Inhibition of Cryptosporidium parvum infection of a mammalian cell culture by recombinant scFv antibodies

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Volumn 94, Issue 3, 2008, Pages 353-364

  Pokorny, Nicholas J ^a   Boulter Bitzer, Jeanine I ^a   Hall, Christopher J ^a   Trevors, Jack T ^a   Lee, Hung ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Cryptosporidium; Focidetection method; Immunotherapy; Phagedisplay; Recombinantantibody; scFv

Indexed keywords

BETA GALACTOSIDASE; DNA; GLYCINE; RECOMBINANT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; WATER;

ANTIBODY LIBRARY; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; BACTERIAL STRAIN; CELL CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; DNA SEQUENCE; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENETIC VARIABILITY; HUMAN; HUMAN CELL; IMMUNOTHERAPY; INCUBATION TEMPERATURE; INCUBATION TIME; INHIBITION KINETICS; MAMMAL CELL; MOLECULAR CLONING; MONOLAYER CULTURE; OOCYST; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STOP CODON; TISSUE CULTURE;

ANIMALS; ANTIBODIES, PROTOZOAN; BASE SEQUENCE; CELL LINE; CODON, TERMINATOR; CRYPTOSPORIDIUM PARVUM; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; MUTAGENESIS, SITE-DIRECTED; NEUTRALIZATION TESTS; OOCYSTS; PEPTIDE LIBRARY; RECOMBINANT PROTEINS;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; MAMMALIA;

EID: 50849087339     PISSN: 00036072     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10482-008-9252-0     Document Type: Article

References (30)

1. Baecher-Allan CM, Santora K, Sarantopoulos S, Den W, Sompuram SR, Sharon J, Cevallos AM, Bhat N, Ward H (1999) Generation of a polyclonal Fab phage display library to the protozoan parasite Cryptosporidium parvum. Comb Chem High Throughput Screen 2:319-325
2. Barbas CF, Burton DR, Scott JK, Silverman GJ (2001) Phage display: A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
3. Barik S (1996) Site-directed mutagenesis in vitro by megaprimer PCR. Methods Mol Biol 57:203-215
4. Carey CM, Lee H, Trevors JT (2004) Biology, persistence and detection of Cryptosporidium parvum and Cryptosporidium hominis oocyst. Water Res 38:818-862
5. Carreno RA, Pokorny NJ, Lee H, Trevors JT, DeGrandis SA (2001a) Phenotypic and genotypic characterization of Cryptosporidium species and isolates. J Ind Microbiol Biotechnol 26:95-106
6. Carreno RA, Pokorny NJ, Weir SC, Lee H, Trevors JT (2001b) Decrease in Cryptosporidium parvum oocyst infectivity in vitro by using the membrane filter dissolution method for recovering oocysts from water samples. Appl Environ Microbiol 67:3309-3313
7. Chen L, Williams BR, Yang CY, Cevallos AM, Bhat N, Ward H, Sharon J (2003) Polyclonal Fab phage display libraries with a high percentage of diverse clones to Cryptosporidium parvum glycoproteins. Int J Parasitol 33:281-291
8. Cevallos AM, Bhat N, Verdon R, Hamer DH, Stein B, Tzipori S, Pereira ME, Keusch GT, Ward HD (2000) Mediation of Cryptosporidium parvum infection in vitro by mucin-like glycoproteins defined by a neutralizing monoclonal antibody. Infect Immun 68:5167-5175
9. de Wildt RM, van Venrooij WJ, Winter G, Hoet RM, Tomlinson IM (1999) Somatic insertions and deletions shape the human antibody repertoire. J Mol Biol 294:701-710
10. de Wildt RM, Mundy CR, Gorick BD, Tomlinson IM (2000) Antibody arrays for high-throughput screening of antibody-antigen interactions. Nat Biotechnol 18:989-994
11. Doyle PS, Crabb J, Petersen C (1993) Anti- Cryptosporidium parvum antibodies inhibit infectivity in vitro and in vivo. Infect Immun 61:4079-4084
12. Elliot BC, Wisnewski AV, Johnson J, Fenwick-Smith D, Wiest P, Hamer D, Kresina T, Flanigan TP (1997) In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies. Infect Immun 65:3933-3935
13. Fayer R, Trout JM, Jenkins MC (1998) Infectivity of Cryptosporidium parvum oocysts stored in water at environmental temperatures. J Parasitol 84:165-169
14. Goletz S, Christensen PA, Kristensen P, Blohm D, Tomlinson I, Winter G, Karsten U (2002) Selection of large diversities of anti-idiotypic antibody fragments by phage display. J Mol Biol 315:1087-1097
15. Griffiths AD, Williams SC, Hartley O, 15 other authors (1994) Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J 13:3245-3260
16. Kobayashi C, Yokoyama H, Nguyen SV, Kodama Y, Kimata T, Izeki M (2004) Effect of egg yolk antibody on experimental Cryptosporidium parvum infection in scid mice. Vaccine 23:232-235
17. Kristensen P, Winter G (1998) Proteolytic selection for protein folding using filamentous bacteriophages. Fold Des 3:321-328
18. Korich DG, Mead JR, Madore MS, Sinclair NA, Sterling CR (1990) Effects of ozone, chlorine dioxide, chlorine and monochloramine on Cryptosporidium parvum oocyst viability. Appl Environ Microbiol 56:1423-1428
19. Marks JD, Hoogenboom HR, Bonnert TP, McCafferty J, Griffths AD, Winter G (1991) By-passing immunisation. Human antibodies from V-gene libraries displayed on phage. J Mol Biol 222:581-597
20. Pokorny NJ, Weir SC, Carreno RA, Trevors JT, Lee H (2002) Influence of temperature on Cryptosporidium parvum oocyst infectivity in river water samples as detected by tissue culture assay. J Parasitol 88:641-643
21. Pokorny NJ, Boulter-Bitzer JI, Hall JC, Trevors JT, Lee H (2007) Recombinant antibodies for pathogen detection and immunotherapy. In: Gad SC (ed) Handbook of pharmaceutical biotechnology. John Wiley & Sons, Inc., Hoboken, NJ
22. Rochelle PA, Marshall MM, Mead JR, Johnson AM, Korich DG, Rosen JS, De Leon R (2002) Comparison of in vitro cell culture and a mouse assay for measuring infectivity of Cryptosporidium parvum. Appl Environ Microbiol 68:3809-3817
23. Sambrook J, Russell DW (2001). Molecular cloning: A laboratory manual, 3rd edn.
24. Slifko TR, Friedman D, Rose JB, Jakubowski W (1997) An in vitro method for detecting infectious Cryptosporidium oocysts with cell culture. Appl Environ Microbiol 63:3669-3675
25. Slifko TR, Huffman DE, Dussert BW, Owens JH, Jakubowski W, Haas CN, Rose JB (2002) Comparison of tissue culture and animal models for assessment of Cryptosporidium parvum infection. Exp Parasitol 101:97-106
26. Stibbs HH, Ongerth JE (1986) Immunofluorescence detection of Cryptosporidium oocysts in fecal smears. J Clin Micrbiol 24:517-521
27. Stewart CS, MacKenzie CR, Hall JC (2007) Isolation, characterization and pentamerization of a-cobrotoxin specific single-domain antibodies from a naive phage displaylibrary: Preliminary findings for antivenom development. Toxicon 49:699-709
28. Weir SC, Pokorny NJ, Carreno RA, Trevors JT, Lee H (2001) Improving the rate of infectivity of Cryptosporidium parvum oocysts in cell culture using centrifugation. J Parasitol 87:1502-1504
29. Weir C, Vesey G, Slade M, Ferrari B, Veal DA, Williams K (2000) An immunoglobulin G1 monoclonal antibody highly specific to the wall of Cryptosporidium oocysts. Clin Diagn Lab Immunol 7:745-750
30. Yau KYF, Lee H, Hall JC (2003) Emerging trends in the synthesis and improvement of hapten-specific recombinant antibodies. Biotech Adv 21:599-637