Optimized expression and purification of myristoylated human neuronal calcium sensor 1 in E. coli

Protein Expression and Purification

Volumn 61, Issue 2, 2008, Pages 103-112

  De Cotiis, Dan A ^a   Woll, Matthew P ^a   Fox, Todd E ^a   Hill, R Blake ^b   Levenson, Robert ^a   Flanagan, John M ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

NCS 1; neuronal calcium sensor 1

Indexed keywords

ACYLTRANSFERASE; CALCIUM; FREQUENIN CALCIUM SENSOR PROTEINS; GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE; ISOPROTEIN; LIPID; MYRISTIC ACID; NEURONAL CALCIUM SENSOR; NEUROPEPTIDE; PROTEIN N MYRISTOYLTRANSFERASE;

ARTICLE; BINDING SITE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE TRANSFER; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; STANDARD; TIME; YEAST;

ACYLTRANSFERASES; BINDING SITES; CALCIUM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE TRANSFER TECHNIQUES; HUMANS; LIPIDS; MAGNETIC RESONANCE SPECTROSCOPY; MYRISTIC ACID; NEURONAL CALCIUM-SENSOR PROTEINS; NEUROPEPTIDES; PROTEIN ISOFORMS; REFERENCE STANDARDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS; YEASTS;

ESCHERICHIA COLI;

EID: 50049107014     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.06.006     Document Type: Article

References (36)

1. Pongs O., Lindemeier J., Zhu X.R., Theil T., Engelkamp D., Krah-Jentgens I., Lambrecht H.G., Koch K.W., Schwemer J., Rivosecchi R., et al. Frequenin-novel calcium-binding protein that modulates synaptic efficacy in the Drosophila nervous system. Neuron 1 1 (1993) 5-28
2. Nef S., Fiumelli H., de Castro E., Raes M.B., and Nef P. Identification of neuronal calcium sensor (NCS-1) possibly involved in the regulation of receptor phosphorylation. J. Recept. Signal Transduct. Res. 15 1-4 (1995) 65-78
3. Sallese M., Iacovelli L., Cumashi A., Capobianco L., Cuomo L., and De Blasi A. Regulation of G protein-coupled receptor kinase subtypes by calcium sensor proteins. Biochim. Biophys. Acta 1498 2-3 (2000) 112-121
4. Kabbani N., Negyessy L., Lin R., Goldman-Rakic P., and Levenson R. Interaction with neuronal calcium sensor NCS-1 mediates desensitization of the D2 dopamine receptor. J. Neurosci. 22 19 (2002) 8476-8486
5. Weiss J.L., Archer D.A., and Burgoyne R.D. Neuronal Ca2+ sensor-1/frequenin functions in an autocrine pathway regulating Ca2+ channels in bovine adrenal chromaffin cells. J. Biol. Chem. 275 51 (2000) 40082-40087
6. 2+-binding proteins. Biochem. J. 353 (2001) 1-12
7. Ames J.B., Hendricks K.B., Strahl T., Huttner I.G., Hamasaki N., and Thorner J. Structure and calcium-binding properties of Frq1, a novel calcium sensor in the yeast Saccharomyces cerevisiae. Biochemistry 39 40 (2000) 12149-12161
8. Flaherty K.M., Zozulya S., Stryer L., and McKay D.B. Three-dimensional structure of recoverin, a calcium sensor in vision. Cell 75 4 (1993) 709-716
9. Stephen R., Palczewski K., and Sousa M.C. The crystal structure of GCAP3 suggests molecular mechanism of GCAP-linked cone dystrophies. J. Mol. Biol. 359 2 (2006) 266-275
10. Scannevin R.H., Wang K., Jow F., Megules J., Kopsco D.C., Edris W., Carroll K.C., Lü Q., Xu W., Xu Z., Katz A.H., Olland S., Lin L., Taylor M., Stahl M., Malakian K., Somers W., Mosyak L., Bowlby M.R., Chanda P., and Rhodes K.J. Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1. Neuron 41 4 (2004) 587-598
11. Gifford J.L., Walsh M.P., and Vogel H.J. Structures and metal-ion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs. Biochem. J. 405 2 (2007) 199-221
12. Cox J.A., Drussel I., Comte M., Nef S., Nef P., Lenz S.E., and Gundelfinger E.D. Cation binding and conformational changes in VILIP and NCS-1, two neuron-specific calcium-binding proteins. J. Biol. Chem. 269 (1994) 32807-32814
13. Bourne Y., Dannenberg J., Pollmann V., and Marchot P. Pongs Immunochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1). J. Biol. Chem. 276 (2001) 11949-11955
14. O'Callaghan D.W., Ivings L., Weiss J.L., Ashby M.C., Tepikin A.V., and Burgoyne R.D. Differential use of myristoyl groups on neuronal calcium sensor proteins as a determinant of spatio-temporal aspects of Ca2+ signal transduction. J. Biol. Chem. 277 16 (2002) 14227-14237
15. Zhao X., Várnai P., Tuymetova G., Balla A., Tóth Z.E., Oker-Blom C., Roder J., Jeromin A., and Balla T. Interaction of neuronal calcium sensor-1 (NCS-1) with phosphatidylinositol 4-kinase beta stimulates lipid kinase activity and affects membrane trafficking in COS-7 cells. J. Biol. Chem. 276 43 (2001) 40183-40189
16. Muralidhar D., Jobby M.K., Krishnan K., Annapurna V., Chary K.V., Jeromin A., and Sharma Y. Equilibrium unfolding of neuronal calcium sensor-1: N-terminal myristoylation influences unfolding and reduces protein stiffening in the presence of calcium. J. Biol. Chem. 280 16 (2005) 15569-15578
17. Jeromin A., Muralidhar D., Parameswaran M.N., Roder J., Fairwell T., Scarlata S., Dowal L., Mustafi S.M., Chary K.V., and Sharma Y. N-terminal myristoylation regulates calcium-induced conformational changes in neuronal calcium sensor-1. J. Biol. Chem. 279 26 (2004) 27158-27167
18. Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41 1 (2005) 207-234
19. Fisher J.R., Sharma Y., Iuliano S., Piccioti R.A., Krylov D., Hurley J., Roder J., and Jeromin A. Purification of myristoylated and nonmyristoylated neuronal calcium sensor-1 using single-step hydrophobic interaction chromatography. Protein Expr. Purif. 20 1 (2000) 66-72
20. Pace C.N., Vajdos F., Fee L., Grimsley G., and Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4 11 (1995) 2411-2423
21. Ehrlich L.S., Fong S., Scarlata S., Zybarth G., and Carter C. Partitioning of HIV-1 Gag and Gag-related proteins to membranes. Biochemistry 35 13 (1996) 3933-3943
22. Tyler R.C., Sreenath H.K., Singh S., Aceti D.J., Bingman C.A., Markley J.L., and Fox B.G. Auto-induction medium for the production of [U-15N]- and [U-13C, U-15N]-labeled proteins for NMR screening and structure determination. Protein Expr. Purif. 40 2 (2005) 268-278
23. Piotto M., Saudek V., and Sklenár V. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J. Biomol. NMR 2 6 (1992) 661-665
24. Delaglio F., Grzesiek S., Vuister G.W., Zhu G., Pfeifer J., and Bax A. NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR 6 3 (1995) 277-293
25. Johnson B.A. Using NMRView to visualize and analyze the NMR spectra of macromolecules. Methods Mol. Biol. 278 (2004) 313-352
26. E.L. Ulrich, H. Akutsu, J.F. Doreleijers, Y. Harano, Y.E. Ioannidis, J. Lin, M. Livny, S. Mading, D. Maziuk, Z. Miller, E. Nakatani, C.F. Schulte, D.E. Tolmie, R. Kent Wenger, H. Yao, J.L. Markley, BioMagResBank. Nucleic Acids Res. 36 (2008) (Database issue) D402-D408.
27. D.C. Schekar, Available from: . Accessed: 01-15-08.
28. Arnold F.H. Metal-affinity separations: a new dimension in protein processing. Biotechnology (NY) 9 2 (1991) 151-156
29. Porath J. Immobilized metal ion affinity chromatography. Protein Expr. Purif. 3 4 (1992) 263-281
30. Brahms S., and Brahms J. Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism. J. Mol. Biol. 138 2 (1980) 149-178
31. McFerran B.W., Weiss J.L., and Burgoyne R.D. Neuronal Ca(2+) sensor 1. Characterization of the myristoylated protein, its cellular effects in permeabilized adrenal chromaffin cells, Ca(2+)-independent membrane association, and interaction with binding proteins, suggesting a role in rapid Ca(2+) signal transduction. J. Biol. Chem. 274 42 (1999) 30258-30265
32. Aravind P., Chandra K., Reddy P.P., Jeromin A., Chary K.V., and Sharma Y. Regulatory and structural EF-hand motifs of neuronal calcium sensor-1: Mg 2+ modulates Ca 2+ binding, Ca 2+-induced conformational changes, and equilibrium unfolding transitions. J. Mol. Biol. 376 4 (2008) 1100-1115
33. Grynkiewicz G., Poenie M., and Tsien R.Y. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260 6 (1985) 3440-3450
34. Linse S. Calcium binding to proteins studied via competition with chromophoric chelators. Methods Mol. Biol. 173 (2002) 15-24
35. Sharma R.K. Potential role of N-myristoyltransferase in pathogenic conditions. Can. J. Physiol. Pharmacol. 82 10 (2004) 849-859
36. Boutin J.A. Myristoylation. Cell Signal. 9 1 (1997) 15-35