Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives

Applied Microbiology and Biotechnology

Volumn 80, Issue 4, 2008, Pages 735-743

  Shibasaki, Seiji ^a   Sakata, Kozue ^b   Ishii, Jun ^c   Kondo, Akihiko ^c   Ueda, Mitsuyoshi ^d  

^a HYOGO COLLEGE OF MEDICINE   (Japan)

^b KOBE CITY COLLEGE OF TECHNOLOGY   (Japan)

^c KOBE UNIVERSITY   (Japan)

^d KYOTO UNIVERSITY   (Japan)

Author keywords

DHFR; PCA; Saccharomyces cerevisiae; Sulfanilamide; Trimethoprim

Indexed keywords

ASSAYS; CELL PROLIFERATION; CLONING; CONCENTRATION (PROCESS); DOPING (ADDITIVES); ENZYME ACTIVITY; FLOW INTERACTIONS; GENETIC ENGINEERING; ORGANIC ACIDS; PRINCIPAL COMPONENT ANALYSIS; THERMOMECHANICAL PULPING PROCESS;

BINDING DOMAIN; BINDING DOMAINS; COEXPRESSED; DHFR; DIHYDROFOLATE REDUCTASE; EXPERIMENTAL CONDITIONS; FOLIC ACID; FRAGMENT COMPLEMENTATION; IN-VITRO ASSAYS; IN-VIVO; ONCOPROTEIN; PCA; POSITIVE INTERACTION; PROKARYOTIC MICROORGANISMS; PROTEIN-PROTEIN INTERACTIONS; SACCHAROMYCES CEREVISIAE; SPECIFIC INHIBITORS; SULFANILAMIDE; TRIMETHOPRIM; WILD-TYPE; YEAST PROTEINS; YEAST SACCHAROMYCES CEREVISIAE;

YEAST;

DIHYDROFOLATE REDUCTASE; FOLIC ACID; GALACTOKINASE; MUTANT PROTEIN; ONCOPROTEIN; RAF PROTEIN; RAS PROTEIN; SULFANILAMIDE; TRIMETHOPRIM;

ANTIBIOTICS; ANTIMICROBIAL ACTIVITY; BIOASSAY; ENZYME ACTIVITY; EXPERIMENTAL STUDY; INHIBITOR; PROTEIN; YEAST;

ARTICLE; CELL PROLIFERATION; COMBINATORIAL LIBRARY; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FUNGAL STRAIN; GENE CONSTRUCT; GENE FUSION; GENE MUTATION; HOST; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN FRAGMENT COMPLEMENTATION ASSAY; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; WILD TYPE;

ENZYME INHIBITORS; GENE LIBRARY; GENETIC ENGINEERING; HUMANS; PLASMIDS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTO-ONCOGENE PROTEINS C-RAF; RAS PROTEINS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; TETRAHYDROFOLATE DEHYDROGENASE; TRIMETHOPRIM;

ADDITIVES; CLONES; CONCENTRATION; ENZYMES; GENETIC ENGINEERING; MICROORGANISMS; ORGANIC ACIDS; PROTEINS; SACCHAROMYCES CEREVISIAE; THERMOMECHANICAL PULPING; YEASTS;

EUKARYOTA; PROKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 50849120705     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1624-x     Document Type: Article

References (32)

1. SG Amyes 1982 Bactericidal activity of trimethoprim alone and in combination with sulfamethoxazole on susceptible and resistant Escherichia coli K-12 Antimicrob Agents Chemother 21 288 293
2. JR Appleman N Prendergast TJ Delcamp JH Freisheim RL Blakley 1988 Kinetics of the formation and isomerization of methotrexate complexes of recombinant human dihydrofolate reductase J Biol Chem 263 10304 10313
3. VH Brophy J Vasquez RG Nelson JR Forney A Rosowsky CH Sibley 2000 Identification of Cryptosporidium parvum dihydrofolate reductase inhibitors by complementation in Saccharomyces cerevisiae Antimicrob Agents Chemother 44 1019 1028
4. CT Chien PL Bartel R Sternglanz S Fields 1991 The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest Proc Natl Acad Sci USA 88 9578 9582
5. E Chuang D Barnard L Hettich XF Zhang J Avruch MS Marshall 1994 Critical binding and regulatory interactions between Ras and Raf occur through a small, stable N-terminal domain of Raf and specific Ras effector residues Mol Cell Biol 14 5318 5325
6. Z Ding J Liang Y Lu Q Yu Z Songyang SY Lin GB Mills 2006 A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners Proc Natl Acad Sci USA 103 15014 15019
7. SK Dove RK McEwen A Mayes DC Hughes JD Beggs RH Michell 2002 Vac14 controls PtdIns(3,5)P(2) synthesis and Fab1-dependent protein trafficking to the multivesicular body Curr Biol 12 885 893
8. RM Eglen R Singh 2003 Beta galactosidase enzyme fragment complementation as a novel technology for high throughput screening Comb Chem High Throughput Screen 6 381 387
9. S Fields O Song 1989 A novel genetic system to detect protein-protein interactions Nature 340 245 246
10. M Fridman H Maruta J Gonez F Walker H Treutlein J Zeng A Burgess 2000 Point mutants of c-raf-1 RBD with elevated binding to v-Ha-Ras J Biol Chem 275 30363 30371
11. JC Game JG Little RH Haynes 1975 Yeast mutants sensitive to trimethoprim Mutat Res 28 175 182
12. K Goishi K Mizuno H Nakanishi T Sasaki 2004 Involvement of Rab27 in antigen-induced histamine release from rat basophilic leukemia 2H3 cells Biochem Biophys Res Commun 324 294 301
13. D Hanahan 1983 Studies on transformation of Escherichia coli with plasmids J Mol Biol 166 557 580
14. C Herrmann GA Martin A Wittinghofer 1995 Quantitative analysis of the complex between p21ras and the Ras-binding domain of the human Raf-1 protein kinase J Biol Chem 270 2901 2905
15. T Huang BJ Barclay TI Kalman RC von Borstel PJ Hastings 1992 The phenotype of a dihydrofolate reductase mutant of Saccharomyces cerevisiae Gene 121 167 71
16. H Ito Y Fukuda K Murata A Kimura 1983 Transformation of intact yeast cells treated with alkali cations J Bacteriol 153 163 168
17. K Kuroda M Kato J Mima M Ueda 2006 Systems for the detection and analysis of protein-protein interactions Appl Microbiol Biotechnol 71 127 136
18. ML MacDonald J Lamerdin S Owens BH Keon GK Bilter Z Shang Z Huang H Yu J Dias T Minami SW Michnick JK Westwick 2006 Identifying off-target effects and hidden phenotypes of drugs in human cells Nat Chem Biol 2 329 337
19. SW Michnick 2003 Protein fragment complementation strategies for biochemical network mapping Curr Opin Biotechnol 14 610 617
20. A Miyajima I Miyajima K Arai N Arai 1984 Expression of plasmid R388-encoded type II dihydrofolate reductase as a dominant selective marker in Saccharomyces cerevisiae Mol Cell Biol 4 407 414
21. K Mizuno A Kitamura T Sasaki 2003 Rabring7, a novel Rab7 target protein with a RING finger motif Mol Biol Cell 14 3741 3752
22. MJ Neves S Hohmann W Bell F Dumortier K Luyten J Ramos P Cobbaert W de Koning Z Kaneva JM Thevelein 1995 Control of glucose influx into glycolysis and pleiotropic effects studied in different isogenic sets of Saccharomyces cerevisiae mutants in trehalose biosynthesis Curr Genet 27 110 122
23. O Nord A Gustrin PA Nygren 2005 Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation FEMS Microbiol Lett 242 73 79
24. N Okochi M Kato-Murai T Kadonosono M Ueda 2007 Design of a serine protease-like catalytic triad on an antibody light chain displayed on the yeast cell surface Appl Microbiol Biotechnol 77 597 603
25. JN Pelletier FX Campbell-Valois SW Michnick 1998 Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments Proc Natl Acad Sci USA 95 12141 12146
26. I Remy SW Michnick 1999 Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays Proc Natl Acad Sci USA 96 5394 5399
27. JE Scheffler DS Waugh E Bekesi SE Kiefer JE LoSardo A Neri KM Prinzo KL Tsao B Wegrzynski SD Emerson DC Fry 1994 Characterization of a 78-residue fragment of c-Raf-1 that comprises a minimal binding domain for the interaction with Ras-GTP J Biol Chem 269 22340 22346
28. S Shibasaki K Kuroda H Duc Nguyen T Mori W Zou M Ueda 2006 Detection of protein-protein interactions by a combination of a novel cytoplasmic membrane targeting system of recombinant proteins and fluorescence resonance energy transfer Appl Microbiol Biotechnol 70 451 457
29. R Subramaniam D Desveaux C Spickler SW Michnick N Brisson 2001 Direct visualization of protein interactions in plant cells Nat Biotechnol 19 769 772
30. M Tajima Y Nogi T Fukasawa 1985 Primary structure of the Saccharomyces cerevisiae GAL7 gene Yeast 1 67 77
31. K Tarassov V Messier CR Landry S Radinovic MM Molina I Shames Y Malitskaya J Vogel H Bussey SW Michnick 2008 An in vivo map of the yeast protein interactome Science 320 1465 1470
32. AS Zervos J Gyuris R Brent 1993 Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites Cell 72 223 232