Real-time polymerase chain reaction monitoring of recombinant DNA entry into soil from decomposing roundup ready leaf biomass

Journal of Agricultural and Food Chemistry

Volumn 56, Issue 15, 2008, Pages 6339-6347

  Levy Booth, David J ^a   Campbell, Rachel G ^a   Gulden, Robert H ^a   Hart, Miranda M ^a   Powell, Jeff R ^a   Klironomos, John N ^a   Pauls, K Peter ^a   Swanton, Clarence J ^a   Trevors, Jack T ^a   Dunfield, Kari E ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Agriculture; Biology; Cp4 epsps; Degradation; DNA; Environment; Glyphosate tolerant; Le1; Methodology; Molecular beacon; Real time PCR; Recombinant; Roundup Ready; Soil; Soybean; Transgenic plant

Indexed keywords

DRUG DERIVATIVE; GLYCINE; GLYPHOSATE; PLANT DNA; PLANT LECTIN; RECOMBINANT DNA; SOYBEAN LECTIN; SOYBEAN PROTEIN;

ARTICLE; DRUG TOLERANCE; GENETICS; PLANT LEAF; POLYMERASE CHAIN REACTION; SOIL; SOYBEAN; TRANSGENIC PLANT;

DNA, PLANT; DNA, RECOMBINANT; DRUG TOLERANCE; GLYCINE; PLANT LEAVES; PLANT LECTINS; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; SOIL; SOYBEAN PROTEINS; SOYBEANS;

GLYCINE MAX;

EID: 50449108088     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf800767g     Document Type: Article

References (41)

1. Brookes, G.; Barfoot, P. GM Crops: The Fust Ten Years - Global Socio-Economic and Environmental Impacts; ISAAA Brief No. 36; ISAAA: Ithaca, NY, 2006.
2. James, C. Preview: Global Status of Commercialized Biotech/GM Crops: 2006; ISAAA Brief 35; ISAAA: Ithaca, NY, 2006.
3. Duke, S. O. Taking stock of herbicide-resistant crops ten years after introduction. Pest Manage. Sci. 2005, 61, 211-218.
4. Padgette, S. R.; Kolacz, K. H.; Delannay, X.; Re, D. B.; LaVallee, B. J.; Tinius, C. N.; Rhodes, W. K.; Otero, Y. I.; Barry, G. F.; Eichholtz, D. A.; Peschke, V. M.; Nida, D. L.; Taylor, N. B.; Kishore, G. M. Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci. 1995, 35, 1451-1461.
5. Barry, G. F.; Kishore, G. M.; Padgette, S. R.; Taylor, M.; Kolacz, K. H.; Weldon, M.; Re, D. B.; Eichholtz, D. E.; Fincher, K.; Hallas, L. Inhibitors of amino acid biosyuiesis: Strategies for imparting glyphosate tolerance to crop plants. In Biosynthesis and Molecular Regulation of Amino Acids in Plants; Flores, H. E., Singh, B. K., Shannon, J. C., Eds.; American Society of Plant Physiologists: Rockville, MD, 1992; pp 139-145.
6. Lerat, S.; England, L. S.; Vincent, M. L.; Pauls, K. P.; Swanton, C. J.; Klironomos, J. N.; Trevors, J. T. Real-time polymerase chain reaction (PCR) quantification of the transgenes for Roundup Ready com and Roundup Ready soybean in soil samples. J. Agric. Food Chem. 2005, 53, 1337-1342.
7. Gulden, R. H.; Lerat, S.; Hart, M. M.; Powell, J. T.; Trevors, J. T.; Pauls, K. P.; Klironomos, J. N.; Swanton, C. J. Quantitation of transgenic plant DNA in leachate waten Real-time polymerase chain reaction analysis. J. Agric. Food Chem. 2005, 53, 5858-5865.
8. Levy-Boom, D. J.; Campbell, R. G.; Gulden, R. H.; Hart, M. M.; Powell, J. R.; Klironomos, J. N.; Pauls, K. P.; Swanton, C. J.; Trevors, J. T.; Dunfield, K. E. Cycling of extracellular DNA in the soil environment. Soil Biol. Biochem. 2007, 39, 2977-2991.
9. Snow, A. A.; Andow, D. A.; Gepts, P.; Hallerman, E. M.; Power, A.; Tiedje, J. M.; Wolfenbarger, L. L. Genetically engineered organisms and the environment: current status and recommendations. Ecol. Appl. 2005, 15, 377-404.
10. Greaves, M. P.; Wilson, M. J. The adsorption of nucleic acids by montmoruTonite. Soil Biol. Biochem. 1969, 1, 317-323.
11. Greaves, M. P.; Wilson, M. J. The degradation of nucleic acids and montmorillonite-nucleic-acid complexes by soil microorganisms. Soil Biol. Biochem. 1970, 2, 257-268.
12. Lorenz, M. G.; Wackernagel, W. Adsorption of DNA to sand and variable degradation rates of adsorbed DNA. Appl. Environ. Microbiol. 1987, 53, 2948-2952.
13. Romanowski, G.; Lorenz, M. G.; Wackernagel, W. Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. Appl. Environ. Microbiol. 1991, 57, 1057-1061.
14. Crecchio, C.; Stotzky, G. Binding of DNA on humic acids: Effect on transformation of Bacillus subtilis and resistance to DNase. Soil Biol. Biochem. 1998, 30, 1060-1067.
15. Crecchio, C.; Ruggiero, P.; Curci, M.; Colombo, C.; Palumbo, G.; Stotzky, G. Binding of DNA from Bacillus subtilis on montmorillonite-humic acids-aluminum or iron hydroxypolymers. Soil Sci. Soc. Am. J. 2005, 69, 834-841.
16. Blum, S. A. E.; Lorenz, M. G.; Wackernagel, W. Mechanism of retarded DNA degradation and prokaryotic origin of DNases in nonsterile soils. Syst. Appl. Microbiol. 1997, 20, 513-521.
17. Widmer, F.; Seidler, R. J.; Donegan, K. K.; Reed, G. L. Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol. 1997, 6, 1-7.
18. Gebhard, F.; Smalla, K. Monitoring field releases of transgenic modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbiol. Ecol. 1999, 28, 261-272.
19. Lopez, P.; Espinosa, M.; Strassi, D. L.; Lacks, S. A. Facilitation of plasmid transfer in Streptococcus pneumoniae by chromosomal homology. J. Bacteriol. 1982, 150, 692-701.
20. Carlson, C.; Pierson, L. S.; Rosen, J. J.; Ingraham, J. L. Pseudomonas stutzeri and related species undergo natural transformation. J. Bacteriol. 1983, 153, 93-99.
21. Carlson, C. A.; Steenbergen, S. A.; Ingraham, J. L. Natural transformation of Pseudomonas stutzeri by plasmids that contain cloned fragments of chromosomal deoxyribonucleic acid. Arch. Microbiol. 1984, 40, 134-138.
22. de Vries, J.; Wackernagel, W. Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 2094-2099.
23. Ceccherini, M.; Poté, J.; Kay, E.; Van, V. T.; Marechal, J.; Pietramellara, G.; Nannipieri, P.; Vogel, T. M.; Simonet, P. Degradation and transformability of DNA from transgenic leaves. Appl. Environ. Microbiol. 2003, 69, 673-678.
24. Poté, J.; Rossé, P.; Rosselli, W.; Van, V. T.; Wildi, W. Kinetics of mass and DNA decomposition in tomato leaves. Chemosphere 2005, 61, 677-684.
25. Prakash, C. S. The genetically modified crop debate in the context of agricultural evolution. Plant Physiol. 2001, 126, 8-15.
26. Goldstein, I. J.; Hayes, C. E. The lectins: Carbohydrate-binding proteins of plants and animals. Adv. Carbohydr. Chem. Biochem. 1978, 35, 127-340.
27. Flores, S.; Saxena, D.; Stotzky, G. Transgenic Bt plants decompose less in soil than non-Bt plants. Soil Biol. Biochem. 2005, 37, 1073-1082.
28. Gulden, R. H.; Levy-Booth, D.; Campbell, R.; Powell, J. R.; Hart, M. M.; Dunfield, K. E.; Trevors, J. T.; Pauls, K. P.; Klironomos, J. N.; Swanton, C. J. An empirical approach to target DNA quantification in environmental samples using real-time polymerase chain reactions. Soil Biol. Biochem. 2007, 39, 1956-1967.
29. Jenny, H.; Gessel, S. P.; Bingham, F. T. Comparative study of decomposition rates of organic matter in temperate and tropical regions. Soil Sci. 1949, 68, 419-432.
30. Olson, J. S. Energy storage and the balance of producers and decomposers in ecological systems. Ecology 1963, 44, 322-331.
31. Howard, P. J. A.; Howard, D. M. Microbial decomposition of tree and shrub leaf litter. 1. Weight loss and chemical composition of decomposing litter. Oikos 1974, 25, 341-352.
32. Wieder, R. K.; Lang, G. E. A critique of the analytical methods used in examining decomposition data obtained from litterbags. Ecology 1982, 63, 1636-1642.
33. Pérez-Corona, M. E.; Hernandez, M. C. P.; de Castro, F. B. Decomposition of alder, ash, and poplar litter in a Mediterranean riverine area. Commun. Soil Sci. Plant Anal. 2006, 37, 1111-1126.
34. Goldberg, R. B.; Hoschek, G.; Vodkin, L. O. An insertion sequence blocks the expression of a soybean lectin gene. Cell 1983, 33, 465-475.
35. Dilly, O.; Bloem, J.; Vos, A.; Munch, J. C. Bacterial diversity in agricultural soils during litter decomposition. Appl. Environ. Microbiol. 2004, 70, 468-474.
36. Kumar, K.; Goh, K. M. Crop residues and management practices: Effects on soil quality, soil nitrogen dynamics, crop yield, and nitrogen recovery. In Advances in Agronomy; Sparks, D. L., Ed.; Academic Press: London, 2000; Vol. 66, pp 197-319.
37. Khanna, M.; Stotzky, G. Transformation of Bacillus subtilis by DNA bound on montmorillonite and effect of DNase on the transforming ability of bound DNA. Appl. Environ. Microbiol. 1992, 58, 1930-1939.
38. Pietramellara, G.; Franchi, M.; Gallon, E.; Nannipieri, P. Effect of molecular characteristics of DNA on its adsorption and binding on homoionic montmorillonite and kaolinite. Biol. Fertil. Soils 2001, 33, 402-409.
39. Romanowski, G.; Lorenz, M. G.; Sayler, G.; Wackernagel, W. Persistence of free plasmid DNA in soil monitored by various methods, including a transformation assay. Appl. Environ. Microbiol. 1992, 58, 3012-3019.
40. Herdina; Neate, S.; Jabaji-Hare, S.; Ophel-Keller, K. Persistence of DNA of Gaeumannomyces graminis var. tritici in soil as measured by a DNA-based assay. FEMS Microbiol. Ecol. 2004, 47, 143-152.
41. Lo, C.; Chen, S.; Yang, J. Use of real-time polymerase reaction (PCR) and transformation assay to monitor the persistence and bioavailability of transgenic genes released form genetically modified papaya expressing nptII and PRSV genes in the soil. J. Agric. Food Chem. 2007, 55, 7534-7540.