R. B. Henegar, J. Adams, D. L. Williamson, and R. F. Whitcomb. 1987. Cell-assisted culture of fastidious spiroplasmas: initial analysis of growth factors. Isr. J. Med. Sci. 23:667-670.
24. Hackett, K. J., D. E. Lynn, D. L. Williamson, A. S. Ginsberg, and R. F. Whitcomb. 1986. Cultivation of the Drosophila sex-ratio spiroplasma. Science 232:1253-1255.
25. Hamasuna, R., Y. Osada, and J. S. Jensen. 2005. Antibiotic susceptibility testing of Mycoplasma genitalium by TaqMan 5′ nuclease real-time PCR. Antimicrob. Agents Chemother. 49:4993-4998.
26. Hamasuna, R., Y. Osada, and J. S. Jensen. 2007. Isolation of Mycoplasma genitalium from first-void urine specimens by coculture with Vero cells. J. Clin. Microbiol. 45:847-850.
27. Hayes, M. M., H. H. Foo, H. Kotani, D. J. Wear, and S. C. Lo. 1993. In vitro antibiotic susceptibility testing of different strains of Mycoplasma fermentans isolated from a variety of sources. Antimicrob. Agents Chemother. 37:2500-2503.
28. Hlinak, P., J. Friedemann, and G. Starke. 1969. Problems of the state examination of viral vaccines in Mycoplasma contaminations. Pharmazie 24:189-192.
29. Hopps, H. E., and R. A. Del Giudice. 1984. Cell culture models as ancillary tools in the isolation and characterization of mycoplasmas. Isr. J. Med. Sci. 20:927-930.
30. Ikoev, V. N., G. M. Gonskii, and S. G. Dzagurov. 1973. A method of ultra-centrifugation used for control of live viral vaccines with regard to removal of mycoplasma. Vopr. Virusol. 18:625-628.
31. Ishikawa, Y., T. Kozakai, H. Morita, K. Saida, S. Oka, and Y. Masuo. 2006. Rapid detection of mycoplasma contamination in cell cultures using SYBR green-based real-time polymerase chain reaction. In Vilro Cell Dev. Biol. Anim. 42:63-69.
32. Jensen, J. S., H. T. Hansen, and K. Lind. 1996. Isolation of Mycoplasma genitalium strains from the male urethra. J. Clin. Microbiol. 34:286-291.
33. Jules Mattes, M. 2004. Control of the mycoplasma epidemic. In Vitro Cell Dev. Biol. Anim. 40:253-254.
34. Kojima, A., T. Takahashi, M. Kijima, Y. Ogikubo, M. Nishimura, S. Ntshimura, R. Harasawa, and Y. Tamura. 1997. Detection of Mycoplasma in avian live virus vaccines by polymerase chain reaction. Biologicals 25:365-371.
35. Kojima, A., T. Takahashi, M. Kijima, Y. Ogikubo, Y. Tamura, and R. Harasawa. 1996. Detection of mycoplasma DNA in veterinary live virus vaccines by the polymerase chain reaction. J. Vet. Med. Sci. 58:1045-1048.
36. Kong, H., D. V. Volokhov, J. George, P. Ikonomi, D. Chandler, C. Anderson, and V. Chizhikov. 2007. Application of cell culture enrichment for improving the sensitivity of mycoplasma detection methods based on nucleic acid amplification technology (NAT). Appl. Microbiol. Biotechnol. 77:223-232.
37. Krausse-Opatz, B., P. Dollmann, H. Zeidler, J. G. Kuipers, and L. Kohler. 2000. Frequent contamination of Chlamydia trachomatis and Chlamydia pneumoniae strains with mycoplasma. Biological relevance and selective eradication of mycoplasma from chlamydial cultures with mupirocin. Med. Microbiol. Immunol. (Berlin) 189:19-26.
38. Kuo, C. C., and J. T. Grayston. 1988. Factors affecting viability and growth in HcLa 229 cells of Chlamydia sp. strain TWAR. J. Clin. Microbiol. 26:812-815.
39. Lee, C. K. 1996. Issues of biological assays for viral vaccines. Dev. Biol. Stand. 88:41-47.
40. Levy, J. A., P. E. Sumner, and L. E. Hooser. 1982. Rapid tissue culture method for detection of Mycoplasma hyorhinis. J. Gen. Microbiol. 128:2817-2820.
41. Mackey, B. M., and C. M. Derrick. 1984. Conductance measurements of the lag phase of injured Salmonella typhimurium. J. Appl. Bacteriol. 57:299-308.
42. Madoff, S., B. Q. Pixley, R. A. DelGiudice, and R. C. Moellering, Jr. 1979. Isolation of Mycoplasma bovis from a patient with systemic illness. J. Clin. Microbiol. 9:709-711.
43. McGarrity, G. J., D. M. Phillips, and A. B. Vaidya. 1980. Mycoplasmal infection of lymphocyte cell cultures: infection with M. salivarium. In Vitro 16:346-356.
44. Nahapetian, A. T., J. N. Thomas, and W. G. Thilly. 1986. Optimization of environment for high density Vero cell culture: effect of dissolved oxygen and nutrient supply on cell growth and changes in metabolites. J. Cell Sci. 81:65-103.
45. Pitcher, D. G., and R. A. Nicholas. 2005. Mycoplasma host specificity: fact or fiction? Vet. J. 170:300-306.
46. Polster, U. 1986. Demonstration of Mycoplasma bovis as a contaminant in live virus vaccine. Arch. Exp. Veterinarmed. 40:147-150.
47. Prentice, M. J., and J. Farrant. 1977. Survival of chlamydiae after cooling to -196°C. J. Clin. Microbiol. 6:4-9.
48. Razin, S., and E. A. Freundt. 1984. The mycoplasmas, p. 740-775. In N. R. Krieg and J. G. Holt (ed.), Bergey's manual of systematic bacteriology, vol. 1. Williams & Wilkins, Baltimore, MD.
49. Razin, S., D. Yogev, and Y. Naot. 1998. Molecular biology and pathogenicity of mycoplasmas. Microbiol. Mol. Biol. Rev. 62:1094-1156.
50. Stakenborg, T., J. Vicca, P. Butaye, H. Imberechts, J. Peeters, A. De Kruif, F. Haesebrouck, and D. Maes. 2006. A multiplex PCR to identify porcine mycoplasmas present in broth cultures. Vet. Res. Commun. 30:239-247.
51. Steiner, T., and G. McGarrity. 1983. Mycoplasmal infection of insect cell cultures. In Vitro 19:672-682.
52. Steiner, T., G. J. McGarrity, J. M. Bove, D. M. Phillips, and M. Garnier. 1984. Insect cell cultures in the study of attachment and pathogenicity of spiroplasmas and mycoplasmas. Ann. Microbiol. (Paris) 135A:47-53.
53. Steiner, T., G. J. McGarrity, and D. M. Phillips. 1982. Cultivation and partial characterization of spiroplasmas in cell cultures. Infect. Immun. 35:296-304.
54. Sung, H., S. H. Kang, Y. J. Bae, J. T. Hong, Y. B. Chung, C. K. Lee, and S. Song. 2006. PCR-based detection of Mycoplasma species. J. Microbiol. 44:42-49.
55. Thornton, D. H. 1986. A survey of mycoplasma detection in veterinary vaccines. Vaccine 4:237-240.
56. Timenetsky, J., L. M. Santos, M. Buzinhani, and E. Mettifogo. 2006. Detection of multiple mycoplasma infection in cell cultures by PCR. Braz. J. Med. Biol. Res. 39:907-914.
57. Uphoff, C. C., and H. G. Drexler. 2005. Detection of mycoplasma contaminations. Methods Mol. Biol. 290:13-23.
58. Uphoff, C. C., and H. G. Drexler. 1999. Detection of mycoplasma contaminations in cell cultures by PCR analysis. Hum. Cell 12:229-236.
59. Uphoff, C. C., and H. G. Drexler. 2002. Detection of mycoplasma in leukemia-lymphoma cell lines using polymerase chain reaction. Leukemia 16:289-293.
60. Verkooyen, R. P., M. Sijmons, E. Fries, A. Van Belkum, and H. A. Verbrugh. 1997. Widely used, commercially available Chlamydia pneumoniae antigen contaminated with mycoplasma. J. Med. Microbiol. 46:419-424.
61. Volokhov, D. V., J. George, S. X. Liu, P. Ikonomi, C. Anderson, and V. Chizhikov. 2006. Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing. Appl. Microbiol. Biotechnol. 71:680-698.
62. Volokhov, D. V., A. A. Neverov, J. George, H. Kong, S. X. Liu, C. Anderson, M. K. Davidson, and V. Chizhikov. 2007. Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene. Mol. Phylogenet. Evol. 44:699-710.
63. Wang, H., F. Kong, P. Jelfs, G. James, and G. L. Gilbert. 2004. Simultaneous detection and identification of common cell culture contaminant and pathogenic Mollicutes strains by reverse line blot hybridization. Appl. Environ. Microbiol. 70:1483-1486.
64. Webster, D., H. Windsor, C. Ling, D. Windsor, and D. Pitcher. 2003. Chronic bronchitis in immunocompromised patients: association with a novel Mycoplasma species. Eur. J. Clin. Microbiol. Infect. Dis. 22:530-534.
65. Wellman, M. L., S. Krakowka, R. M. Jacobs, and G. J. Kociba. 1988. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell Dev. Biol. 24:223-229.
66. Woods, S. B. 1983. The isolation of a 'non-cultivable' strain of Mycoplasma hyorhinis from a mammalian live virus vaccine. J. Biol. Stand. 11:247-250.
67. Yagihashi, T., and K. Kato. 1984. Diversity in nicotinamide-adenine dinuscleotide requirement for the growth of different strains of Mycoplasma synoviae. Res. Vet. Sci. 37:353-354.
68. Zhang, P. F., M. Klutch, J. Muller, and C. J. Marcus-Sekura. 1994. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22:205-213.