Biomarker (ProEx™ C, p16INK4A, and MiB-1) distinction of high-grade squamous intraepithelial lesion from its mimics

Modern Pathology

Volumn 21, Issue 9, 2008, Pages 1067-1074

  Pinto, Alvaro P ^a   Schlecht, Nicolas F ^b   Y C Woo, Terri ^c   Crum, Christopher P ^c   Cibas, Edmund S ^c  

^a FEDERAL UNIVERSITY OF PARANÁ   (Brazil)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

16INK4A; Cervical biopsy; Cervical intraepithelial neoplasia; High grade squamous intraepithelial lesion; MiB 1; ProEx C

Indexed keywords

BIOLOGICAL MARKER; CELL CYCLE PROTEIN; CYCLIN DEPENDENT KINASE INHIBITOR 2A; DNA TOPOISOMERASE (ATP HYDROLYSING); DNA TOPOISOMERASE II ALPHA; KI 67 ANTIGEN; MINICHROMOSOME MAINTENANCE PROTEIN 2; PROEX C; DNA BINDING PROTEIN; MCM2 PROTEIN, HUMAN; MIB1 PROTEIN, HUMAN; NUCLEAR PROTEIN; TUMOR ANTIGEN; TUMOR MARKER; UBIQUITIN PROTEIN LIGASE;

ARTICLE; CELL CYCLE S PHASE; COMPARATIVE STUDY; CONTROLLED STUDY; CORRELATION ANALYSIS; DIFFERENTIAL DIAGNOSIS; HIGH GRADE SQUAMOUS INTRAEPITHELIAL LESION; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; UTERINE CERVIX BIOPSY; UTERINE CERVIX CARCINOMA IN SITU; ATROPHY; CELL NUCLEUS; CHEMISTRY; CYTOPLASM; EVALUATION; FEMALE; METAPLASIA; PATHOLOGY; ROC CURVE; UTERINE CERVIX; UTERINE CERVIX TUMOR;

ANTIGENS, NEOPLASM; ATROPHY; CELL CYCLE PROTEINS; CELL NUCLEUS; CERVICAL INTRAEPITHELIAL NEOPLASIA; CERVIX UTERI; CYCLIN-DEPENDENT KINASE INHIBITOR P16; CYTOPLASM; DIAGNOSIS, DIFFERENTIAL; DNA TOPOISOMERASES, TYPE II, EUKARYOTIC; DNA-BINDING PROTEINS; FEMALE; HUMANS; METAPLASIA; NUCLEAR PROTEINS; ROC CURVE; TUMOR MARKERS, BIOLOGICAL; UBIQUITIN-PROTEIN LIGASES; UTERINE CERVICAL NEOPLASMS;

EID: 50249171666     PISSN: 08933952     EISSN: 15300285     Source Type: Journal    
DOI: 10.1038/modpathol.2008.101     Document Type: Article

References (21)

1. Park TW, Richart RM, Sun XW, et al. Association between human papillomavirus type and clonal status of cervical squamous intraepithelial lesions. J Natl Cancer Inst 1996;88:355-358.
2. Crum CP, Mitao M, Levine RU, et al. Cervical papillomaviruses segregate within morphologically distinct precancerous lesions. J Virol 1985;54:675-681.
3. Genest DR, Stein L, Cibas E, et al. A binary (Bethesda) system for classifying cervical cancer precursors: criteria, reproducibility, and viral correlates. Hum Pathol 1993;24:730-736.
4. Stoler MH, Schiffman M, Atypical Squamous Cells of Undetermined Significance-Low-grade Squamous Intraepithelial Lesion Triage Study (ALTS) Group. Interobserver reproducibility of cervical cytologic and histologic interpretations: realistic estimates from the ASCUS-LSILTriage Study. JAMA 2001;285:1500-1505.
5. Crum CP, Rose PG. Cervical squamous neoplasia. In: Crum CP, Lee KR (eds). Diagnostic Gynecologic and Obstetric Pathology, 1st edn. Elsevier Saunders: Philadelphia, PA, 2006, pp 306-310.
6. Keating JT, Cviko A, Riethdorf S, et al. Ki-67, cyclin E, and p16INK4 are complimentary surrogate biomarkers for human papilloma virus-related cervical neoplasia. Am J Surg Pathol 2001;25:884-891.
7. Chen Y, Miller C, Mosher R, et al. Identification of cervical cancer markers by cDNA and tissue microarrays. Cancer Res 2003;63:1927-1935.
8. Shi SR, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 1991;39:741-748.
9. Klaes R, Benner A, Friedrich T, et al. p16INK4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. Am J Surg Pathol 2002;26:1389-1399.
10. Shi J, Liu H, Wilkerson M, et al. Evaluation of p16INK4a, minichromosome maintenance protein 2, DNA topoisomerase IIalpha, ProE(X) C, and p16INK4a/ProE(X) C in cervical squamous intraepithelial lesions. Hum Pathol 2007;38:1335-1344.
11. DeLong ER, DeLong DM, Clarke-Pearson DL. Comparing the areas under two or more correlated receiver operating curves: a nonparametric approach. Biometrics 1988;44:837-845.
12. Lorenzato M, Caudroy S, Bronner C, et al. Cell cycle and/or proliferation markers: what is the best method to discriminate cervical high-grade lesions? Hum Pathol 2005;36:1101-1107.
13. Akpolat I, Smith DA, Ramzy I, et al. The utility of p16INK4a and Ki-67 staining on cell blocks prepared from residual thin-layer cervicovaginal material. Cancer 2004;102:142-149.
14. Sahebali S, Depuydt CE, Boulet GA, et al. Immunocytochemistry in liquid-based cervical cytology: analysis of clinical use following a cross-sectional study. Int J Cancer 2006;118:1254-1260.
15. Moore GD, Lear SC, Wills-Frank LA, et al. Differential expression of cdk inhibitors p16, p21cip1, p27kip1, and cyclin E in cervical cytological smears prepared by the ThinPrep method. Diagn Cytopathol 2005;32:82-87.
16. Kelly D, Kincaid E, Fansler Z, et al. Detection of cervical high-grade squamous intraepithelial lesions from cytologic samples using a novel immunocyto-chemical assay (ProEx C). Cancer 2006;108:494-500.
17. Shroyer KR, Homer P, Heinz D, et al. Validation of a novel immunocytochemical assay for topoisomerase II-alpha and minichromosome maintenance protein 2 expression in cervical cytology. Cancer 2006;108:324-330.
18. Goel MM, Mehrotra A, Singh U, et al. MIB-1 and PCNA immunostaining as a diagnostic adjunct to cervical Pap smear. Diagn Cytopathol 2005;33:15-19.
19. Kruse AJ, Baak JP, Janssen EA, et al. Ki67 predicts progression in early CIN: validation of a multivariate progression-risk model. Cell Oncol 2004;26:13-20.
20. Kruse AJ, Baak JP, Janssen EA, et al. Low- and high-risk CIN 1 and 2 lesions: prospective predictive value of grade, HPV, and Ki-67 immuno-quantitative variables. J Pathol 2003;199:462-470.
21. Kruse AJ, Skaland I, Janssen EA, et al. Quantitative molecular parameters to identify low-risk and high-risk early CIN lesions: role of markers of proliferative activity and differentiation and Rb availability. Int J Gynecol Pathol 2004;23:100-109.