Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB)

Journal of Invertebrate Pathology

Volumn 99, Issue 1, 2008, Pages 8-13

  Han, Sang Hoon ^a   Lee, Do Bu ^a   Lee, Dong Woo ^a   Kim, Eul Hwan ^a   Yoon, Byoung Su ^a  

^a Kyonggi University   (South Korea)

Author keywords

American Foulbrood (AFB); Honeybee; Micro PCR; Paenibacillus larvae; Real time PCR detection; Ultra rapid real time PCR

Indexed keywords

BACTERIAL DNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; BACILLACEAE; BACTERIAL INFECTION; BEE; GENETICS; HOST PATHOGEN INTERACTION; ISOLATION AND PURIFICATION; LARVA; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; TIME;

ANIMALS; BACILLACEAE; BEES; DNA, BACTERIAL; GRAM-POSITIVE BACTERIAL INFECTIONS; HOST-PATHOGEN INTERACTIONS; LARVA; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

APIS MELLIFERA; PAENIBACILLUS; PAENIBACILLUS LARVAE;

EID: 49849084858     PISSN: 00222011     EISSN: 10960805     Source Type: Journal    
DOI: 10.1016/j.jip.2008.04.010     Document Type: Article

References (21)

1. Alippi A.M., Lopez A.C., and Aguilar O.M. A PCR-based method that permits specific detection of Paenibacillus larvae subsp. larvae, the cause of American Foulbrood of honey bees, at the subspecies level. Lett. Appl. Microbiol. 39 (2004) 25-33
2. Bailey L., and Lee D.C. Bacillus larvae: its cultivation in vitro and its growth in vivo. J. Gen. Microbiol. 29 (1962) 711-717
3. Bakonyi T., Derakhshifar I., Grabensteiner E., and Nowotny N. Development and evaluation of PCR assays for the detection of Paenibacillus larvae in honey samples: comparison with isolation and biochemical characterization. Appl. Environ. Microbiol. 69 (2003) 1504-1510
4. Cho Y.K., Kim J.T., Lee Y.S., and Kim Y.A. Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus. Biosens. Bioelectron. 21 (2006) 2161-2169
5. Dobbelaere W., De Graaf D.C., Peeters J.E., and Jacobs F.J. Development of a fast and reliable diagnostic method for American foulbrood disease (Paenibacillus larvae subsp larvae) using a 16S rRNA gene based PCR. Apidologie 32 (2001) 363-370
6. Govan V.A., Allsopp M.H., and Davison S. A PCR detection method for rapid identification of Paenibacillus larvae. Appl. Environ. Microbiol. 65 (1999) 2243-2245
7. Ha J.S., Yoo M.S., Kwon S.H., and Son W.G. Development of multiplex-rapid PCR method for the detection of Melissococcus plutonious, Bacillus subtilis and Paenibacillus alvei. Korean J. Apicul. 21 (2006) 101-106
8. Heid C.A., Stevens J., Kivak K.J., and Williams P.M. Real time quantitative PCR. Genome Res. 6 (1996) 986-994
9. Heyndrickx M., Vandemeulebroecke K., Scheldeman P., and Kersters K. A polyphasic reassessment of the genus Paenibacillus reclassification of Bacillus lautus (Nakamura 1984) as Paenibacillus lautus comb. nov. and of Bacillus peoriae (Montefusco et al. 1993) as Paenibacillus peoriae comb nov., and emended descriptions of P. lautus and of P. peoriae. Int. J. Syst. Bacteriol. 46 (1996) 988-1003
10. Higuchi R., Fockler C., Dollinger G., and Watson R. Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Biotechnology (NY) 11 (1993) 1026-1030
11. Huh B., Ha Y.J., Oh J.T., and Park E.H. A rapid PCR-based assay for detecting hepatitis B viral DNA using GenSpector TMC-1000. Agric. Chem. Biotechnol. 49 (2006) 143-147
12. Kim E.H., Lee D.W., Han S.H., and Kwon S.H. Rapid detection of avian influenza subtype H5N1 using quick real-time PCR. Korean J. Microbiol. 43 (2007) 23-30
13. Lauro F.M., Favaretto M., Covolo L., and Rassu M. Rapid detection of Paenibacillus larvae from honey and hive samples with a novel nested PCR protocol. Int. J. Food Microbiol. 81 (2003) 195-201
14. Lee D.B., Yang O.S., Han S.H., and Lim Y.K. Rapid detection of Paenibacillus larvae larvae caused American Foulbrood using real-time PCR. Korean J. Apicul. 19 (2004) 97-108
15. Lee D.W., Kim E.H., Yoo M.S., and Han S.H. Ultra-rapid real-time PCR for the detection of human immunodeficiency virus (HIV). Korean J. Microbiol. 43 (2007) 91-99
16. Matheson A., and Reid M. Strategies for the prevention and control of American Foulbrood. Am. Bee. J. 133 (1992) 471-475
17. Mello A.J.D. DNA amplification: does small really mean efficient?. Lab Chip 1 (2001) 24N-29N
18. Nazarenko I.A., Bhatnagar S.K., and Hohman R.J. A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res. 25 (1997) 2516-2521
19. Qin X., Evans J.D., Aronstein K.A., and Murray K.D. Genome sequences of the honey bee pathogens Paenibacillus larvae and Ascosphaera apis. Insect Mol. Biol. 15 (2006) 715-718
20. Ratti C., Budge G., Ward L., and Clover G. Detection and realtive quantitation of soil-borne cereal mosaic virus (SBCMV) and Polymyxa graminis in winter wheat using real-time PCR (TaqMan®). J. Virol. Methods 122 (2004) 95-103
21. Yang O.S., and Yoon B.S. Rapid PCR detection of Paenibacillus larvae as the pathogen of American Foulbrood disease by using nucleotide sequence from its metalloprotease and 16S rRNA gene. Korean J. Apicul. 16 (2001) 1-8