Design of a serine protease-like catalytic triad on an antibody light chain displayed on the yeast cell surface

Applied Microbiology and Biotechnology

Volumn 77, Issue 3, 2007, Pages 597-603

  Okochi, Norihiko ^a   Kato Murai, Michiko ^a   Kadonosono, Tetsuya ^a   Ueda, Mitsuyoshi ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Catalytic triad; Cell surface display; Proteolytic antibody light chain; Site directed mutagenesis

Indexed keywords

ANTIBODIES; CATALYST ACTIVITY; ENZYME INHIBITION; HYDROLYSIS; MUTAGENESIS; YEAST;

CATALYTIC TRIAD; CELL SURFACE DISPLAY; PROTEOLYTIC ANTIBODY LIGHT CHAIN; SITE DIRECTED MUTAGENESIS;

ENZYME ACTIVITY;

AMINO ACID; ANTIBODY; ASPARTIC ACID; CASEIN; DYFLOS; HISTIDINE; PEPTIDE; SERINE; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; VASOACTIVE INTESTINAL POLYPEPTIDE; VASOACTIVE INTESTINAL POLYPEPTIDE ANTIBODY;

ANTIBODY; CATALYSIS; ENZYME ACTIVITY; HYDROLYSIS; MUTATION; PEPTIDE; YEAST;

ARTICLE; CATALYSIS; CATALYST; CELL SURFACE; CELL SUSPENSION; CONTROLLED STUDY; FUNGAL STRAIN; FUNGUS MUTANT; LIGHT CHAIN; NONHUMAN; PROTEIN DEGRADATION; PROTEIN HYDROLYSIS; PROTEIN STRUCTURE; WILD TYPE; YEAST CELL;

AMINO ACID SEQUENCE; ANTIBODIES, CATALYTIC; CATALYSIS; ESCHERICHIA COLI; IMMUNOGLOBULIN LIGHT CHAINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES; VASOACTIVE INTESTINAL PEPTIDE;

EID: 36248962147     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1197-0     Document Type: Article

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